Exposure to an Androgenic Agricultural Pollutant Does Not Alter Metabolic Rate, Behaviour, or Morphology of Tadpoles

Martin, Jake M.; Orford, Jack T.; Melo, Gabriela C.; Tan, Hung; Mason, Rachel T.; Ozeki, Shiho; Wong, Bob B. M.; Alton, Lesley A.

doi:10.2139/ssrn.3965235

Cited by 2 publications

(3 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Water changes (6 L, i.e., 33%) were performed once a week. To ensure consistent conditions across tanks, weekly temperature (18.56 ± 0.19 °C, mean ± SD; n = 96) and pH (6.94 ± 0.13; n = 96) checks were performed (data available at Martin et al, 2023). Tadpole survival in each treatment tank was measured at the end of the 1-month exposure and did not differ significantly among treatments (mean ± SD survival percentage 82.9 ± 17.3%; see Supporting Information S1 for survival by treatment comparisons).…”

Section: Experimental Exposurementioning

confidence: 99%

Impacts of Exposure to Ultraviolet Radiation and an Agricultural Pollutant on Morphology and Behavior of Tadpoles (Limnodynastes tasmaniensis)

Orford,

Tan,

Martin

et al. 2024

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Amphibians are the most threatened vertebrate class globally. Multiple factors have been implicated in their global decline, and it has been hypothesized that interactions between stressors may be a major cause. Increased ultraviolet (UV) radiation, as a result of ozone depletion, has been identified as one such stressor. Exposure to UV radiation has been shown to have detrimental effects on amphibians and can exacerbate the effects of other stressors, such as chemical pollutants. Chemical pollution has likewise been recognized as a major factor contributing to amphibian declines, particularly, endocrine‐disrupting chemicals. In this regard, 17β‐trenbolone is a potent anabolic steroid used in the agricultural industry to increase muscle mass in cattle and has been repeatedly detected in the environment where amphibians live and breed. At high concentrations, 17β‐trenbolone has been shown to impact amphibian survival and gonadal development. In the present study, we investigated the effects of environmentally realistic UV radiation and 17β‐trenbolone exposure, both in isolation and in combination, on the morphology and behavior of tadpoles (Limnodynastes tasmaniensis). We found that neither stressor in isolation affected tadpoles, nor did we find any interactive effects. The results from our 17β‐trenbolone treatment are consistent with recent research suggesting that, at environmentally realistic concentrations, tadpoles may be less vulnerable to this pollutant compared to other vertebrate classes. The absence of UV radiation–induced effects found in the present study could be due to species‐specific variation in susceptibility, as well as the dosage utilized. We suggest that future research should incorporate long‐term studies with multiple stressors to accurately identify the threats to, and subsequent consequences for, amphibians under natural conditions. Environ Toxicol Chem 2024;00:1–12. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

show abstract

Section: Experimental Exposurementioning

confidence: 99%

Impacts of Exposure to Ultraviolet Radiation and an Agricultural Pollutant on Morphology and Behavior of Tadpoles (Limnodynastes tasmaniensis)

Orford,

Tan,

Martin

et al. 2024

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…33%) were performed once a week. To ensure consistent conditions across tanks, weekly temperature (18.56 ± 0.19 °C, mean ± SD; n = 96) and pH (6.94 ± 0.13; n = 96) checks were performed (data available at Martin et al 2023). Tadpole survival in each treatment tank was measured at the end of the one-month exposure and did not differ significantly among treatments (mean ± SD survival percentage: 82.9 ± 17.3 %; see Supplementary material for survival by treatment comparisons).…”

Section: Experimental Exposurementioning

confidence: 99%

“…All of the data and code associated with this manuscript are available from the Open Science Framework (Martin et al 2023; https://doi.org/10.17605/OSF.IO/XYG63)…”

Section: Data Availability Statementmentioning

confidence: 99%

Impacts of exposure to UV radiation and an agricultural pollutant on morphology and behaviour of tadpoles (Limnodynastes tasmaniensis)

Orford,

Tan,

Martin

et al. 2023

Preprint

View full text Add to dashboard Cite

Amphibians are the most threatened vertebrate class globally, with many species at risk of extinction. Multiple factors have been implicated in the global decline of amphibian populations, and it has been hypothesised that interactions between these stressors may be responsible for such rapid declines. Increased ultraviolet (UV) radiation as a result of ozone depletion has been identified as one such stressor. Exposure to UV radiation has been shown to have detrimental effects on amphibian survival, development, and behaviour. UV radiation has also been shown to exacerbate the effects of other stressors, such as chemical pollutants. Chemical pollution has been recognised as another major factor contributing to amphibian declines, particularly, endocrine-disrupting chemicals. 17β-trenbolone is a potent anabolic steroid used in the agricultural industry to increase muscle mass in cattle, and has been repeatedly detected in the environment where amphibians live and breed. At high concentrations, 17β-trenbolone exposure has been shown to impact amphibian survival and gonadal development. In the present study, we investigated the effects of one-month exposure to environmentally realistic levels of UV radiation and 17β-trenbolone, both in isolation and in combination, on the morphology and behaviour of spotted marsh frog tadpoles (Limnodynastes tasmaniensis). We found that neither stressor in isolation affected the morphology or behaviour of tadpoles, nor did we find any interactive effects. The results from our 17β-trenbolone treatment are consistent with recent research suggesting that, at environmentally realistic concentrations, tadpoles may be less vulnerable to this pollutant compared to other vertebrate classes (e.g. fish). The absence of UV radiation-induced effects found in this study could be due to species-specific variation in susceptibility to UV radiation, as well as the UV dosage utilised in the present study. We suggest future research incorporates long-term studies using multiple stressors at environmentally realistic levels to accurately identify the threats, and subsequent consequences, that amphibians face in natural conditions.

show abstract

Exposure to an Androgenic Agricultural Pollutant Does Not Alter Metabolic Rate, Behaviour, or Morphology of Tadpoles

Cited by 2 publications

References 53 publications

Impacts of Exposure to Ultraviolet Radiation and an Agricultural Pollutant on Morphology and Behavior of Tadpoles (Limnodynastes tasmaniensis)

Impacts of Exposure to Ultraviolet Radiation and an Agricultural Pollutant on Morphology and Behavior of Tadpoles (Limnodynastes tasmaniensis)

Impacts of exposure to UV radiation and an agricultural pollutant on morphology and behaviour of tadpoles (Limnodynastes tasmaniensis)

Contact Info

Product

Resources

About