Exposure to epididymal extracellular vesicles enhances immature sperm function and sustains vitality of cryopreserved spermatozoa in the domestic cat model

Rowlison, Tricia; Ottinger, Mary Ann; Comizzoli, Pierre

doi:10.1007/s10815-021-02214-0

Cited by 10 publications

(15 citation statements)

References 54 publications

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“…The incorporation took place at the shortest incubation time (1 h) in the sperm midpiece. Our results are in line with a previous report showing optimal incorporation in cat spermatozoa co-incubated with oviductal or epididymal EVs for 1 h (Ferraz et al 2019, Rowlison et al 2021, while male gametes from mouse and pig models required a co-incubation time greater than two hours (Al-Dossary et al 2013, Alcântara-Neto et al 2020. This suggests that the binding of the EVs with the spermatozoa could depend on the source of the spermatozoa, the kind of EVs, the concentration and the experimental conditions in which the co-incubation was carried out.…”

Section: Discussionsupporting

confidence: 92%

“…Epididymosomes are released from the epididymal epithelium by apocrine secretion into the lumen, and they were first documented in hamsters but have since been identified in all mammals studied, including mice, rats, bulls, rams, and humans ( Sullivan et al 2013 ). The epididymosomes can interact with transiting spermatozoa and deliver cargo that influences not only sperm maturation but also capacitation, acrosome reaction, fertilization, embryonic development, and long-term health of the offspring ( Ellerman et al 2006 , Sullivan et al 2013 , Martin-DeLeon et al 2015 , Saewu et al 2017 , Nixon et al 2019 , Gaikwad et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Extracellular vesicles from seminal plasma to improve fertilizing capacity of bulls

Consiglio

Capra

Monferrini

et al. 2022

Reproduction and Fertility

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Seminal plasma contains extracellular vesicles (EVs) that vehicle RNA, proteins, and other molecules able to influence the biological function of sperm. The aim of this study was to improve the fertilizing capacity of male gametes of low-fertility bulls using EVs isolated by ultracentrifugation from the seminal plasma of a bull of proven fertility. After dose-response curve, 10×106 sperm of low-fertility bulls were co-incubated for an hour with 400×106 EVs/ml. In addition, it has been verified that the incorporation of EVs, which takes place in the sperm midpiece, is maintained for 5 hours and even after cryopreservation. Subsequently, the spermatozoa of low-fertility bulls, with EVs incorporated, were used for the in vitro production of embryos. The rate of blastocyst at seventh day yield in vitro, with the use of sperm with EVs incorporated, increased by about twice the yield obtained with the same sperm in the absence of EVs: bulls having an average embryonic yield of 6.41±1.48%, 10.32±4.34% and 10.92±0.95% improved their yield to 21.21±1.99%, 22.17±6.09% and 19.99±5.78%, respectively (P<0.05). These encouraging results suggest that it might be possible to keep breeding bulls with poor fertility. Further studies will be needed to evaluate the in vivo fertility of sperm treated with EVs and understand how the content of EVs is involve in the sperm-vesicle interaction and in the improved sperm performance.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Extracellular vesicles from seminal plasma to improve fertilizing capacity of bulls

Consiglio

Capra

Monferrini

et al. 2022

Reproduction and Fertility

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“…Sperm intracellular pH also regulates specific calcium channel activity in the flagellum (CatSper), which is further involved in processes such as sperm hyperactivation, energization, and acrosome reaction (Darszon et al, 2005;Darszon et al, 2011). Additionally, intracellular pH is directly related to sperm viability, with higher values of sperm kinetic parameters, membrane integrity, and mitochondrial activity at pH 7 and 7.5 being observed (Jones and Bavister, 2000;Rizvi et al, 2009;Contri et al, 2013). The external pH of the sperm directly influences the pH of the sperm cell's cytoplasm, which is linearly related to the extracellular pH (Mishra et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Effects of Diluent pH on Enrichment and Performance of Dairy Goat X/Y Sperm

Huang

et al. 2021

Front. Cell Dev. Biol.

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In this paper, on the basis of the differences in the hydrogen ion concentration (pH) of the diluent dairy goat semen on X/Y sperm motility, an X/Y sperm enrichment study was conducted to establish a simple and effective method for gender control in dairy goats. Dairy goat semen was diluted using different pH dilutions and was incubated. Then, the X/Y sperm ratio in the isolated upper sperm was determined using the double TaqMan qPCR method. The internal pH change pattern of sperm cells at different pH dilutions was measured using BCECF-AM probe, and the functional parameters of the isolated sperm were tested with the corresponding kit. Next, an in vitro fertilization test was conducted using isolated spermatozoa and oocytes to determine their fertilization rates, the percentages of female embryos, and the expression of genes related to developing potentially fertilized embryos. Results showed that the percentages of the X sperm cells in the upper sperm layer were 67.24% ± 2.61% at sperm dilution pH of 6.2 and 30.45% ± 1.03% at sperm dilution pH of 7.4, which was significantly different from 52.35% ± 1.72% of the control group (pH 6.8) (P < 0.01). Results also showed that there is a relationship between the external pHo and internal pHi of sperm cells. Furthermore, the percentages of female embryos after the in vitro fertilization of the isolated upper sperm with mature oocytes at pH 6.2 and 7.4 were 66.67% ± 0.05 and 29.73% ± 0.04%, respectively, compared with 48.57% ± 0.02% in the control group (pH 6.8). Highly significant differences occurred between groups (P < 0.01). Additionally, no significant difference was observed during the expression of genes related to embryonic development between the blastocysts formed from sperm isolated by changing the pH of the diluent and the control sperm (P > 0.05). Therefore, this study successfully established a simple and effective method for enriched X/Y sperms from dairy goats, which is important for regulating the desired sex progeny during dairy goat breeding and for guiding dairy goat production.

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“…Seminal plasma contains EVs produced by the prostate, epididymis, and testis, and healthy EVs have been shown to promote sperm-egg binding. Cat SP-EVs have been shown to improve the fertilization ability and developmental potential of immature spermatozoa, as well as spermatozoa motility after freezing and thawing ( Rowlison et al, 2021 ). SP-EVs have been shown to improve sperm motility after sperm culture, which may be due to the fact that SP-EVs contain many proteins that promote sperm survival and capacitance ( Mahdavinezhad et al, 2022 ).…”

Section: The Role Of Evs In Gametes Fertilizationmentioning

confidence: 99%

Messenger roles of extracellular vesicles during fertilization of gametes, development and implantation: Recent advances

Fan

Wang

et al. 2023

Front. Cell Dev. Biol.

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Extracellular vesicles (EVs) have become a research hotspot in recent years because they act as messengers between cells in the physiological and pathological processes of the human body. It can be produced by the follicle, prostate, embryo, uterus, and oviduct in the reproductive field and exists in the extracellular environment as follicular fluid, semen, uterine cavity fluid, and oviduct fluid. Because extracellular vesicles are more stable at transmitting information, it allows all cells involved in the physiological processes of embryo formation, development, and implantation to communicate with one another. Extracellular vesicles carried miRNAs and proteins as mail, and when the messenger delivers the mail to the recipient cell, the recipient cell undergoes a series of changes. Current research begins with intercepting and decoding the information carried by extracellular vesicles. This information may help us gain a better understanding of the secrets of reproduction, as well as assist reproductive technology as an emerging marker and treatment.

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Exposure to epididymal extracellular vesicles enhances immature sperm function and sustains vitality of cryopreserved spermatozoa in the domestic cat model

Cited by 10 publications

References 54 publications

Extracellular vesicles from seminal plasma to improve fertilizing capacity of bulls

Extracellular vesicles from seminal plasma to improve fertilizing capacity of bulls

Effects of Diluent pH on Enrichment and Performance of Dairy Goat X/Y Sperm

Messenger roles of extracellular vesicles during fertilization of gametes, development and implantation: Recent advances

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