Exposure to Swine Housing Dust Modulates Macrophage Morphology and Function

Pender, Ruth J.; Minor, Radiah C.; Hurley, Steven; Conklin, Dawn; Waterman, Jenora T.

doi:10.3844/ajisp.2014.35.45

Cited by 5 publications

(6 citation statements)

References 25 publications

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“…This data is consistent with Pender et al, which demonstrates that hog barn dust induces ROS in vitro [24]. We demonstrate that lactate dehydrogenase (LDH) levels increase in a dose-dependent manner which complements the data that shows that hog barn dust induces 8-isoprostane, a biomarker of lipid peroxidation.…”

Section: Discussionsupporting

confidence: 92%

“…Hog barn dust exposure induces a myriad of complications on the respiratory epithelium, and facility workers are at risk for development of respiratory symptoms and disease [1]. In Pender et al [24], results show that hog barn dust (5 and 10%) induces ROS in a dose-dependent manner in THP-1 cells. Wyatt et al reported that hog barn dust increased nitric oxide levels in a time-dependent manner in bovine ciliated cells [27].…”

Section: Discussionmentioning

confidence: 99%

“…Settled dust was collected from raised surfaces within the swine confinement facility at North Carolina A&T State University, and DE was prepared by dissolving dust (1 g) in HBSS, centrifugation, and 0.2 μ m filtration as was previously described [24].…”

Section: Methodsmentioning

confidence: 99%

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Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro

Gerald

McClendon

Ranabhat

et al. 2019

Mediators of Inflammation

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Exposure to hog barn organic dust contributes to occupational lung diseases, which are mediated by inflammatory and oxidative stress pathways. Isoprostanes—a family of eicosanoids produced by oxidation of phospholipids by oxygen radicals—are biomarkers of pulmonary oxidative stress. Importantly, 8-isoprostane has been implicated as a key biomarker and mediator of oxidative stress because it is a potent pulmonary vasoconstrictor. Antioxidants found in fruits and vegetables hold promise for preventing or reducing effects of oxidative stress-related diseases including chronic bronchitis and chronic obstructive pulmonary disease (COPD). Here, we investigated 8-isoP and oxidant production by organic dust-exposed airway epithelial cells and the inhibitory effects of an extract from calyces of the sorrel plant, Hibiscus sabdariffa, on oxidant-producing pathways. Confluent cultures of normal human tracheobronchial epithelial cells were pretreated or not with 1% sorrel extract prior to 5% dust extract (DE) exposure. Following DE treatments, live cells, cell-free supernatants, or cell extracts were evaluated for the presence of 8-isoprostane, superoxide, hydrogen peroxide, nitric oxide, hydroxyl radical, peroxynitrite, and catalase activity to evaluate sorrel’s inhibitory effect on oxidative stress. The well-known radical scavenging antioxidant, N-acetyl cysteine (NAC), was used for comparisons with sorrel. DE exposure augmented the production of all radicals measured including 8-isoprostane (p value < 0.001), which could be inhibited by NAC or sorrel. Among reactive oxygen and nitrogen species generated in response to DE exposure, sorrel had no effect on H2O2 production and NAC had no significant effect on NO⋅ production. The observations reported here suggest a possible role for sorrel in preventing 8-isoprostane and oxidant-mediated stress responses in bronchial epithelial cells exposed to hog barn dust. These findings suggest a potential role for oxidative stress pathways in mediating occupational lung diseases and antioxidants within sorrel and NAC in reducing dust-mediated oxidative stress within the airways of exposed workers.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro

Gerald

McClendon

Ranabhat

et al. 2019

Mediators of Inflammation

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“…There are studies demonstrating that hog barn dust induces oxidants. In Pender et al, THP-1 cells that were exposed to hog barn dust (5 and 10 %) show increased levels of ROS production (Pender et al, 2014). Wyatt et al show increased NO in bovine airway cells exposed to hog barn dust (Wyatt et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Alcohol Decreases Organic Dust‐Stimulated Airway Epithelial TNF‐Alpha Through a Nitric Oxide and Protein Kinase‐Mediated Inhibition of TACE

Gerald

Romberger

DeVasure

et al. 2016

Alcoholism Clin & Exp Res

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Background Farm workers in rural areas consume more alcohol than those who reside in urban areas. Occupational exposures such as agricultural work can pose hazards on the respiratory system. It is established that hog barn dust induces inflammation in the airway, including the release of cytokines such as tumor necrosis factor alpha (TNFα), interleukin-6 (IL-6) and interleukin-8 (IL-8). We have shown that alcohol alters airway epithelial innate defense through changes in both nitric oxide (NO) and cAMP-dependent protein kinase (PKA). Simultaneous exposure to hog barn dust and alcohol decreases inflammatory mediators, TNFα, IL-6 and IL-8, in mice. Previously, mice exposed to both alcohol and hog barn dust showed a depleted amount of lymphocytes compared to mice exposed only to hog barn dust. Weakening of the innate immune response could lead to enhanced susceptibility to disease. In addition, mice that were co-exposed to hog barn dust and alcohol also experienced increased mortality. Methods Because we recently demonstrated that PKA activation inhibits the TNFα sheddase, TNF-α converting enzyme (TACE), we hypothesized that an alcohol-mediated PKA pathway blocks TACE activity and prevents the normative inflammatory response to hog barn dust exposure. To delineate these effects, we used PKA pathway inhibitors (adenylyl cyclase, cAMP and PKA) to modulate the effects of alcohol on dust-stimulated TNFα release in the bronchial epithelial cell line, BEAS-2B. Alcohol pretreatment blocked TACE activity and TNFα release in hog barn dust-treated cells. Results Alcohol continued to block hog barn dust-mediated TNFα release in the presence of the particulate adenylyl cyclase inhibitor, SQ22,536. The soluble adenylyl cyclase (sAC) inhibitor, KH7, however, significantly increased the inflammatory response to hog barn dust. PDE4 inhibitors significantly elevated cAMP and enhanced alcohol-mediated inhibition of dust-stimulated TNFα release. In addition, the nitric oxide synthase inhibitor, L-NMMA, also reversed the alcohol-blocking effect on dust-stimulated TNFα. Conclusions These data suggest that alcohol requires a soluble cyclase-generated cAMP-PKA pathway that is dependent upon the action of NO to inhibit TACE and TNFα release. These findings support our observations that alcohol functions through a dual NO and PKA pathway in bronchial epithelial cells.

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“…Dust extracts were prepared as previously described (Pender et al, 2014). Briefly, one gram of swine facility dust was combined with 10 mL of phosphate buffered saline solution without calcium and magnesium vortexed for 1 min.…”

Section: Preparation Of Dust Extractsmentioning

confidence: 99%

Moringa Tea Blocks Acute Lung Inflammation Induced by Swine Confinement Dust Through a Mechanism Involving TNF-Î± Expression, C-Jun N-Terminal Kinase Activation and Neutrophil Regulation

McKnight¹

2014

American Journal of Immunology

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Plant based products represent a promising alternative to conventional treatments for inflammation. Moringa oleifera Lam is a tree rich in proteins, vitamins, minerals and a variety of phytochemcals with health benefits. Among the reported health benefits are antioxidant and anti-inflammatory properties. The purpose of this study was to investigate whether tea brewed from dried Moringa leaves would abrogate inflammation in a mouse model of acute lung inflammation induced by LPS or extracts prepared from dust collected from a swine confinement facility (DE). Mice were offered water or Moringa tea for seven days. Tea consumption was significantly greater than that of water consumption on days 1 and 6, but there were no significant differences in weight gain or food consumption. On day seven, mice from both groups were forced to inhale, via intranasal challenge, either Phosphate Buffered Saline (PBS), Lipopolysaccharide (LPS) [10 µg mLCompared to mice that drank water, mice that drank Moringa tea had significantly less protein (p<0.05) and cellular influx (p<0.0001) into the lung after inhalation of 10% DE. No difference in neutrophil migration into the lungs of water and M. tea groups after LPS or DE challenge was detected. But mice that drank tea had significantly (p<0.05) more neutrophils with apoptotic morphology after DE challenge. TNF-α expression 24 h after inhalation of 10% DE, was significantly higher (p<0.05) in lungs of M. tea mouse group as compared to water group. This increase in TNF-α was accompanied by higher levels of pro and anti-inflammatory cytokines. Finally, activation of c-Jun N-terminal Kinase (JNK) in lungs of M. tea+DE group 24 h post inhalation was decreased. Taken together these results suggest that Moringa oleifera leaf tea exerts antiinflammatory properties on acute lung inflammation induced by swine confinement dust through a mechanism involving neutrophil regulation and JNK activation.

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Exposure to Swine Housing Dust Modulates Macrophage Morphology and Function

Cited by 5 publications

References 25 publications

Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro

Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro

Alcohol Decreases Organic Dust‐Stimulated Airway Epithelial TNF‐Alpha Through a Nitric Oxide and Protein Kinase‐Mediated Inhibition of TACE

Moringa Tea Blocks Acute Lung Inflammation Induced by Swine Confinement Dust Through a Mechanism Involving TNF-Î± Expression, C-Jun N-Terminal Kinase Activation and Neutrophil Regulation

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