Exposure to toxic concentrations of aluminum activates a MAPK-like protein in cell suspension cultures of Coffea arabica

Arroyo-Serralta, Gabriela A.; Kú-González, Ángela; Hernández‐Sotomayor, S.M. Teresa; Zúñiga-Aguilar, José Juan

doi:10.1016/j.plaphy.2004.12.003

Cited by 36 publications

(22 citation statements)

References 43 publications

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“…1a, there is a biphasic dose-response curve of Al 3+ inhibition on PA formation. Indeed, a peak at 50 M AlCl 3 has been found in several reports from our group (Martínez-Estévez et al 2003;Arroyo-Serralta et al 2005). This response may be related with a signaling for cell death, since this concentration has been shown to be the lethal dose in C. arabica cells at 14 days of culture in the presence of AlCl 3 (Martínez-Estévez et al 2001).…”

Section: Discussionmentioning

confidence: 51%

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Aluminum inhibits phosphatidic acid formation by blocking the phospholipase C pathway

Ramos-Díaz

Brito‐Argáez

Munnik

et al. 2006

Planta

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Aluminum (Al(3+)) has been recognized as a main toxic factor in crop production in acid lands. Phosphatidic acid (PA) is emerging as an important lipid signaling molecule and has been implicated in various stress-signaling pathways in plants. In this paper, we focus on how PA generation is affected by Al(3+) using Coffea arabica suspension cells. We pre-labeled cells with [(32)P]orthophosphate ((32)Pi) and assayed for (32)P-PA formation in response to Al(3+). Treating cells for 15 min with either AlCl(3) or Al(NO(3))(3) inhibited the formation of PA. In order to test how Al(3+) affected PA signaling, we used the peptide mastoparan-7 (mas-7), which is known as a very potent stimulator of PA formation. The Al(3+) inhibited mas-7 induction of PA response, both before and after Al(3+) incubation. The PA involved in signaling is generated by two distinct phospholipid signaling pathways, via phospholipase D (PLD; EC: 3.1.4.4) or via Phospholipase C (PLC; EC: 3.1.4.3), and diacylglycerol kinase (DGK; EC 2.7.1.107). By labeling with (32)Pi for short periods of time, we found that PA formation was inhibited almost 30% when the cells were incubated with AlCl(3) suggesting the involvement of the PLC/DGK pathway. Incubation of cells with PLC inhibitor, U73122, affected PA formation, like AlCl(3) did. PLD in vivo activation by mas-7 was reduced by Al(3+). These results suggest that PA formation was prevented through the inhibition of the PLC activity, and it provides the first evidence for the role of Al toxicity on PA production.

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Section: Discussionmentioning

confidence: 51%

“…Recently, Al 3+ has been shown to activate a MAPKlike protein in coVee cells (Arroyo-Serralta et al 2005). Protein phosphorylation via a related MAPK is required for the signal transduction that precedes the organic acid eZux (Osawa and Matsumoto 2001).…”

Section: Discussionmentioning

confidence: 99%

Aluminum inhibits phosphatidic acid formation by blocking the phospholipase C pathway

Ramos-Díaz

Brito‐Argáez

Munnik

et al. 2006

Planta

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“…It has been documented that mitogen-activated protein kinases (MAPKs) can perceive the changes of ROS content in signal transmission for a range of stresses 23 and the distinct MAPK pathways involve specifically to excess particular metal ions. [24][25][26] ROS induced by Cu triggers SIMK and SAMK activation via SIMKK, whereas Cd activates MMK2, MMK3 besides SIMK and SAMK. 24 Recently, MPK3 and MPK6 are also evidenced to be activated by Cd-induced ROS accumulation in Arabidopsis plants.…”

Section: Interactions Between Ros and Auxin Signaling In Plant Responmentioning

confidence: 99%

“…25 A similar effect is also shown in plant response to Al stress, in which a MAPKlike protein is modulated by toxic concentrations of aluminum. 26 …”

Section: Interactions Between Ros and Auxin Signaling In Plant Responmentioning

confidence: 99%

Role of ROS and auxin in plant response to metal-mediated stress

Yuan

Liu

Jin

et al. 2013

Plant Signaling & Behavior

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“…[4][5][6] Plants showing symptoms of aluminum toxicity are more sensitive to the changes in the environmental conditions, 6 and this can be caused by aluminum-mediated effects on the signal transduction cascades. [7][8][9] One of the main components of the phospholipid signaling are the polyphosphoinositides (PPI), which are involved in several processes of cellular signalling caused by different types of stress, such are saline stress, which causes a fast increase in the phosphatidylinositol 4,5-bisphosphate (PIP 2 ) levels. 10 In addition, PIP 2 have a crucial function in several processes of cellular signaling.…”

Section: Introductionmentioning

confidence: 99%