Exposure of pregnant rats to staphylococcal enterotoxin B increases offspring splenic Treg number and function via decreasing FoxP3 methylation

SERS-Active Composites with Au–Ag Janus Nanoparticles/Perovskite in Immunoassays for Staphylococcus aureus Enterotoxins

“…The length of Au−Ag Janus NPs was around 84.4 ± 4.2 nm and the size of the formed Ag islands was about 53.2 ± 2.7 nm (Figure S2). By controlling the concentration of AgNO 3 solution, a series of Au−Ag Janus NPs with different Ag island sizes, including Au−Ag Janus NPs (1) , Au−Ag Janus NPs (2) , Au−Ag Janus NPs (3) , Au−Ag Janus NPs (4) , and Au−Ag Janus NPs (5) , could be synthesized using 20, 40, 60, 100, and 120 μL AgNO 3 solution, respectively. As displayed in Figure S3A, for Au−Ag Janus NPs (1) , Ag structures were hardly observed on the Au NPs' surface.…”

“…Staphylococcus aureus enterotoxins (SEs) were produced by S. aureus at 20–37 °C and a suitable pH in food, which was the main pathogenic factor of food-borne diseases. , Epidemiological studies had shown that SEs causing food-borne diseases were mainly consisted of S. aureus enterotoxins A, B, C, D, E (SEA, SEB, SEC, SED, SEE) .…”

“…6−10 Alternatively, SERS biosensors possessed high sensitivity down to single-molecule detection and showed potential for application in biological molecule analysis, 11−15 environmental monitoring, 16,17 and disease diagnosis, 18−24 and would be a promising tool for reliable SE detection. However, there were still some issues to be solved for SERS signal-dependent sensitive and accurate SE detection: (1) the high and non-interference SERS signals for ultrasensitive and precise detection; (2) the uniform position of Raman labels on the surface of SERS for the production of reproducible SERS signals. It is vital to fabricate strong and stable SERS-active nanostructures for precise SE detection.…”

“…35 For the growth of the Ag islands, an aliquot of 1 mL of 2 nM Au NPs and 20 μL of 1 mM MBIA were mixed. After incubation at 60 °C for 2 h, HQ (60 μL, 10 mM) and AgNO 3 (1 mM) were added under vigorous stirring into the above solution for 5 h. When 18, 35.7, 52.6, 84.7, and 129 μM of AgNO 3 solutions were applied, a series of Au−Ag Janus NPs (Au−Ag Janus NPs (1) , Au−Ag Janus NPs (2) , Au−Ag Janus NPs (3) , Au−Ag Janus NPs (4) , and Au−Ag Janus NPs (5) ) were prepared. After the reaction, the NPs were obtained by centrifugation and dispersed evenly in 500 μL deionized water.…”