A b s t r a c t. The effects of magnetic field treatments of French marigold seeds on germination, early seedling growth and biochemical changes of seedlings were studied under controlled conditions. For this purpose, seeds were exposed to five different magnetic seed treatments for 3 min each. Most of seed treatments resulted in improved germination speed and spread, root and shoot length, seed soluble sugars and a-amylase activity. Magnetic seed treatment with 100 mT maximally improved germination, seedling vigour and starch metabolism as compared to control and other seed treatments. In emergence experiment, higher emergence percentage (4-fold), emergence index (5-fold) and vigorous seedling growth were obtained in seeds treated with 100 mT. Overall, the enhancement of marigold seeds by magnetic seed treatment with 100 mT could be related to enhanced starch metabolism. The results suggest that magnetic field treatments of French marigold seeds have the potential to enhance germination, early growth and biochemical parameters of seedlings.K e y w o r d s: marigold, seed, stimulation, magnetic field
INTRODUCTIONMarigold is grown as an ornamental crop for loose flowers and as bedding plant in landscape. French marigold (Tagetes patula L.) is ideal for rockeries, edging, hanging baskets and window boxes. Both leaves and flowers are equally important from the medicinal point of view. Marigold petals are commercially valuable as a natural source of xanthophylls, pigments used primarily in poultry industry to intensify yellow colour of egg yolk. The essential oil present in different species of Tagetes is largely used in compounding of high-grade perfumes (Kaul et al., 2000). Crop stand establishment is always an important consideration in commercial field production. Similarly, rapid and uniform seedling emergence is the demand of every farmer as it is the foundation on which stand establishment is based and potential yield is determined. But, the emergence and stand establishment of marigold seeds are often slow and extremely erratic, particularly under hot or cool field conditions (Afzal et al., 2009). Currently, direct seeding is also popular among the vegetable and flower farming community in the world. However, it often results in uneven germination, slow emergence and poor stand establishment, but is less expensive initially and widely used in field operations. One of the greatest problems for marigold production is poor plant stand establishment. Magnetic seed pretreatments have been used successfully to improve germination rate and crop establishment, boost growth, development and ultimately yield of many horticultural and agronomic crops (Aladjadjiyan, 2010;Moon and Chung, 2000;Pietruszewski and Kania, 2010). Magnetic field pretreatment not only increased seed germination rate, seedling growth and yield (Balouchi and Sanavy, 2009) but also reduced the attack of pathogenic diseases (De Souza et al., 2006;Yinan et al., 2005). The beneficial effect of magnetic seed stimulation has been associated with various...