Exposure to Indoor Background Radiation and Urinary Concentrations of 8-Hydroxydeoxyguanosine, a Marker of Oxidative DNA Damage

Sperati, Alessandra; Abeni, Damiano; Tagesson, Christer; Forastiére, Francesco; Miceli, Maria; Axelson, Olav

doi:10.2307/3434511

Cited by 4 publications

(5 citation statements)

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“…[1][2][3][4][5][6] However, in a previous study, 7) only a weak correlation was observed between 8OHdG and the frequency of mutation when N,N′-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic-diimide (NP-III) was used as a source of hydroxyl radical (OH • ). NP-III is a photo-Fenton reagent known to generate OH…”

mentioning

confidence: 99%

Lack of Direct Involvement of 8‐Hydroxy‐2′‐deoxyguanosine in Hypoxanthine‐guanine Phosphoribosyltransferase Mutagenesis in V79 Cells Treated with N,N'‐Bis(2‐hydroxyperoxy‐2‐methoxyethyl)‐l,4,5,8‐naphthalenetetracarboxylic‐diimide (NP‐III) or Riboflavin

Nakajima

Takeuchi

Oginö

et al. 2002

Japanese Journal of Cancer Research

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Key words: 8-Hydroxy-2′-deoxyguanosine -hprt -Mutation -Deletion 8-Hydroxy-2′-deoxyguanosine (8OHdG), a typical oxidative marker compound in DNA, is widely used as an indicator of carcinogenesis.1-6) However, in a previous study, 7) only a weak correlation was observed between 8OHdG and the frequency of mutation when N,N′-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic-diimide (NP-III) was used as a source of hydroxyl radical (OH • ). NP-III is a photo-Fenton reagent known to generate OH• upon UVA irradiation. 8, 9) It was suggested that the damaged guanine would not directly contribute to errors in DNA that cause mutation and that the evaluation of 8OHdG would not necessarily reflect mutagenicity in a cellular system. In addition, it was possible that the mutation in our system was NP-III-dependent and would occur regardless of the generation of OH• or formation of 8OHdG. In this study, we investigated the relationship between 8OHdG and mutagenicity in V79 cells by sequence analysis of hypoxanthine-guanine phosphoribosyltransferase (hprt) gene mutants obtained through treatment with NP-III. Further, we also analyzed the effect of riboflavin (Rf), a photosensitizer known to induce the formation of 8OHdG. 10-12) MATERIALS AND METHODS Cells and reagentsChinese hamster lung fibroblasts, V79 (V79 379A, IFO#50082), were grown in Eagle's minimum essential medium supplemented with 10% heatinactivated fetal calf serum, 100 U/ml penicillin and 100 µg/ml streptomycin. NP-III was kindly provided by Dr. Seiichi Matsugo at Yamanashi University. Rf was purchased from Sigma Chemical (St. Louis, MO). Cell treatments and 8OHdG determination The cells were treated as described in a previous study. 7) Briefly, V79 cells were plated at 5×10 5 /60-mm dish and incubated for 24 h at 37°C in 5% CO 2 . The cells were washed and incubated in Dulbecco's phosphate-buffered saline for 15 min with either 20 µM NP-III or 20 µg/ml Rf under UV irradiation at 366 nm. The dose of UVA in this condition was ~10 kJ/m 2 . 7) After the treatment, the cells were washed and some were stored as a cell pellet at −80°C until the determination of 8OHdG. The determination was made under anaerobic conditions as described 13) to minimize experimental artifacts and by HPLC with an electrochemical detection system. Determination of mutagenicity and sequence analysisThe mutagenicity of the cells was evaluated by hypoxantine-guanine phosphoribosyltransferase (HPRT) mutation

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Lack of Direct Involvement of 8‐Hydroxy‐2′‐deoxyguanosine in Hypoxanthine‐guanine Phosphoribosyltransferase Mutagenesis in V79 Cells Treated with N,N'‐Bis(2‐hydroxyperoxy‐2‐methoxyethyl)‐l,4,5,8‐naphthalenetetracarboxylic‐diimide (NP‐III) or Riboflavin

Nakajima

Takeuchi

Oginö

et al. 2002

Japanese Journal of Cancer Research

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“…3 In practice, it has been reported that environmental pollutants, which produce DNA oxidative stress, such as benzene and azo dyes, induce changes in the 8-OHdG concentrations in human urine. 4,5 The development of a selective and sensitive analytical method of 8-OHdG in human urine is necessary for environmental, toxicological and clinical areas in order to study adverse oxidative reactions in human cells.…”

mentioning

confidence: 99%

Determination of 8-Hydroxy-2′-deoxyguanosine in Urine by Liquid Chromatography–Electrospray Ionization–Mass Spectrometry

Moriwaki

2000

ANAL. SCI.

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“…Η έκκριση της στα ούρα παρέχει µια εκτίµηση της συνολικής οξειδωτικής βλάβης στο DNA (Sperati 1999) και είναι ο καλύτερος δείκτης στα ούρα που υπάρχει µέχρι στιγµής (Ravanat 1999). Η συγκέντρωση της στα ούρα είναι 5-26 ngr/ml (Shigenaga 1991).…”

Section: -υδρόξυ-δεοξυγουανοσίνη (8-ohdg)unclassified

“…Η έκκριση της στα ούρα παρέχει µια εκτίµηση της συνολικής οξειδωτικής βλάβης στο DNA (Sperati 1999 1. Παθολογικά εργαστηριακά ευρήµατα και ειδικά αυτών που σχετίζονται µε τα προς µελέτη νοσήµατα του ήπατος σε προηγούµενους εργαστηριακούς ελέγχους.…”

Section: εισαγωγηunclassified

Δείκτες Οξειδωτικού Στρες Λιπιδίων, DNA Και Πρωτεϊνών Σε Χρόνιες Παθήσεις Του Ήπατος

Kaffe¹,

Καφφέ²

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Exposure to Indoor Background Radiation and Urinary Concentrations of 8-Hydroxydeoxyguanosine, a Marker of Oxidative DNA Damage

Cited by 4 publications

References 14 publications

Lack of Direct Involvement of 8‐Hydroxy‐2′‐deoxyguanosine in Hypoxanthine‐guanine Phosphoribosyltransferase Mutagenesis in V79 Cells Treated with N,N'‐Bis(2‐hydroxyperoxy‐2‐methoxyethyl)‐l,4,5,8‐naphthalenetetracarboxylic‐diimide (NP‐III) or Riboflavin

Lack of Direct Involvement of 8‐Hydroxy‐2′‐deoxyguanosine in Hypoxanthine‐guanine Phosphoribosyltransferase Mutagenesis in V79 Cells Treated with N,N'‐Bis(2‐hydroxyperoxy‐2‐methoxyethyl)‐l,4,5,8‐naphthalenetetracarboxylic‐diimide (NP‐III) or Riboflavin

Determination of 8-Hydroxy-2′-deoxyguanosine in Urine by Liquid Chromatography–Electrospray Ionization–Mass Spectrometry

Δείκτες Οξειδωτικού Στρες Λιπιδίων, DNA Και Πρωτεϊνών Σε Χρόνιες Παθήσεις Του Ήπατος

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