Exposure to lead in water and cysteine non-oxidative metabolism in Pelophylax ridibundus tissues

Kaczor, Marta; Sura, Piotr; Bronowicka-Adamska, Patrycja; Wróbel, Maria

doi:10.1016/j.aquatox.2012.03.014

Cited by 12 publications

(33 citation statements)

References 38 publications

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“…Accumulation in the testes is higher than in the brain, however, the content of all the investigated ions in these tissues is much lower in comparison to their content in the kidney and/or liver. The results are in accordance with the earlier data obtained using the X-ray fluorescence spectroscopy (XRF) and Energy Dispersion Spectrum (EDS) methods [4,5].…”

Section: Discussionsupporting

confidence: 91%

“…To perform the study, three species of anuran amphibians were selected: Pelophylax ridibundus, Xenopus laevis and Xenopus tropicalis. Two of them: Pelophylax ridibundus (Rana ridibunda-the older name) and Xenopus laevis due to our earlier investigations [4,5,23,24] and the third one-Xenopus tropicalis-due to the fact that in the NCBI database its genome appeared first. The consent to acquire marsh frogs Pelophylax ridibundus was issued by the General Director of Environment Protection (DOPozgiz-4200 / II-77/3703/10 / JRO).…”

Section: Animalsmentioning

confidence: 99%

“…In our previous studies [4,5,23,24] we postulated that sulfurtransferases (CTH, MPST and TST) can exhibit antioxidative properties. In general, the obtained results remained in line with those presented previously; however, for the first time, in the present paper, we have shown changes of the sulfurtransferase activity results in comparison to the level of their gene expression.…”

Section: Introductionmentioning

confidence: 99%

“…Redox-active metals such as Cu catalyze Haber-Weiss/Fenton reaction generating oxidizing radicals (Figure 1). The cellular targets for heavy metal toxicity especially include such organs as the kidney, liver, heart and testicles, as well as the immune and nervous systems [2][3][4][5]. Figure 1.…”

Section: Introductionmentioning

confidence: 99%

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Multidirectional Changes in Parameters Related to Sulfur Metabolism in Frog Tissues Exposed to Heavy Metal-Related Stress

2020

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The investigations showed changes of the cystathionine γ-lyase (CTH), 3-mercaptopyruvate sulfurtransferase (MPST) and rhodanese (TST) activity and gene expression in the brain, heart, liver, kidney, skeletal muscles and testes in frogs Pelophylax ridibundus, Xenopus laevis and Xenopus tropicalis in response to Pb 2+ , Hg 2+ and Cd 2+ stress. The results were analyzed jointly with changes in the expression of selected antioxidant enzymes (cytoplasmic and mitochondrial superoxide dismutase, glutathione peroxidase, catalase and thioredoxin reducatase) and with the level of malondialdehyde (a product of lipid peroxidation). The obtained results allowed for confirming the role of sulfurtransferases in the antioxidant protection of tissues exposed to heavy metal ions. Our results revealed different transcriptional responses of the investigated tissues to each of the examined heavy metals. The CTH, MPST and TST genes might be regarded as heavy metal stress-responsive. The CTH gene expression up-regulation was confirmed in the liver (Pb 2+ , Hg 2+ , Cd 2+ ) and skeletal muscle (Hg 2+ ), MPST in the brain (Pb 2+ , Hg 2+ ), kidney (Pb 2+ , Cd 2+ ), skeletal muscle (Pb 2+ , Hg 2+ ,Cd 2+ ) and TST in the brain (Pb 2+ ) and kidney (Pb 2+ , Hg 2+ , Cd 2+ ). Lead, mercury and cadmium toxicity was demonstrated to affect the glutathione (GSH) and cysteine levels, the concentration ratio of reduced to oxidized glutathione ([GSH]/[GSSG]) and the level of sulfane sulfur-containing compounds, which in case of enhanced reactive oxygen species generation can reveal their antioxidative properties. The present report is the first to widely describe the role of the sulfane sulfur/H 2 S generating enzymes and the cysteine/glutathione system in Pb 2+ , Hg 2+ and Cd 2+ stress in various frog tissues, and to explore the mechanisms mediating heavy metal-related stress.

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Section: Discussionsupporting

confidence: 91%

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multidirectional Changes in Parameters Related to Sulfur Metabolism in Frog Tissues Exposed to Heavy Metal-Related Stress

2020

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“…In vivo experiments showed that CSE-knockout mice were sensitive to Cd-induced hepatotoxicity [44]. Adaptive changes in the activity and expression of CGL, MST, and TST in various frog tissues in response to exposure to lead, mercury, and cadmium confirmed the protective function of these enzymatic proteins against electrophilic stress [45,46].…”

Section: Physiological Functions Of H 2 S and Polysulfidesmentioning

confidence: 85%

H2S, Polysulfides, and Enzymes: Physiological and Pathological Aspects

Nagahara

Wróbel

2020

Biomolecules

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We have been studying the general aspects of the functions of H 2 S and polysulfides, and the enzymes involved in their biosynthesis, for more than 20 years. Our aim has been to elucidate novel physiological and pathological functions of H 2 S and polysulfides, and unravel the regulation of the enzymes involved in their biosynthesis, including cystathionine β-synthase (EC 4.2.1.22), cystathionine γ-lyase (EC 4.4.1.1), thiosulfate sulfurtransferase (rhodanese, EC 2.8.1.1), and 3-mercaptopyruvate sulfurtransferase (EC 2.8.1.2). Physiological and pathological functions, alternative biosynthetic processes, and additional functions of H 2 S and polysulfides have been reported. Further, the structure and reaction mechanisms of related enzymes have also been reported. We expect this issue to advance scientific knowledge regarding the detailed functions of H 2 S and polysulfides as well as the general properties and regulation of the enzymes involved in their metabolism. We would like to cover four topics: the physiological and pathological functions of H 2 S and polysulfides, the mechanisms of the biosynthesis of H 2 S and polysulfides, the properties of the biosynthetic enzymes, and the regulation of enzymatic activity. The knockout mouse technique is a useful tool to determine new physiological functions, especially those of H 2 S and polysulfides. In the future, we shall take a closer look at symptoms in the human congenital deficiency of each enzyme. Further studies on the regulation of enzymatic activity by in vivo substances may be the key to finding new functions of H 2 S and polysulfides.

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Histopathological examination and transcriptome analyses to assess the acute toxic effects of arsenite exposure on rare minnows (Gobiocypris rarus)

Shi

Wang

et al. 2020

Ecotoxicology

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Exposure to lead in water and cysteine non-oxidative metabolism in Pelophylax ridibundus tissues

Cited by 12 publications

References 38 publications

Multidirectional Changes in Parameters Related to Sulfur Metabolism in Frog Tissues Exposed to Heavy Metal-Related Stress

Multidirectional Changes in Parameters Related to Sulfur Metabolism in Frog Tissues Exposed to Heavy Metal-Related Stress

H2S, Polysulfides, and Enzymes: Physiological and Pathological Aspects

Histopathological examination and transcriptome analyses to assess the acute toxic effects of arsenite exposure on rare minnows (Gobiocypris rarus)

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