Exposure of cultured human proximal tubular cells to cadmium, mercury, zinc and bismuth: toxicity and metallothionein induction

Rodilla, Vicente; Miles, Adrian T.; Jenner, William N.; Hawksworth, Gabrielle

doi:10.1016/s0009-2797(98)00059-3

Cited by 104 publications

(69 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[18][19][20][21] In fact, it has been reported that after its absorption Cd is taken up by the hepatocytes where it induces the biosynthesis of MTs. Cd ions are bound by MTs via thiol groups of cysteine residues leading to Cd-MT complexes; 2,3,[25][26][27][28][29] then from the liver the Cd ions circulate in blood bound to MTs until reaching the kidneys. 7,17,30 The Cd-MT complex is easily filtered through the glomerular membrane and taken up by renal tubular cells.…”

Section: Discussionmentioning

confidence: 99%

Nanotoxicological evaluation of oxidative responses in rat nephrocytes induced by cadmium

Trabelsi¹,

Azzouz²,

Ferchichi³

et al. 2013

IJN

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Nanotoxicological evaluation of oxidative responses in rat nephrocytes induced by cadmium

Trabelsi¹,

Azzouz²,

Ferchichi³

et al. 2013

IJN

View full text Add to dashboard Cite

“…Bismuth is an environmentally-friendly element, with very low toxicity, and a widespread pharmaceutical use [20,21]. The development and use of green electrode materials is extremely attractive for the routine use of disposable (one -shot) metal sensors.…”

Section: Background and Historymentioning

confidence: 99%

Stripping Analysis at Bismuth Electrodes: A Review

Wang¹

2005

Electroanalysis

566

386

View full text Add to dashboard Cite

For many years mercury electrodes were the transducer of choice in stripping voltammetry of trace metals owing to their high sensitivity, reproducibility, and renewability. However, because of the toxicity of mercury, alternative (environmentally friendly) electrode materials are highly desired for both centralized and field applications. Recently introduced bismuth electrodes offer a very attractive alternative to commonly used mercury electrodes. Such electrodes display well-defined, undistorted and highly reproducible response, favorable resolution of neighboring peaks, high hydrogen evolution, with good signal-to-background characteristics comparable to those of common mercury electrodes. The attractive stripping behavior of bismuth electrodes reflects the ability of bismuth to form fused multicomponent alloys with heavy metals. Bismuth stripping electrodes thus hold great promise for decentralized metal testing, with applications ranging from continuous remote sensing to single-use measurements. Fundamental studies aimed at understanding the behavior of bismuth film electrodes should lead to rational preparation and operation of reliable alternative (non -mercury) stripping electrodes that would have a major impact upon electroanalysis of trace metals. This article reviews the development, behavior, scope and prospects of bismuth electrodes for stripping-based electrochemical measurements of trace metals.

show abstract

“…Also, CsA-induced tubular toxicity is attributed to disturbed blood flow in the kidney (Busauschina et al, 2004). Zinc is not considered to be nephrotoxic in humans (Klaassen, 2004), or in cultured human proximal tubular cells (Rodilla et al, 1998). Therefore, DF, CsA and Zn were chosen on the supposition that they would not be directly toxic to cultured tubular cells in the same setting as direct toxicants.…”

Section: Introductionmentioning

confidence: 99%

In vitro gene expression analysis of nephrotoxic drugs in rat primary renal cortical tubular cells

Suzuki¹,

Inoue²,

Matsushita³

et al. 2008

J of Applied Toxicology

View full text Add to dashboard Cite

Rat primary renal cortical tubular cells were exposed to seven test substances, some with, and some without, known direct renal tubular cell toxicity. Cells were exposed to the substances at either one-third or one-tenth of the TC50 for cytotoxicity for 6 h or 24 h, so as not to induce cytotoxicity but to cause some transcriptional changes. Transcriptional profiles were investigated by using the Affymetrix Rat Toxicology U34 arrays, containing probes for more than 850 genes and ESTs. Four direct toxicants, cisplatin (CDDP), its less nephrotoxic analogue carboplatin (CBDCA), cephaloridine and gentamicin, were grouped together in a hierarchical clustering. In addition, the four direct toxicants affected more than 32 transcripts at their subcytotoxic concentrations at either 6 h or 24 h exposure. On the other hand, diclofenac, cyclosporine A and zinc, which are not considered to be directly toxic to tubules, affected less than 12 transcripts. Decreased Map3k12 and increased Hmox1 were commonly observed among the four direct toxicants, which appeared to be responses to cellular damage. Two platinum complexes, CDDP and CBDCA, induced similar changes, regardless of exposure duration or concentration. The types of transcriptional changes observed in this study were consistent with previously reported in vivo data, although there were some differences. These observations suggest that an in vitro gene expression analysis approach using GeneChip is feasible for screening for direct tubular toxicity of drugs and may help to clarify the underlying mechanisms of tubular toxicity.

show abstract

Exposure of cultured human proximal tubular cells to cadmium, mercury, zinc and bismuth: toxicity and metallothionein induction

Cited by 104 publications

References 46 publications

Nanotoxicological evaluation of oxidative responses in rat nephrocytes induced by cadmium

Nanotoxicological evaluation of oxidative responses in rat nephrocytes induced by cadmium

Stripping Analysis at Bismuth Electrodes: A Review

In vitro gene expression analysis of nephrotoxic drugs in rat primary renal cortical tubular cells

Contact Info

Product

Resources

About