Exposure of bovine sperm to pro-oxidants impairs the developmental competence of the embryo after the first cleavage

Silva, Patrícia F.N.; Gadella, Bart M.; Colenbrander, B.; Roelen, Bernard A.J.

doi:10.1016/j.theriogenology.2006.09.032

Cited by 56 publications

(46 citation statements)

References 51 publications

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“…This finding is in accordance with that reported by Silva et al (2007) and supports the hypothesis that before the acrosome reaction, spermatozoa should undergo capacitation. Moreover, it is also possible that spermatozoa died before starting the acrosome reaction.…”

Section: Discussionsupporting

confidence: 93%

“…Exposure of spermatogenic cells to heat stress is known to produce reactive oxygen species (ROS; Ishii et al 2005;Nichi et al 2006). Because of excessive ROS production, the membrane loses its fluidity and structure, which has been related to poor sperm motility and function (Silva et al 2007;Du Plessis et al 2010). Similarly, in the present study we observed that heat stress significantly affects sperm plasma membrane integrity and MMP in a highly correlated manner (r ¼ 0.95).…”

Section: Discussionsupporting

confidence: 72%

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Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

Rahman

Vandaele²,

Rijsselaere

et al. 2014

Reprod. Fertil. Dev.

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Abstract. Heat stress has long been recognised as a cause of subfertility in farm animals. The objectives of the present study were to elucidate the effect of heat stress on sperm function and involvement of the mitogen-activated protein kinase (MAPK) 14 signalling pathway. Spermatozoa incubated for 4 h at a physiological temperature (38.58C) exhibited significantly (P , 0.05) reduced motility, plasma membrane integrity and mitochondrial potential compared with nonincubated spermatozoa; the reductions in these parameters were more severe following incubation at a hyperthermic (418C) temperature (P , 0.01). Percentages of fertilisation and embryo development were highly affected in spermatozoa incubated at 418C compared with non-incubated spermatozoa (P , 0.01). Similarly, embryo quality was adversely affected by sperm incubation at 418C, as indicated by a higher apoptotic cell ratio in Day 7 blastocysts compared with that in the non-incubated control group (14.6% vs 6.7%, respectively; P , 0.01). Using SB203580 (10 mg mL À1 ), a specific inhibitor of the p38 MAPK pathway, during sperm hyperthermia reduced MAPK14 activation (24.9% vs 35.6%), increased sperm motility (45.8% vs 26.5%) and reduced DNA fragmentation (16.9% vs 23.4%) compared with the untreated control group, but did not improve subsequent fertilisation and embryo development. In conclusion, heat stress significantly affects the potential of spermatozoa to penetrate oocytes, as well as subsequent embryo development and quality. Notably, the data show that the MAPK14 signalling pathway is largely involved in heat-induced sperm damage. However, further research is needed to elucidate other signalling pathways possibly involved in heat-induced sperm damage.Additional keywords: functional parameters, mitogen-activated protein kinase 14 phosphorylation, terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 72%

Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

Rahman

Vandaele²,

Rijsselaere

et al. 2014

Reprod. Fertil. Dev.

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“…The innate antioxidant defenses in embryos are not sufficient to counter the oxidative stress encountered during in vitro culture. Several studies have documented the damages caused by pro-oxidants and reactive oxygen species (ROS) on in vitro cultured murine [5], bovine [6] and porcine [7] gametes and embryos. Similar observations have also been made in human IVF study [8].…”

Section: Introductionmentioning

confidence: 99%

Effect of α-tocopherol supplementation on in vitro maturation of sheep oocytes and in vitro development of preimplantation sheep embryos to the blastocyst stage

Natarajan

Shankar²,

Deecaraman

2010

J Assist Reprod Genet

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Purpose To determine the effects of α-tocopherol supplementation to oocyte maturation media and embryo culture media on the yield of ovine embryos. Methods α-tocopherol, at concentrations of 0, 50, 100, 200, 400 and 500 µM was supplemented to ovine oocyte or embryo culture media and cultured at 5% or 20% O 2 levels. Percentages of cleavage, morula and blastocyst, total cell count and comet assay were taken as indicators of developmental competence of embryos. Results 200 µM α-tocopherol in embryo culture medium at 20% O 2 level significantly increased the rates of cleavage (P<0.05), morulae (P<0.05) and blastocyst (P<0.01) formation and blastocyst total cell number (P<0.01) when compared with control. The rates of blastocyst formation were also significantly higher in 100 µM (P<0.01) and 400 µM (P<0.05) supplemented groups than control. Conclusion α-tocopherol supplementation may enhance the in vitro developmental competence of ovine embryos by protecting them from oxidative damage.

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“…ROS and other noxious substances causes increased number of mitochondrial DNA mutations, nuclear DNA fragmentation, impair sperm membrane integrity and therefore motility and also affect the genetic constitution of the sperm [23,24]. Several studies showed that diminished DNA integrity in the sperm affects the developmental competence of the embryo [25,26]. DNA integrity is a prerequisite, not only for the fertilization of an oocyte, but also for oocyte activation and achievement of an ongoing pregnancy [27].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Efficacy of Low Dose and High Dose Antioxidants along with Antibiotics in Idiopathic Oligo, Astheno and Oligoasthenospermia

Bm¹,

Ehsan²,

Ehsan³

et al. 2015

Andrology

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Objective: The aim of this study was to explore the efficacy of low dose and high dose antioxidants for the treatment of idiopathic oligiospermia, asthenospermia and oligoasthenospermia. Materials and methods:This prospective quasi experimental study was conducted in Infertility Care and Research Center (ICRC) Ltd, Dhaka, Bangladesh between January 2013 and December 2014. Eighty four patients were the target population for this study. After thorough investigations those patients were diagnosed as idiopathic oligo, astheno and oligoasthenospermia were recruited for this study. Those patients whose female partner had infertility factor except PCOS were excluded from this study. For treatment patients were divided into two groups by lottery. Treatment for group A was Cap Doxycycline 100 mg twice daily for 1 month and tablet Oligocare, low dose antioxidant (Meyer Organic Pvt Ltd, India) 1 tab twice daily for 2 months. Group B was treated by Cap doxycycline 100 mg twice daily for 1 month and combination of micronutrients (High dose antioxidants) for 2 months. Ovulation induction was given to female partner of patient whose semen parameters improved. Results for pregnancy were observed for 6 ovulatory cycles. Data were expressed as mean standard deviation and percentage. Student's t test and χ 2 tests were done for test of significance where appropriate. A p value <0.05 was considered as significant. Results:In both treatment groups both count and motility were increased significantly after 2 months of treatment. Though mean semen parameter improved significantly in both treatment groups, there was significant difference in number of patient improvement between the groups. In high dose group 79% patients improved after two months of treatment whereas 48% patients improved in low dose group. Similarly pregnancy rate was also higher in high dose group 22% in comparison to 12% in low dose group. Conclusion:Antioxidants if can be used at a higher dose instead of low dose can give better result in terms of improvement of sperm count, motility and subsequently pregnancy rates.

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Exposure of bovine sperm to pro-oxidants impairs the developmental competence of the embryo after the first cleavage

Cited by 56 publications

References 51 publications

Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

Effect of α-tocopherol supplementation on in vitro maturation of sheep oocytes and in vitro development of preimplantation sheep embryos to the blastocyst stage

Comparison of Efficacy of Low Dose and High Dose Antioxidants along with Antibiotics in Idiopathic Oligo, Astheno and Oligoasthenospermia

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