Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation

Abstract

The impact of nanotechnology in the field of agricultural sciences creates the need to study in greater detail the effect of products offering nanoparticles for application in plant species of agricultural interest. The objective of this study was to determine the response of stevia ( Stevia rebaudiana B.) in vitro to different concentrations of AgNPs (silver nanoparticles), as well as to characterize and identify their absorption, translocation and accumulation mechanisms. Nodal segments of stevia grown in MS medium supplemented with AgNPs (0,12.5, 25, 50,100 and 200 mg L −1 ) were used. After 30 days of in vitro shoot proliferation, the number of shoots per explant, shoot length, chlorophyll content, dry matter content and the metallic silver (Ag) content of the plants were quantified. In addition, characterization, transport and accumulation of silver nanoparticles were performed by microscopic analysis. AgNPs were shown to be present in epidermal stem cells, within vascular bundles and in intermembrane spaces. In leaves, they were observed in ribs and stomata. The current and future use of AgNPs in agricultural sciences opens up the possibility of studying their effects on different plant species.

Biosynthesis and characterization of silver nanoparticles using Ochradenus arabicus and their physiological effect on Maerua oblongifolia raised in vitro

“…TEM was used to characterize the shape and size of AgNPs that were synthesized by the reduction of Ag + using O. arabicus leaf extract. The TEM images obviously showed that the synthesized AgNPs were spherical, in line with previous results 26,27 . The dispersion properties of the spherical particles can vary as per their size, exact shape, and composition 28 .…”

“…In general, the application of AgNPs reportedly increases the photosynthetic pigment amount in vanilla 32 and sugarcane 33 . The reason for the increased chlorophyll content in our study could be the increases in nitrogen, magnesium, and iron concentrations in the plant tissues treated with AgNPs, given that these elements are associated with chlorophyll biosynthesis 26 . In contrast, decreased in chlorophyll content were also reported in Arabidopsis thaliana and rice 34,35 .…”

“…The response of the chlorophyll content (chl a and chl b) to AgNP treatment in the present study correlates with a previous report 29 in which the total chl in wheat grown in vitro was significantly promoted after treatment with 25 mg L −1 AgNPs. Further, another study showed a remarkable increase in the chlorophyll content in Stevia rebaudiana after treatment with 25 mg L −1 AgNPs 26 . In general, the application of AgNPs reportedly increases the photosynthetic pigment amount in vanilla 32 and sugarcane 33 .…”

Argovit™ Silver Nanoparticles Effects on Allium cepa: Plant Growth Promotion without Cyto Genotoxic Damage

“…For onions, cytotoxic damage apparently begins with 100 µg/mL of AgNPs because only a small increase of malondialdehyde is observed ( Figure 5 c). While on the other plants, with this concentration, the damage is quite evident not only at the molecular level but also physiologically, due to different antioxidant response of these plants [ 50 , 62 , 63 ].…”

Post-synthesis nanostructuration of BSA-Capsaicin nanoparticles generated by sucrose excipient

“…The experimental preparation of the samples and operating conditions of the microscope were similar to those previously reported by Sánchez-Segura et al 14 , Sánchez-Arreguin et al 10 and modified by Castro-González et al . 40 . On the other hand, the morphometric parameters were calculated with equations proposed by Syverud et al 41 .…”