Exposure to low-dose arsenic caused teratogenicity and upregulation of proinflammatory cytokines in zebrafish embryos

Piyushbhai, Modi Kiran; Binesh, Ambika; Shanmugam, S. A.; Venkatachalam, Karthikeyan

doi:10.1007/s12011-022-03418-w

Cited by 7 publications

(1 citation statement)

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“…Fish maintenance and embryo collection were performed as described previously. [32] 2.2 | Exposure of ZFE to mercury chloride A 1 mM stock solution of mercuric (II) chloride (HgCl 2 ) was prepared by dissolving the crystalline powder in distilled water to prepare solutions of varying concentrations (100, 200, 300, 400, 500, and 600 nM) in an embryo medium. To mitigate spontaneous mortality rates ranging from 5% to 25%, we exposed ZFE to varying concentrations of HgCl 2 in Petri dishes at 24…”

Section: Fish Husbandry and Collection Of Embryosmentioning

confidence: 99%

Effects of mercury chloride on antioxidant and inflammatory cytokines in zebrafish embryos

Bhai,

Binesh,

Shanmugam

et al. 2023

J Biochem & Molecular Tox

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In this study, a zebrafish embryo toxicity model was employed, utilizing 24 h postfertilization (hpf) zebrafish embryos. These embryos were treated with varying concentrations of mercuric chloride for 96 h under static conditions. We assessed multiple parameters that reflected developmental abnormalities, behavioral alterations, morphological anomalies, antioxidant enzyme activities, including those of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione S‐transferase (GST), immune messenger RNA transcription levels of key factors such as tumor necrosis factor α (TNF‐α), interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and cyclooxygenase 2 (COX‐2), as well as protein expression of TNF‐α. The results revealed that embryos exposed to higher concentrations of mercury exhibited reduced hatchability and increased rates of morphological abnormalities and mortality at 48, 72, and 96 hpf. In addition, a concentration‐dependent increase in developmental abnormalities, including cardiac edema, reduced body length, yolk sac edema, scoliosis, and bent tails, was observed. Larval behaviors, such as touch‐induced escape responses, startle reactions, and turning actions, were found to be diminished in a concentration‐dependent manner. Additionally, the activities of various antioxidative enzymes, such as SOD, CAT, and GST, exhibited an increase at higher mercury concentrations, with the exception of GPX activity, which decreased significantly in a dose‐dependent manner (p < 0.05). Pro‐inflammatory cytokine transcription levels, specifically TNF‐α, IL‐1β, IL‐6, and COX‐2, were significantly upregulated in a dose‐dependent manner in the mercuric (II) chloride (HgCl2) treatment group compared with the control group. TNF‐α protein expression was notably elevated in the larvae group treated with 300 and 400 nM HgCl2.

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Section: Fish Husbandry and Collection Of Embryosmentioning

confidence: 99%

Effects of mercury chloride on antioxidant and inflammatory cytokines in zebrafish embryos

Bhai,

Binesh,

Shanmugam

et al. 2023

J Biochem & Molecular Tox

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Poison in the water: Arsenic's silent assault on fish health

Chandel,

Sharma,

Thakur

et al. 2024

J of Applied Toxicology

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Arsenic occurs across the world in freshwater and marine environments, menacing the survival of aquatic organisms. Organic and inorganic forms of this substance can be found, in which the inorganic form is more hazardous than the organic form. Most aquatic bodies contain inorganic arsenic species, but organic species are believed to be the dominant form of arsenic in the majority of fish. Natural and anthropogenic both are the sources of water contamination with arsenic. Its bioaccumulation and transfer from one trophic level to another in the aquatic food chain make arsenic a vital environmental issue. Continuous exposure to low concentrations of arsenic in aquatic organisms including fish leads to its bioaccumulation, which may affect organisms of higher trophic levels including large fishes or humans. Humans can be exposed to arsenic through the consumption of fish contaminated with arsenic. Hence, the present review facilitates our understanding about sources of arsenic, its bioaccumulation, food chain transfer, and its effect on the fish health. Also, “Poison in the Water: Arsenic's Silent Assault on Fish Health” serves as a wake‐up call to recognize the pressing need to address arsenic contamination in water bodies. By understanding its devastating impact on fish health, we can strive to implement sustainable practices and policies that safeguard our precious aquatic environments and ensure the well‐being of both wildlife and human communities that depend on them.

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Copper in Human Health and Disease: A Comprehensive Review

Binesh,

Venkatachalam

2024

J Biochem & Molecular Tox

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This comprehensive review discusses the crucial role of copper in human health and disease as an essential trace mineral. It emphasizes the significance of copper while addressing potential risks from imbalances in copper levels, be it excessive or inadequate. The review outlines various challenges in copper research, including toxicity concerns, data limitations, metabolic complexities, genetic influences, nutrient interactions, and resource constraints. Despite these challenges, the review identifies specific research areas needing exploration, such as copper homeostasis regulation, transport mechanisms, gut microbiome interactions, immune function, neurodegenerative diseases, cardiovascular health, cancer, fertility, and reproductive health. The purpose of this review is to explore the important role of copper in human health and disease, which highlights the delicate balance required to avoid deficiency or toxicity. For the researchers and scientists, it provides the gaps in the research, so it aims to provide insights that could advance diagnostic and therapeutic strategies across various medical disciplines.

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Exposure to low-dose arsenic caused teratogenicity and upregulation of proinflammatory cytokines in zebrafish embryos

Cited by 7 publications

References 50 publications

Effects of mercury chloride on antioxidant and inflammatory cytokines in zebrafish embryos

Effects of mercury chloride on antioxidant and inflammatory cytokines in zebrafish embryos

Poison in the water: Arsenic's silent assault on fish health

Copper in Human Health and Disease: A Comprehensive Review

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