Exposure to 50 Hz electromagnetic fields enhances hair follicle regrowth in C57BL/6 mice

Abstract

Many studies have suggested that electromagnetic field activity affects the cellular activity of many types of cells involved in forming hair follicles. However, the bio-effects of electromagnetic fields on hair follicle growth have not been fully elucidated. This present study was designed to determine whether 50 Hz electromagnetic fields increased hair follicle regrowth. In this experiment, C57BL/6 mice were used to present the model of depilation-induced hair follicle cycling, and then those mice were divided at random into the control group and the electromagnetic field group. After electromagnetic field (50 Hz, 5 mT) exposure for 16 days, the skin specimens of the mice were harvested to assess for hair regrowth, and epidermal stem cells proliferation was evaluated by immunofluorescence staining. The expression and location of keratinocyte growth factors were also tested. Our results showed that, compared to the control, the hair club formed faster on the 3rd day, and most of the hair shafts erupted earlier from the pore in the epidermis on the 9th day after depilation, and the hairs length was significantly longer on the 16th day within the electromagnetic field group. After electromagnetic field treatment, there were more Ki67+ cells in the outer root sheath and hair bulb where it co-localized with K15+ cells compared to the control. Keratinocyte growth factors were expressed in the inner root sheath in both groups, and the electromagnetic field group showed more expression of keratinocyte growth factors. Our data suggested that the hair-growth-promoting effect of the 50 Hz electromagnetic field was observed in depilation-induced hair follicles cycling, which was associated with 50 Hz electromagnetic field enhancing K15+ stem cells proliferation and increased keratinocyte growth factor expression. Impact statement In this study, our experiments confirmed that 50 Hz EMF affected hair follicle regrowth, and 50 Hz EMF enhanced K15+ stem cells proliferation in the hair bulb and follicular outer root sheath of hair follicles. Those results indicated that 50 Hz EMF may be beneficial for functional healing of hair loss.

Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose

“…KGF gene, also known as FGF7 , belongs to the FGFs gene family and is an epithelial-specific growth factor that can specifically stimulate the proliferation of epithelial cells such as keratinocytes [ 20 ]. Li [ 21 ] found that the KGF gene exists in the inner root sheath of mouse feather follicles and may play a regulatory role. Since the stromal cells of the inner root sheath produce the feather shaft and KGF receptors are present on the stromal cells, it is speculated that KGF may promote feather growth.…”

The efficacy and safety of the combination of photobiomodulation therapy and pulsed electromagnetic field therapy on androgenetic alopecia

“…Low‐frequency electromagnetic fields facilitate the generation of bio‐engineered hair follicles 26 and increase the expression of anagen‐related molecules in human dermal papilla cells 8 . Exposure to electromagnetic fields enhances hair club formation via increased proliferation of epidermal stem cells from the bulge region and facilitates the development of a new lower follicle 9 . Motion‐activated and wearable electric stimulation devices effectively accelerate hair growth and promote hair follicle formation 27 …”

“…It enhances the activation and proliferation of dermal papilla cells in the human hair follicle 8 . Xinping Li et al found that treatment with 50 Hz electromagnetic field leads to an increase in hair length in hair follicle cycling models 9 . Clinically, the use of electromagnetic pulses to treat AGA was corroborated by Maddin, 10,11 who showed that PEMF stimulated hair growth.…”

Is the Technology Era Aging You? A Review of the Physiologic and Psychologic Toll of Technology Use

“…Interestingly, lower frequencies, such as ELFs, may promote hair growth, thus emphasizing the importance of dose, frequency, and strength on the impact of electromagnetic radiation on the body. 28 A meta-analysis was performed of the in vitro effects of RF-EM on human and animal cells. Of the 746 human cell experiments, 45.3% of studies demonstrated a significant effect of RF energy on cells, whereas 54.7% did not (p 5 .001).…”