Exposure to Supplemental Oxygen and Its Effects on Oxidative Stress and Antioxidant Enzyme Activity in Term Newborn Lambs

Kumar, Vasantha H.S.; Patel, A.; Swartz, Daniel D.; Wang, Huamei; Wynn, Karen; Nielsen, Lori; Ryan, Rita M.

doi:10.1203/pdr.0b013e3181bf587f

Cited by 17 publications

(15 citation statements)

References 30 publications

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“…Resuscitation of newborn piglets with 100% oxygen after acute hypoxia increased MMP-2 activity in the brain compared to 21% resuscitation, but animals exposed to 30 min of 100% oxygen without previous hypoxia did not demonstrate these changes 32 . Similar to our findings on SOD, there were no differences found in anti-oxidant enzymes (SOD, catalase, or glutathione peroxidase) between term lambs ventilated with oxygen or air 33 .…”

Section: Discussionsupporting

confidence: 90%

Moderate tidal volumes and oxygen exposure during initiation of ventilation in preterm fetal sheep

et al. 2012

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Background Preterm infants often receive mechanical ventilation and oxygen at birth. Exposure to large tidal volumes (VT) at birth causes lung inflammation and oxygen may amplify the injury. We hypothesized that normal VT ventilation at birth causes lung injury that is exacerbated by 95% oxygen. Methods The head and chest of anesthetized preterm fetal sheep (129±1d gestation) were surgically exteriorized while maintaining the placental circulation. Fetuses were randomized to four groups with either: 1) VT ventilation to 6 mL/kg or 2) CPAP of 5 cm H2O, and either: a) 95%O2/5%CO2 or b) 95%N2/5%CO2. Age-matched fetuses were controls. After a 15-minute intervention, the fetal lamb was returned to the uterus for 1 h 45 min. Results In ventilated lambs, VT was 6.2±0.4 mL/kg at 15 min. Ventilation increased pro-inflammatory cytokines compared to control and CPAP only lambs, with recruitment of primarily monocytes to bronchioalveolar lavage fluid. Early response protein 1 was activated around the bronchioles in VT ventilated animals. The 15-min oxygen exposure did not change inflammatory mediators or other markers of lung and oxidative stress. Conclusions A VT of 6–7 mL/kg at birth increased early markers of injury and lung inflammation. Brief exposure to 95% oxygen did not alter lung inflammation.

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Section: Discussionsupporting

confidence: 90%

Moderate tidal volumes and oxygen exposure during initiation of ventilation in preterm fetal sheep

et al. 2012

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“…The management of asphyxiated infants usually starts with resuscitation and involves mechanical ventilation. Reoxygenation of the tissues is a primary goal of resuscitation efforts; however, reoxygenation and reperfusion provide oxygen for the synthesis of reactive oxygen species (ROS) that can result in oxidative stress and subsequent reoxygenation/ reperfusion injury (5)(6)(7)(8)(9). Resuscitation/ventilation with pure oxygen has been shown to be detrimental in neonatal large animal models (10 -12) and in clinical trials as well (13,14).…”

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confidence: 99%

Hydrogen is neuroprotective and preserves cerebrovascular reactivity in asphyxiated newborn pigs

et al. 2010

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“…Catalase activity was found to be decreased with the increase in noise exposure duration. decreased catalase activity indicates high oxidative damage [9]. These findings indicate that antioxidant enzyme activity can neutralize the drastic effects of noise for a certain level.…”

Section: Discussionmentioning

confidence: 72%

Effects of noise exposure on catalase activity of growing lymphocytes

Nawaz

Hasnain

2012

Bosn J of Basic Med Sci

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Oxidative stress due to noise was estimated at cell level using model of growing lymphocytes. Lymphocytes were isolated and cultured using conventional methodology. Cell culture of each group was exposed to sound of frequency 1 KHz during incubation. Three groups were defined on the basis of exposure of sound with specific range of intensity and duration of exposure. Group A and Group B were exposed to sound with intensity 110 dBA for four hours per day and for eight hours per day respectively. Control group was exposed to sound less than 85 dBA. Viable cell count was performed using trypan blue. Catalase activity of each group was estimated using ELISA kit. Viable cell count of Group A and Group B was almost same but significantly less than that of control group. Catalase activity of lymphocytes in Group B was significantly low as compared to Group A and controls (p=0.003, p< 0.05). There was no significant difference between catalase activity of Group A and control group. Exposure of sound with frequency 1 KHz and intensity 110 dBA for 4 hours and eight hours per day may induce oxidative stress in growing lymphocytes causing the difference in viable cell count. However the catalase activity depends on duration of exposure. In case of noise exposure of 8 hours per day, it declines significantly as compared to noise exposure of 4 hours per day.

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Exposure to Supplemental Oxygen and Its Effects on Oxidative Stress and Antioxidant Enzyme Activity in Term Newborn Lambs

Cited by 17 publications

References 30 publications

Moderate tidal volumes and oxygen exposure during initiation of ventilation in preterm fetal sheep

Moderate tidal volumes and oxygen exposure during initiation of ventilation in preterm fetal sheep

Hydrogen is neuroprotective and preserves cerebrovascular reactivity in asphyxiated newborn pigs

Effects of noise exposure on catalase activity of growing lymphocytes

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