Exposure of adult sand dollars (Scaphechinus mirabilis) (Agassiz, 1864) to copper oxide nanoparticles induces gamete DNA damage

Kukla, Sergey Petrovich; Слободскова, Валентина Владимировна; Zhuravel, E. V.; Mazur, Andrey Alexandrovich; Челомин, В. П.

doi:10.1007/s11356-021-18318-0

Cited by 7 publications

(4 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, Slobodskova et al (2019) reported that chronic pollution of the aquatic environment causes destructive changes to DNA in gill and digestive gland cells of C. grayanus from Nakhodka Bay, Sea of Japan (East Sea). Also, the adverse consequences of pollution identified based on the highly sensitive and relevant methods for determining genotoxicity (including those used in our work such as the DNA comet assay), were reported from Hong Kong Bay (Siu et al, 2008), the Vostok Bay (Sea of Japan) (Kukla et al, 2022), waters off the western coast of India (Sarker et al, 2018). DNA damage is of high concern as it can lead to changes at the gene level resulting in mutagenicity or carcinogenicity in fish tissues owing to chronic exposure to heavy metals or PAH etc.…”

Section: Resultsmentioning

confidence: 63%

Monitoring DNA Damage in Suez Pufferfish (Lagocephalus suezensis) from the Northeastern Mediterranean

Turan

Ergenler

Bardakçı³

2022

Natural and Engineering Sciences

View full text Add to dashboard Cite

Industrial effluents, agricultural runoff, and municipal wastewaters contain unknown substances and complex mixtures that are released into the environment and can lead to contamination of surface and subsurface waters. Such activities have endangered the existence of ecosystems and their inhabitants. Changes in the genome caused by genotoxic agents led to mutations and pose a burden to the populations of fish species. In the present study, we have used the alkaline Comet assay to detect the genotoxicity in Suez pufferfish (Lagocephalus suezensis) sampled from two different gulfs (Iskenderun and Mersin Bay), Northeastern Mediterranean. At the end of the study, the damage frequency (%), arbitrary unit and genetic damage index (%) were evaluated in gill and liver cells of L. suezensis by comet assay. The DNA damage in the gill and liver cells of L. suezensis in the present study were observed with a higher level of DNA damage in gill cells compared to liver cells in both the Iskenderun and Mersin Bays. The highest level of DNA damage (55.01±1.02%), arbitrary unit (143.01±7.21) and genetic damage index (1.43±0.07 %) were found in gill cells of L. suezensis from Mersin Bay. Statistically significant differences were found between DNA damage, Arbitrary unit and genetic damage index values of the two locations (p < 0.001). In conclusion, this study indicates that comet assay gives sensitive results in monitoring the pollution, especially the pollution of the gulf, and thus it might be used as a standard method in regularly monitoring the pollution of the coastal ecosystem.

show abstract

Section: Resultsmentioning

confidence: 63%

Monitoring DNA Damage in Suez Pufferfish (Lagocephalus suezensis) from the Northeastern Mediterranean

Turan

Ergenler

Bardakçı³

2022

Natural and Engineering Sciences

View full text Add to dashboard Cite

show abstract

“…The sand dollar S. mirabilis proposed as an object of study is widely distributed in the Far East region, and is well studied and sensitive to the impact of natural and anthropogenic factors [ 33 , 34 , 35 ]. The sand dollar S. mirabilis , as a typical representative of the class of sea urchins, throws out germ cells directly into sea water during the spawning period, where fertilization and further development of embryos and larvae take place.…”

Section: Resultsmentioning

confidence: 99%

Genotoxic Effects of Exposure to Water-Soluble Fraction of Diesel Fuel in Sand Dollar Scaphechinus mirabilis Gametes

Челомин

Слободскова

Kukla

et al. 2022

Toxics

Self Cite

View full text Add to dashboard Cite

Pollution of marine areas with oil and oil products is steadily growing. As part of this connection, the study of the impact of petroleum hydrocarbons on marine hydrobionts is an urgent issue of modern ecotoxicology. In our study, the genotoxic effect of the water-soluble fraction of diesel fuel at different concentrations on the gametes of the sand dollar Scaphechinus mirabilis was evaluated. It was shown that during the incubation of sperm and eggs of a sand dollar in WAF with an oil hydrocarbon content of 1.32; 2.64; 5.37; 7.92 mg/L caused the destruction of the DNA molecule to varying degrees in both types of gametes. In addition, it has been shown that with an increase in the concentration of petroleum hydrocarbons in WAF, a large number of cells with a high level of DNA damage appear. The success of fertilization after exposure of gametes to a water-soluble extract of petroleum hydrocarbons was also evaluated. The relationship between an increase in the concentration of hydrocarbons in the tested solutions and a decrease in the level of fertilization is shown.

show abstract

“…To determine the level of damage in the DNA, we used an alkaline version of the comet assay adapted for hydrobionts [ 39 ].…”

Section: Methodsmentioning

confidence: 99%

Expanded Polystyrene-Debris-Induced Genotoxic Effect in Littoral Organisms

Chelomin,

Dovzhenko,

Slobodskova

et al. 2023

Toxics

Self Cite

View full text Add to dashboard Cite

Expanded polystyrene (EPS) is a major component of plastic debris in the environment, including coastal and littoral zones. EPS is widely used in various industries including fish farming and aquaculture, which poses a serious potential threat not only to cultured hydrobionts but also to all living organisms, including humans. This paper presents the results of experimental studies on the effects of EPS (0.024 m2/L) on marine mollusks Mytilus trossulus and Tegula rustica, which are typical inhabitants of the upper littoral of Peter the Great Bay (Sea of Japan), belonging to different systematic groups and differing in the type of nutrition. The results of biochemical marker analysis showed the development of oxidative stress processes. Thus, increasing malondialdehyde content relative to control values was registered in the digestive glands of M. trossulus and T. rustica. In the cells of the digestive glands of M. trossulus, integral antioxidant activity decreased more than 1.5 times compared with that of the control. The change in the concentration of protein carbonyls was unchanged in M. trossulus, whereas in T. rustica, there was a 1.5-fold increase. EPS exposure also resulted in significant DNA damage in the studied mollusks—the damage level increased 2.5-fold in M. trossulus and 1.5-fold in T. rustica relative to the control, indicating the genotoxic potential of EPS litters.

show abstract

Exposure of adult sand dollars (Scaphechinus mirabilis) (Agassiz, 1864) to copper oxide nanoparticles induces gamete DNA damage

Cited by 7 publications

References 53 publications

Monitoring DNA Damage in Suez Pufferfish (Lagocephalus suezensis) from the Northeastern Mediterranean

Monitoring DNA Damage in Suez Pufferfish (Lagocephalus suezensis) from the Northeastern Mediterranean

Genotoxic Effects of Exposure to Water-Soluble Fraction of Diesel Fuel in Sand Dollar Scaphechinus mirabilis Gametes

Expanded Polystyrene-Debris-Induced Genotoxic Effect in Littoral Organisms

Contact Info

Product

Resources

About