Exposure Time Related Oxidative Action of Hyperbaric Oxygen in Rat Brain

Abstract

Hyperbaric oxygen (HBO) is known to cause oxidative stress in several organs and tissues. Due to its high rate of blood flow and oxygen consumption, the brain is one of the most sensitive organs to this effect. The present study was performed to elucidate the relation of HBO exposure time to its oxidative effects in rats' brain cortex tissue. For this purpose, 49 rats were randomly divided into five groups. Except the control group, study groups were subjected to three atmospheres HBO for 30, 60, 90, and 120 min. Their cerebral cortex layer was taken immediately after exposure and used for analysis. Thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and nitrate-nitrite (NOX) levels were determined. TBARS and SOD levels were found to increase in a time-dependent manner. GSH-Px activity reflected an inconsistent course. NOX levels were found to be increased only in the 120 min exposed group. The results of this study suggests that HBO induced oxidative effects are strongly related with exposure time.

Effect of Preconditioned Hyperbaric Oxygen and Ozone on Ischemia-Reperfusion Induced Tourniquet in Skeletal Bone of Rats

“…In addition, Ay et al [4] also reported in another study that the oxidative effect of 2 h of HBO exposure declines to physiological levels within 90 min at most for erythrocytes and in lung tissue in rats. Korkmaz et al [40]. MDA and SOD levels were found to increase in a timedependent HBO exposure, but GSH-Px activity reflected an inconsistent course in rat brain.…”

Hyperbaric oxygen therapy reduces the severity of necrotizing enterocolitis in a neonatal rat model

“…It is well known that HBO stimulates or upregulates some biological processes such as angiogenesis, collagen synthesis, leukocyte adhesion, erythropoietin, and release of vascular endothelial growth factor (VGEF), which provide tissue healing [8,9,26]. Hyperbaric oxygen is also known for producing oxidative stress in an appropriate exposure pressure and duration-dependent manner [27,28], and it is suggested that this oxidative stress may be another of its beneficial mechanisms via stimulating antioxidant enzymes.…”

The Neuroprotective Effect of Curcumin and Nigella sativa Oil Against Oxidative Stress in the Pilocarpine Model of Epilepsy: A Comparison with Valproate

“…The animal brain is often said to be especially sensitive to oxidative damage [5]. This may be attributed to its high oxygen consumption, the large quantities of oxidizable lipids and metals, and the comparatively less antioxidant mechanisms [6,7].…”