Exposure top,p′-DDE Induces Morphological Changes and Activation of the PKCα-p38-C/EBPβPathway in Human Promyelocytic HL-60 Cells

Torres-Aviles, N.A.; Albores-García, Damaris; Luna, Ana L.; Moreno-Galván, Mónica; Salgado‐Bustamante, Mariana; Portales-Pérez, Diana Patricia; Calderón-Aranda, Emma S.

doi:10.1155/2016/1375606

Cited by 9 publications

(6 citation statements)

References 58 publications

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“…As reported in most populations worldwide [ 24 , 25 , 26 ], our study found that p,p′-DDE was the most abundant compound among the target OCPs. It is the main metabolite of DDT [ 27 ], and the half-life is estimated to be as long as 8.6 years [ 28 ]. Moreover, p,p′-DDE is highly lipophilic, persists for prolonged periods in the environment, and accumulates in the food chain and the tissues of living organisms.…”

Section: Discussionmentioning

confidence: 99%

Temporal Trends of Exposure to Organochlorine Pesticides in the United States: A Population Study from 2005 to 2016

Wang

et al. 2022

IJERPH

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The current study aimed to investigate temporal trends of serum organochlorine pesticide (OCP) concentrations in the general United States population, approximately 30 years after the prohibition of OCP usage, by using National Health and Nutrition Examination Survey data. The least square geometric means and percent change in OCP concentrations were calculated by a survey weighted multiple linear regression model. Over 2005–2016, OCP concentrations showed significant downward temporal trends. Females had substantially higher concentrations of β-Hexachlorocyclohexane (β-HCH), p,p′-DDE and p,p′-DDT, but lower concentrations of Hexachlorobenzene (HCB) and trans-nonachlor. In addition, females had a more rapid decrease in p,p′-DDT levels over time than males. The overall OCP concentrations increased with age, and the two oldest age groups (aged 40–59 and 60+ years) had substantially lower rates of decrease than the younger age groups (aged 12–39 years). Concentrations and declines in OCPs (except for trans-nonachlor) were higher in Mexican Americans than both non-Hispanic Whites and non-Hispanic Blacks. There is a particular need for the ongoing monitoring of these banned chemicals, and measures should be taken to mitigate the exposure of vulnerable populations, including adults aged over 60, Mexican Americans, females for β-HCH, p,p′-DDE and p,p′-DDT, and males for HCB and trans-nonachlor.

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Section: Discussionmentioning

confidence: 99%

Temporal Trends of Exposure to Organochlorine Pesticides in the United States: A Population Study from 2005 to 2016

Wang

et al. 2022

IJERPH

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“…Adverse effects of DDE, including cellular stress generation [17,72], apoptosis [73], and mitochondrial impairment [74], have been shown in both in vitro and in vivo studies.…”

Section: Discussionmentioning

confidence: 99%

“…Adverse effects of DDE, including cellular stress generation [17,72], apoptosis [73], and mitochondrial impairment [74], have been shown in both in vitro and in vivo studies. Present work aimed to further analyze the impact of DDE on cell viability and mitochondrial parameters in an in vitro hepatic cell model (HepG2 cells) in a dose-dependent approach.…”

Section: Discussionmentioning

confidence: 99%

Dose-Dependent Response to the Environmental Pollutant Dichlorodipheniletylhene (DDE) in HepG2 Cells: Focus on Cell Viability and Mitochondrial Fusion/Fission Proteins

Burgos-Aceves

Migliaccio

Lepretti

et al. 2021

Toxics

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Dichlorodiphenyldichloroethylene (DDE), the primary persistent metabolite of dichlorodiphenyltrichloroethane (DDT), has toxic effects on cells, but its dose-dependent impact on mitochondrial proteins involved in mitochondrial fusion and fission processes associated with cell viability impairment has not yet been analysed. Mitochondrial fusion and fission processes are critical to maintaining the mitochondrial network and allowing the cell to respond to external stressors such as environmental pollutants. Fusion processes are associated with optimizing mitochondrial function, whereas fission processes are associated with removing damaged mitochondria. We assessed the effects of different DDE doses, ranging between 0.5 and 100 µM, on cell viability and mitochondrial fusion/fission proteins in an in vitro hepatic cell model (human hepatocarcinomatous cells, HepG2); the DDE induced a decrease in cell viability in a dose-dependent manner, and its effect was enhanced in conditions of coincubation with dietary fatty acids. Fusion protein markers exhibited an inverted U-shape dose-response curve, showing the highest content in the 2.5–25 μM DDE dose range. The fission protein marker was found to increase significantly, leading to an increased fission/fusion ratio with high DDE doses. The low DDE doses elicited cell adaption by stimulating mitochondrial dynamics machinery, whereas high DDE doses induced cell viability loss associated with mitochondrial dynamics to shift toward fission. Present results are helpful to clarify the mechanisms underlying the cell fate towards survival or death in response to increasing doses of environmental pollutants.

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“…However, how LSP1 deficiency is related to CEBP upregulation has not been determined. Previous studies have reported that LSP1 represses the activation of PKCβ in B cells 11 and that PKC activation induces CEBPβ expression in promyelocytic cells 23 , which raises the possibility of a LSP1-PKCβ-CEBPβ axis in LRBs. As expected, the level of phosphorylated PKCβ (pPKCβ) was higher in Lsp1 -/- B cells than in WT B cells ( Fig.…”

Section: Resultsmentioning

confidence: 95%

B lymphocytes acquire myeloid and autoimmune phenotypes via the downregulation of lymphocyte-specific protein-1

Lee,

Hong,

You

et al. 2024

Preprint

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Actin-binding proteins (ABPs) have been established as important mediators of immune homeostasis, but their effects on lymphocytes are poorly understood. Here, we demonstrated that LSP1, an ABP, is a master regulator for innate immune responses in B lymphocytes.Lsp1deficiency in B cells upregulated the expression of myeloid genes, including CD11b, CD11c, and myeloperoxidase, and bestowed myeloid morphology. Strikingly,Lsp1-deficient B cells exhibited dual functions, namely, strong phagocytic activity and high antibody (Ab) production, like ‘chimera’. The PKCβ-CEBPβ pathway was found to be required for such functional chimerism. Moreover,Lsp1deficiency induced the myeloid B cell phenotype and autoantibody production in B cells and consequently accelerated the progression of experimental lupus in mice. These changes were abrogated by retinoic acid, which upregulated LSP1 expression. In lupus patients, LSP1 expression in B cells was downregulated and inversely correlated with myeloperoxidase (MPO) expression. Overall, this study reveals a new role of the ABP LSP1 in B lymphocytes and emphasizes its critical involvement in promoting autoimmune responses, particularly by generating functionally chimeric B cells.

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Exposure top,p′-DDE Induces Morphological Changes and Activation of the PKCα-p38-C/EBPβPathway in Human Promyelocytic HL-60 Cells

Cited by 9 publications

References 58 publications

Temporal Trends of Exposure to Organochlorine Pesticides in the United States: A Population Study from 2005 to 2016

Temporal Trends of Exposure to Organochlorine Pesticides in the United States: A Population Study from 2005 to 2016

Dose-Dependent Response to the Environmental Pollutant Dichlorodipheniletylhene (DDE) in HepG2 Cells: Focus on Cell Viability and Mitochondrial Fusion/Fission Proteins

B lymphocytes acquire myeloid and autoimmune phenotypes via the downregulation of lymphocyte-specific protein-1

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