2000
DOI: 10.1177/096368970000900309
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Exposure to Tissue Culture Conditions Can Adversely Affect Myoblast Behavior in Vivo in Whole Muscle Grafts: Implications for Myoblast Transfer Therapy

Abstract: The effects of tissue culture conditions on the viability of myoblasts in whole muscles transplanted in vivo were investigated. Whole male (SJL/J) donor muscles were exposed to various tissue culture reagents and proteolytic enzymes, and allografted into female (SJL/J) host mice. Desmin immunohistochemistry was used to assess the numbers of myogenic cells (as an index of myoblast viability and the extent of regeneration) in tissue sections of whole-muscle grafts sampled on days 7 and 14. DNA quantitation with … Show more

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Cited by 62 publications
(39 citation statements)
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“…mES cells undergo chondrogenic differentiation by cytokine stimulation or different culture conditions (Kramer et al, 2000;Hegert et al, 2002;Tanaka et al, 2004) under a long-term culture system. However, the prolonged differentiation systems would delay the treatment to patients or could alter the immunogenicity of cultured autologous cells, ultimately leading to immunorejection upon transplantation (Hodgetts et al, 2000;Smythe et al, 2000;Heng et al, 2004). To enhance the chondrogenic differentiation through genetic modification, we successfully established stable cell lines constitutively expressing exogenous SOX9 in mES cells.…”
Section: Discussionmentioning
confidence: 99%
“…mES cells undergo chondrogenic differentiation by cytokine stimulation or different culture conditions (Kramer et al, 2000;Hegert et al, 2002;Tanaka et al, 2004) under a long-term culture system. However, the prolonged differentiation systems would delay the treatment to patients or could alter the immunogenicity of cultured autologous cells, ultimately leading to immunorejection upon transplantation (Hodgetts et al, 2000;Smythe et al, 2000;Heng et al, 2004). To enhance the chondrogenic differentiation through genetic modification, we successfully established stable cell lines constitutively expressing exogenous SOX9 in mES cells.…”
Section: Discussionmentioning
confidence: 99%
“…70 Ex vivo cultures with long duration of autologous stem cells may result in altered immunogenicity, which could lead to rejection of the cells upon transplantation. 71,72 This is likely because the self no longer recognizes its own cell once it has been immunogenically changed.…”
Section: Limitationsmentioning
confidence: 99%
“…It is even possible that avoiding the tissue-culture phase improves the efficacy of engraftment into the myocardium, as was previously reported in skeletal muscle. 30,31 Are noncultured cell muscle preparations more potent than cultured muscle cells? Which method yields the greatest number of implanted cells in vivo?…”
Section: Discussionmentioning
confidence: 99%