Exposure to the seminal plasma of different portions of the boar ejaculate modulates the survival of spermatozoa cryopreserved in MiniFlatPacks

Saravia, F.; Wallgren, Margareta; Johannisson, Anders; Calvete, Juan J.; Sanz, Libia; Peña, Francisco; Roca, Jordi; Rodriguez‐Martinez, Heriberto

doi:10.1016/j.theriogenology.2008.09.037

Cited by 73 publications

(93 citation statements)

References 62 publications

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“…Previous results confirmed the existence of essential differences between the P1 and the rest of the ejaculate (Rodríguez-Martínez et al, 2009;Saravia et al, 2009) related to types and amounts of ions, proteins, bicarbonate and pH indicating that the P1 spermatozoa were still bathing in a substantial amount of fluid from the cauda epididymides, in which they were emitted at ejaculation, and that this would explain the better performance of the spermatozoa contained in this SRF-portion. The findings of the current study, have clearly shown that despite the SRF-P1 having a significantly larger relative 14 contents of total protein than the P1, these portions of the SRF displayed very similar protein profiles as assessed by 2DE and mass spectrometry (tryptic peptide mass fingerprint analysis and CID-MS/MS) (Fig.2).…”

supporting

confidence: 59%

“…the sperm peak-portion of the SRF (P1), appeared suitable for the more extensive use of frozen-thawed boar semen, since this protocol reduced the time involved in the handling of the semen (8-9 h are usually required to process an ejaculate using the current technique), diminished the inter-boar variability in the cryopreservation (probably as a concerted action of by the SP-composition of the P1 and the packing in cryobiologically advantageous containers such as the MFP; Saravia et al, 2009), and allow to use the rest of the ejaculate to produce routine liquid semen doses for conventional AI (Saravia et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Cryopreservation of boar semen has advanced over the past decade, with inclusion of various additives in the semen extender (Peña et al, 2003a(Peña et al, , 2004Roca et al, 2004Roca et al, , 2005, novel packaging systems (Eriksson & Rodriguez-Martinez, 2000;Saravia et al, 2009), and simplified freezing protocols (Saravia 4 et al, 2010). The latter was possible by findings in this laboratory, which demonstrated that the spermpeak portion of the ejaculate (the first 10 mL of the SRF, P1) can equally sustain conventional or simplified cryopreservation and -when this is done-more than 50% of the time allotted for conventional freezing is saved, avoiding extension or centrifugation.…”

Section: Introductionmentioning

confidence: 99%

“…Cross-exposure of spermatozoa from either portion (P1 vs P2) to their native SP could clearly influence their cryosurvival, thus reinforcing the above concept: particular portions of the SP modulate sperm structure, intactness and, ultimately, function ). Specifically, differences in SP proteomics have been described Saravia et al, 2009) and different SP protein profiles have been found between boars of different in vivo fertility (Flowers and Turner, 2001). Whether there are significant differences in proteomics between portions of the SRF, regarding SP-proteins related to sperm viability or fertility remains to be studied.…”

Section: Introductionmentioning

confidence: 99%

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Quality of boar spermatozoa from the sperm-peak portion of the ejaculate after simplified freezing in MiniFlatpacks compared to the remaining spermatozoa of the sperm-rich fraction

Siqueira

Wallgren

Hossain³

et al. 2011

Theriogenology

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Quality of boar spermatozoa from the sperm-peak portion of the ejaculate after simplified freezing in MiniFlatpacks compared to the remaining spermatozoa of the sperm-rich fraction, 2011 , THERIOGENOLOGY, (75), 7, 1175 -1184 AbstractBoar sperm viability post-thaw differs depending on the ejaculate fraction used, with spermatozoa present in the first 10mL of the sperm-rich fraction, SRF (portion 1, P1, sperm-peak portion), showing the best cryosurvival in vitro when compared with spermatozoa from the rest of the ejaculate (second portion of the SRF plus the post-spermatic fraction), even when using simplified freezing routines. Such abilities apparently relate to the specific composition of seminal plasma in the P1 (glycoproteins, pH, and bicarbonate concentrations). However, spermatozoa from P1 have not been compared to the rest of spermatozoa in the SRF (SRF-P1, usually 30 to 40 mL of the SRF), which is routinely used for freezing.Such comparison of P1 vs SRF-P1 was hereby done, in terms of sperm kinematics (QualiSperm TM system), membrane integrity (SYBR-14/PI), acrosome integrity (FITC PNA/PI), and sperm membrane stability (Annexin-V), explored using flow cytometry. As well, total protein concentration and the proteomics of the seminal plasma (SP) of either portion of the SRF were studied using two-dimensional electrophoresis (2DE), mass fingerprinting (MALDI-TOF) and collision-induced dissociation tandem mass spectrometry (CID-MS/MS) of selected peptides. The SRF portions were weekly collected from four mature boars (4-5 replicates per boar, sperm concentration: P1-1.86±0.20, SRF-P1-1.25±0.14 ×10 9 spz/mL) and processed using a quick freezing method in MiniFlatPacks (MFP). Sperm motility post-thaw reached 50%, without differences between portions of the SRF, but with clear inter-boar variation. Neither did plasma membrane or acrosome integrity differ (ns) between fractions. These results indicate that there are no differences in cryosurvival after quick freezing of boar spermatozoa derived from either of the two portions of the SRF. While P1 and SRF-P1 clearly differed in relative total protein contents, as expected, they displayed very similar protein profiles as assessed by 2DE and mass spectrometry (tryptic peptide mass fingerprint analysis and CID-MS/MS), indicating a similar emission of epididymal protein content.3

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supporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Quality of boar spermatozoa from the sperm-peak portion of the ejaculate after simplified freezing in MiniFlatpacks compared to the remaining spermatozoa of the sperm-rich fraction

Siqueira

Wallgren

Hossain³

et al. 2011

Theriogenology

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“…Introduction: Boar spermatozoa behave differently to cryopreservation process depending on the ejaculate portion they are delivered in (Saravia et al 2009) apparently explained by quantitative differences in some SP-components (Alkmin et al 2014). This study evaluated if differences in SP-TAC among ejaculate portions might explain differences in sperm cryosurvival.…”

Section: St 11mentioning

confidence: 99%

Short Talks (ST)

2015

Reprod Domestic Animals

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Introduction: Low sperm quality is a main reason for culling of boars in AI centers. Often a series of ejaculates is examined before the decision for culling is made. In this study, the association between clinical genital results and sperm quality in a Pietrain boar population was studied and the economic value of early clinical diagnosis in boars with low sperm quality was investigated. Materials and methods: 145 adult (13-121 month) and 97 young (7-12 month) Pietrain boars housed in a German AI stud were regularly examined over a 13 month period. During semen collection all animals were clinical-andrological examined including visual evaluation, palpation and measurement of testis, epididymis and the spermatic cord. A pelvimeter, model Martin, was used for all measurements. The semen was extended and analyzed for sperm motility (0 h, 24 h, 72 h) with a CASA system (SpermVision Ò , Minit€ ub Germany). Sperm morphology was microscopically examined on 200 formol-citrat fixated cells at magnification 91000 with oil immersion. The integrity of plasma and acrosome membranes was evaluated by flow cytometry using propidium iodide (PI) and FITC-conjugated peanut agglutinin (FITC-PNA) as described by Henning et al. (2012). Results of genital organ and semen evaluation were dichotomized. The semen parameters were classified according to the semen quality standards of the Umbrella Organization of German Pig Producers (ZDS, 2005). The threshold for membrane integrity (PI neg. and FITC-PNA neg. cells) was set according to the 75% percentile. Odds-ratios (OR) were created to show the potential risk for boars with persistent abnormal genital results to produce low quality semen. The economic value of early clinical diagnosis in boars with low sperm quality was calculated in different case scenarios considering regular costs for boar housing of 6.50 € per day, 22.90 € for the clinical examination by a veterinarian. Results: Abnormal testis revealed a high risk for teratozoospermia (OR 17.0,). Seven out of nine boars with abnormal testis showed increased numbers for morphologic abnormal sperm. Soft testis texture and scrotal fluctuation were as the most common genital anomalies. 23.4% of adult boars and 7.2% of the young boar showed enlarged diameter and soft texture of the spermatic cord, diagnosed as a varicocele testis. For boars with varicocele testis significant ORs for teratozoospermia (2.4, Cl = 1.1-5.0) and membrane damage (2.0, Cl = 1.0-4.1) were found. 53.7% of boars with low sperm quality showed abnormal genital results within eight weeks prior to culling. Clinical examination of genital organs of adult boars with suspicious semen quality could accelerate the decision for culling by six weeks. In this case, the andrological examination reveals cost savings of 124.52 € per boar. 36.4% of the young boars showed dysspermia together with suspicious genital organ results. In young boars with dysspermia, the cost benefit of an early andrology result strongly depends on quarantine costs and if semen quality is already eval...

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Measurement of activity and concentration of paraoxonase 1 (PON‐1) in seminal plasma and identification of PON‐2 in the sperm of boar ejaculates

Barranco

Roca

Tvarijonaviciute

et al. 2014

Molecular Reproduction Devel

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Abbreviations: BTS, Beltsville thawing solution; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; LPO, peroxidation of membrane lipids; OS, oxidative stress; PBS, EDTA-free phosphate-buffered saline; PON, paraoxonase; ROS, reactive oxygen species; RT, room temperature; SP, seminal plasma. 3 ABSTRACTThis study revealed and characterised the presence of the antioxidant enzymes paraoxonase (PON) type 1 (PON-1, extracellular) and type 2 (PON-2, intracellular) in boar semen. To evaluate PON-1, an entire ejaculate from each of ten boars was collected and the seminal plasma (SP) harvested after double centrifugation (1,500g; 10 min). SP was analysed for concentration as well as enzymatic activity of PON-1, and for total cholesterol levels.

show abstract

Exposure to the seminal plasma of different portions of the boar ejaculate modulates the survival of spermatozoa cryopreserved in MiniFlatPacks

Cited by 73 publications

References 62 publications

Quality of boar spermatozoa from the sperm-peak portion of the ejaculate after simplified freezing in MiniFlatpacks compared to the remaining spermatozoa of the sperm-rich fraction

Quality of boar spermatozoa from the sperm-peak portion of the ejaculate after simplified freezing in MiniFlatpacks compared to the remaining spermatozoa of the sperm-rich fraction

Short Talks (ST)

Measurement of activity and concentration of paraoxonase 1 (PON‐1) in seminal plasma and identification of PON‐2 in the sperm of boar ejaculates

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