Exposure of adult zebrafish to androstenedione alters thyroid hormone levels and the transcriptional expression of genes related to the hypothalamus-pituitary-thyroid axis

Liang, Yan‐Qiu; Situ, Yanjie; Xie, Linchun; Huo, Jialiang; Dong, Zhongdian; Li, Chengyong; Zhong, Lin

doi:10.1016/j.aqrep.2021.100966

Cited by 2 publications

(3 citation statements)

References 39 publications

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“…In zebrafish larvae, BPAF exposure decreases T3 and T4 levels and affects the expression of hypothalamus‐pituitary axis‐related genes, thereby causing thyroid endocrine disorders 41 . In this study, the expression levels of TH receptors ( trhr2 , trhra , trhrb , and thra ) and a gene related to the differentiation of thyroid follicular cells ( nkx2.1 ) have been evaluated 37,42–46 . Exposure to high concentrations of BPAF (5 mg/L) significantly damaged the thyroid function of embryos.…”

Section: Discussionmentioning

confidence: 99%

“…41 In this study, the expression levels of TH receptors (trhr2, trhra, trhrb, and thra) and a gene related to the differentiation of thyroid follicular cells (nkx2.1) have been evaluated. 37,[42][43][44][45][46] Exposure to high concentrations of BPAF (5 mg/L) significantly damaged the thyroid function of embryos.…”

Section: Bpaf Exposure Affects Thyroid Function In Marine Medaka Embryosmentioning

confidence: 99%

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Toxic effects of bisphenol AF on the embryonic development of marine medaka (Oryzias melastigma)

Huang

Gao

Chen

et al. 2023

Environmental Toxicology

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Bisphenol AF (BPAF), an emerging environmental endocrine disruptor, has been detected in surface waters worldwide and has adverse effects on aquatic organisms. The accumulation of BPAF in oceans and its potential toxic effect on marine organisms are important concerns. In this study, the effects of BPAF (10, 100, 1, and 5 mg/L) on marine medaka (Oryzias melastigma) were evaluated, including effects on the survival rate, heart rate, hatchability, morphology, and gene expression in embryos. The survival rate of marine medaka embryos was significantly lower after treatment with 5 mg/L BPAF than in the solvent control group. Exposure to 1 mg/L and 5 mg/L BPAF significantly reduced hatchability. Low‐dose BPAF (10 μg/L) significantly accelerated the heart rate of embryos, while high‐dose BPAF (5 mg/L) significantly decreased the heart rate. BPAF exposure also resulted in notochord curvature, pericardial edema, yolk sac cysts, cardiovascular bleeding, and caudal curvature in marine medaka. At the molecular level, BPAF exposure affected the transcript levels of genes involved in the thyroid system (dio1, dio3a, trhr2, tg, and thra), cardiovascular system (gata4, atp2a1, and cacna1da), nervous system (elavl3 and gap43), and antioxidant and inflammatory systems (sod, pparβ, and il‐8) in embryos. These results indicate that BPAF exposure can alter the expression of functional genes, induce abnormal development, and reduce the hatching and survival rates in marine medaka embryos. Overall, BPAF can adversely affect the survival and development of marine medaka embryos, and BPAF may not be an ideal substitute for BPA.

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Section: Discussionmentioning

confidence: 99%

Section: Bpaf Exposure Affects Thyroid Function In Marine Medaka Embryosmentioning

confidence: 99%

Toxic effects of bisphenol AF on the embryonic development of marine medaka (Oryzias melastigma)

Huang

Gao

Chen

et al. 2023

Environmental Toxicology

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“…Androstenedione levels of 2.3 and 23.7 μg/L have been shown to disrupt thyroid function in zebrafish, which in turn affects growth, metabolism, and reproduction (Liang et al, 2021). Male mosquitoes can be hypermasculinized and female mosquitoes masculinized by long-term exposure to water containing 0.4 and 4 μg/L of androstenedione, respectively (Hou et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Trace Analysis of Five Androgens in Environmental Waters by Optimization of Enzymolysis and Solid‐Phase Extraction Ultra‐Performance Liquid Chromatography–Mass Spectrometry and its Risk Assessment

Xie,

Gao,

Ren

2024

Enviro Toxic and Chemistry

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Steroid hormones (SHs), have received widespread attention in recent years. However, current studies of SHs have primarily focused on estrogenic substances and androgen‐related studies are still very limited. In this study, the solid‐phase extraction (SPE) pre‐treatment method, as well as the enzymolysis conditions of 5 androgenic materials, such as Androstenedione, Boldenone, Methandienone, Nandrolone, and Testosterone, were optimized to simultaneously determine their concentrations in the effluent from wastewater treatment plants (WWTP) and surface water samples. Then, the ecological risks of the 5 androgens in the effluent and Pearl River basin of Guangzhou were evaluated with the risk quotient RQ method. The results showed that the recovery rates of the targets are 90%‐99% in water samples when digested with β‐glucosidase for 90 mins before solid‐phase extraction, extracted with a Poly‐Sery HLB column, and washed with 15% methanol aqueous solution and 2% ammonia. The established instrument's limit of detection (LOD) was between 0.02 μg/L and 0.39 μg/L, and the limit of quantification (LOQ) was between 0.05 μg/L and 1.29 μg/L. Androstenedione, Boldenone, Methandienone, Nandrolone, and Testosterone were detected in all samples from the 2018 and 2022 wastewater influent and 2018 surface water, with concentrations of 3.06×101 ng/L‐1.33×103 ng/L, 1.03×102 ng/L‐8.15×102 ng/L, and 0.93×101 ng/L‐5.50×102 ng/L, respectively. The ecological risk of androgens in wastewater influent and surface water were medium to high and low to medium respectively. Moreover, the bio‐toxicity of androgens was predicted by the ECOSAR model, with Methandienone and Androstenedione having the highest and lowest acute and chronic toxicity, respectively. This study suggests that the risk of environmental androgens should not be ignored, and further research should be carried out.

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Exposure of adult zebrafish to androstenedione alters thyroid hormone levels and the transcriptional expression of genes related to the hypothalamus-pituitary-thyroid axis

Cited by 2 publications

References 39 publications

Toxic effects of bisphenol AF on the embryonic development of marine medaka (Oryzias melastigma)

Toxic effects of bisphenol AF on the embryonic development of marine medaka (Oryzias melastigma)

Trace Analysis of Five Androgens in Environmental Waters by Optimization of Enzymolysis and Solid‐Phase Extraction Ultra‐Performance Liquid Chromatography–Mass Spectrometry and its Risk Assessment

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