Exposure to high nitrogen triggered a genotype‐dependent modulation of cell and root hydraulics, which can involve aquaporin regulation

Pou, Alícia; Hachez, Charles; Couvreur, Valentin; Maistriaux, Laurie; Ismail, Ahmed; Chaumont, François

doi:10.1111/ppl.13640

Cited by 6 publications

(3 citation statements)

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“…The high correlation between NO 3 uptake and the hydraulic response in the root system was previously described in several plants (Goŕska et al, 2010), further suggesting that the differences in NUE performances between RO and UC82 might also be derived from a different regulation of genes involved in water transport. A genotype-specific hydraulic response to NO 3 -, putatively derived from different aquaporin protein levels, was recently detected in maize roots (Pou et al, 2022).…”

Section: Co-expression Network Analysis Reveals N Responsive Modulesmentioning

confidence: 98%

Short-term transcriptomic analysis at organ scale reveals candidate genes involved in low N responses in NUE-contrasting tomato genotypes

Sunseri

Aci²,

Mauceri³

et al. 2023

Front. Plant Sci.

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BackgroundUnderstanding the complex regulatory network underlying plant nitrogen (N) responses associated with high Nitrogen Use Efficiency (NUE) is one of the main challenges for sustainable cropping systems. Nitrate (NO3-), acting as both an N source and a signal molecule, provokes very fast transcriptome reprogramming, allowing plants to adapt to its availability. These changes are genotype- and tissue-specific; thus, the comparison between contrasting genotypes is crucial to uncovering high NUE mechanisms.MethodsHere, we compared, for the first time, the spatio-temporal transcriptome changes in both root and shoot of two NUE contrasting tomato genotypes, Regina Ostuni (high-NUE) and UC82 (low-NUE), in response to short-term (within 24 h) low (LN) and high (HN) NO3- resupply. ResultsUsing time-series transcriptome data (0, 8, and 24 h), we identified 395 and 482 N-responsive genes differentially expressed (DEGs) between RO and UC82 in shoot and root, respectively. Protein kinase signaling plant hormone signal transduction, and phenylpropanoid biosynthesis were the main enriched metabolic pathways in shoot and root, respectively, and were upregulated in RO compared to UC82. Interestingly, several N transporters belonging to NRT and NPF families, such as NRT2.3, NRT2.4, NPF1.2, and NPF8.3, were found differentially expressed between RO and UC82 genotypes, which might explain the contrasting NUE performances. Transcription factors (TFs) belonging to several families, such as ERF, LOB, GLK, NFYB, ARF, Zinc-finger, and MYB, were differentially expressed between genotypes in response to LN. A complementary Weighted Gene Co-expression Network Analysis (WGCNA) allowed the identification of LN-responsive co-expression modules in RO shoot and root. The regulatory network analysis revealed candidate genes that might have key functions in short-term LN regulation. In particular, an asparagine synthetase (ASNS), a CBL-interacting serine/threonine-protein kinase 1 (CIPK1), a cytokinin riboside 5’-monophosphate phosphoribohydrolase (LOG8), a glycosyltransferase (UGT73C4), and an ERF2 were identified in the shoot, while an LRR receptor-like serine/threonine-protein kinase (FEI1) and two TFs NF-YB5 and LOB37 were identified in the root. DiscussionOur results revealed potential candidate genes that independently and/or concurrently may regulate short-term low-N response, suggesting a key role played by cytokinin and ROS balancing in early LN regulation mechanisms adopted by the N-use efficient genotype RO.

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Section: Co-expression Network Analysis Reveals N Responsive Modulesmentioning

confidence: 98%

Short-term transcriptomic analysis at organ scale reveals candidate genes involved in low N responses in NUE-contrasting tomato genotypes

Sunseri

Aci²,

Mauceri³

et al. 2023

Front. Plant Sci.

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“…For example, stomatal aperture is well studied and responds to both hydraulic and chemical signals from root to shoot, with osmotic adaptations to water deficit (Larcher 2003;Christmann et al 2007;Dodd et al 2010;Vandeleur et al 2014). Similarly, the dynamics of root hydraulic conductivity show short-term responses to the availability of water (Hachez et al 2012) through circadian rhythms (Caldeira et al 2014), but also soluble nutrient concentration, such as nitrate (Gorska et al 2008;Ishikawa-Sakurai et al 2014), possibly via aquaporin regulation (Javot & Maurel 2002;Pou et al 2022). Last, but not least, plant hydraulic properties are spatially heterogeneous and dynamic in leaves (Tardieu et al 2015;Earles et al 2018) and stems (Bohrer et al 2005;Couvreur et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…2014), possibly via aquaporin regulation (Javot & Maurel 2002; Pou et al . 2022). Last, but not least, plant hydraulic properties are spatially heterogeneous and dynamic in leaves (Tardieu et al .…”

Section: Introductionmentioning

confidence: 99%

Plant water uptake modelling: added value of cross‐disciplinary approaches

et al. 2022

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In recent years, research interest in plant water uptake strategies has rapidly increased in many disciplines, such as hydrology, plant ecology and ecophysiology. Quantitative modelling approaches to estimate plant water uptake and spatiotemporal dynamics have significantly advanced through different disciplines across scales. Despite this progress, major limitations, for example, predicting plant water uptake under drought or drought impact at large scales, remain. These are less attributed to limitations in process understanding, but rather to a lack of implementation of cross-disciplinary insights into plant water uptake model structure. The main goal of this review is to highlight how the four dominant model approaches, that is, Feddes approach, hydrodynamic approach, optimality and statistical approaches, can be and have been used to create interdisciplinary hybrid models enabling a holistic system understanding that, among other things, embeds plant water uptake plasticity into a broader conceptual view of soil-plant feedbacks of water, nutrient and carbon cycling, or reflects observed drought responses of plant-soil feedbacks and their dynamics under, that is, drought. Specifically, we provide examples of how integration of Bayesian and hydrodynamic approaches might overcome challenges in interpreting plant water uptake related to different travel and residence times of different plant water sources or trade-offs between root system optimization to forage for water and nutrients during different seasons and phenological stages.

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pH Stabilized NH4+-Fed Nutrition Promotes Higher B Uptake and Plant Growth in Rapeseed (Brassica napus L.) by the Upregulation of B Transporters

Chowdhury,

Hossain,

Sagervanshi

et al. 2024

J Plant Growth Regul

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Rapeseed (Brassica napus L.) is a major oilseed crop with high boron (B) requirements. In hydroponic cultivation, B uptake is highly responsive to the media conditions (e.g., pH). Different N sources, such as NH4+ and NO3−, directly affect the media pH. To date, the underlying mechanisms, the role of the buffering at constant N-sources by separating the effects of pH and/or sources of N-form, for the uptake of B remain indistinct under hydroponic cultivation, particularly in rapeseed. Two identical experiments were conducted using (NH4)2SO4 and Ca(NO3)2 as sole N-sources each set were subjected to four treatments viz. non-buffered, buffered pH 5.0, 6.0, and 7.0 at low (1 µM) and high (100 µM) B for 5 days. The novelty of this study demonstrates that NH4+-fed plants prefer buffered pH (6 and 7) for promoting higher plant growth, which was in line with the B concentration, while NO3−-fed plants were not influenced. These aforesaid findings were further supported by the upregulation of BnaNIP5;1 and BnaBOR1;2 in the root and higher expression of BnaNIP5;1 in shoots with buffered pH in NH4+-fed plants. According to our findings, in NH4+-based buffering, B transport channel protein BnaNIP5;1 (through diffusion) facilitated B uptake and distribution from root to shoot while BnaBOR1;2 mediated active uptake of B was in root under low supply of B. In a nutshell, pH buffering in hydroponics is very important in NH4+-based but not in NO3−-based nutrition for the uptake of boron and growth of the rapeseed plants.

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Exposure to high nitrogen triggered a genotype‐dependent modulation of cell and root hydraulics, which can involve aquaporin regulation

Cited by 6 publications

References 71 publications

Short-term transcriptomic analysis at organ scale reveals candidate genes involved in low N responses in NUE-contrasting tomato genotypes

Short-term transcriptomic analysis at organ scale reveals candidate genes involved in low N responses in NUE-contrasting tomato genotypes

Plant water uptake modelling: added value of cross‐disciplinary approaches

pH Stabilized NH4+-Fed Nutrition Promotes Higher B Uptake and Plant Growth in Rapeseed (Brassica napus L.) by the Upregulation of B Transporters

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