Exposure to Vinorelbine Inhibits in Vitro Proliferation and Invasiveness of Transitional Cell Bladder Carcinoma

Bonfil, R. Daniel; Russo, Daniela M.; Schmilovich, A.

doi:10.1016/s0022-5347(01)65917-2

Cited by 12 publications

(7 citation statements)

References 15 publications

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“…Tumor cell invasion assay was carried out in 12-Ìm-pore Transwell TM cell culture chamber inserts (Corning Costar, Cambridge, Mass., USA) as previously described [5]. Briefly, the upper surface of the polycarbonate membrane was coated with 50 Ìg/100 Ìl of cold reconstituted basement membrane Matrigel ® (Collaborative Biomedical Products, Bedford, Md., USA).…”

Section: Chemoinvasion Assaymentioning

confidence: 99%

Studies on the Mechanisms Responsible for Inhibition of Experimental Metastasis of B16-F10 Murine Melanoma by Pentoxifylline

et al. 1999

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Pentoxifylline (PTX), a methylxanthine derivative widely used as a hemorheological agent in the treatment of peripheral vascular disease, was studied to unveil the mechanisms responsible for its inhibitory action on B16-F10 experimental metastasis. In vitro pretreatment of B16-F10 cells with noncytotoxic concentrations of PTX significantly inhibited their adhesion to reconstituted basement membrane Matrigel^® and type IV collagen as well as the relative activity of secreted 92 kD metalloproteinase. However, PTX pretreatment of B16-F10 cells did not affect their in vitro invasiveness. Heterotypic organ adhesion assays carried out with B16-F10 cells and suspended organ tissues demonstrated that pretreatment with noncytotoxic concentrations of PTX of both, tumor cells or lung tissue, brought about a dose-dependent inhibition of melanoma cell adhesion to lung. Immunohistochemical studies using antibodies against CD31 adhesion molecule (PECAM-1) revealed that B16-F10 cells adhere to lung endothelial cells. Our results suggest that PTX may exert its inhibitory effect on tumor lodgment, and as a consequence of that on experimental metastases, through an inhibitory action on cell adhesion molecules.

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Section: Chemoinvasion Assaymentioning

confidence: 99%

Studies on the Mechanisms Responsible for Inhibition of Experimental Metastasis of B16-F10 Murine Melanoma by Pentoxifylline

et al. 1999

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“…The anti-proliferative effect of vinorelbine against different human cell lines has already been established [23][24][25]. To verify its effect on renal cancer cell growth, A498 and 786-O cells were treated with increasing concentrations of vinorelbine (10 nM, 100 nM, and 1M) and cell survival was assessed with the MTT assay.…”

Section: Inhibition Of Cell Growthmentioning

confidence: 99%

“…Invasion of tumour cells through the matrix of the microenvironment is an early process of metastasis. It was reported that exposure to vinorelbine inhibits in vitro invasiveness of transitional cell bladder carcinoma [23]. To determine whether vinorelbine plays an inhibitory role in the invasion of renal cancer cells, we performed a Matrigel invasion assay in the presence or absence of the drug.…”

Section: Effect Of Vinorelbine On Cell Invasionmentioning

confidence: 99%

VEGF neutralizing antibody increases the therapeutic efficacy of vinorelbine for renal cell carcinoma

Sinha

Dutta

Wang

et al. 2010

J Cellular Molecular Medi

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Renal cell carcinoma (RCC) is currently one of the most treatment-resistant malignancies and affects approximately three in 10,000 people. The impact of this disease produces about 31,000 new cases in the United States per year; and 12,000 people in the United States alone die from RCC annually. Although several treatment strategies have been investigated for RCC, this cancer continues to be a therapeutic challenge. For this reason, the aim of our study is to develop a more effective combinational therapy to treat advanced RCC. We examined the effect of vinorelbine on the signalling pathways of two human renal cancer cell lines (A498 and 786-O) and also examined the in vivo effect of vinorelbine treatment alone and vinorelbine in combination with the anti-VEGF antibody 2C3 on A498 and 786-O tumour growth in nude mice. Tumour angiogenesis was measured by vWF staining, and apoptosis was determined by the TUNEL assay. We observed a significant tumour growth inhibition when using a combinational therapy of anti-VEGF antibody 2C3 and vinorelbine in both A498 and 786-O tumour-bearing mice. The results suggest a breakthrough treatment for advanced RCC.

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“…The ability of L-TACB cells to migrate towards a chemoattractant was studied using transwell chambers (Costar, Cambridge, Mass., USA) with 8-Ìm pore polycarbonate filters, coated with 5 Ìg/100 Ìl type IV collagen (Collaborative Research, Bedford, Mass., USA), as previously decribed [22,23].…”

Section: Motility and Invasion Assaysmentioning

confidence: 99%

Modulation of the Antimetastatic Effect of a Single Low Dose of Cyclophosphamide on Rat Lymphoma

et al. 1998

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The aim of this paper was to identify the mechanism/s responsible of the antimetastatic effect of a single low dose of cyclophosphamide (Cy), previously demonstrated by us in the rat lymphoma L-TACB. No direct cytotoxic antimetastatic activity of Cy could be proved. In vitro treatment of L-TACB cells with mafosfamide did not alter their invasiveness or their motility. The adoptive transfer of splenocytes from Cy-treated tumor-bearing rats, together with L-TACB cells inhibited their metastatic growth. The single low dose Cy treatment of T-immunodeficient nude mice did not show the antimetastatic effect on L-TACB observed in immunocompetent mice. An inhibition of the metastatic ability due to immunomodulation by Cy is proposed.

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Exposure to Vinorelbine Inhibits in Vitro Proliferation and Invasiveness of Transitional Cell Bladder Carcinoma

Cited by 12 publications

References 15 publications

Studies on the Mechanisms Responsible for Inhibition of Experimental Metastasis of B16-F10 Murine Melanoma by Pentoxifylline

Studies on the Mechanisms Responsible for Inhibition of Experimental Metastasis of B16-F10 Murine Melanoma by Pentoxifylline

VEGF neutralizing antibody increases the therapeutic efficacy of vinorelbine for renal cell carcinoma

Modulation of the Antimetastatic Effect of a Single Low Dose of Cyclophosphamide on Rat Lymphoma

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