Expresión diferencial de los genes de algunas enzimas lignocelulolíticas en biopelículas de Aspergillus niger

Villena, Gretty K.; Fujikawa, Takashi; Tsuyumu, Shinji; Gutiérrez-Correa, Marcel

doi:10.15381/rpb.v15i2.1731

Cited by 2 publications

(2 citation statements)

References 46 publications

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“…Some results obtained with A. fumigatus biofilms have been already discussed elsewhere [1]. Recently, we have presented results on an evaluation of transcriptional gene expression in A. niger ATCC 10864 biofilms developed on polyester cloth with lactose as carbon source [97]. The expression analysis of genes encoding some lignocellulolytic enzymes and some regulatory genes by means of Reverse Transcriptase-PCR showed that eng1, eglA, eglB, eglC, exo, and xynB genes (coding for endoglucanases, a cellobiohydrolase, and a xylanase, respectively) are differentially expressed in biofilm fermentation either time-related or through the production of more than a transcript as compared to A. niger grown in SCF.…”

Section: Transcriptomicsmentioning

confidence: 82%

“…The expression analysis of genes encoding some lignocellulolytic enzymes and some regulatory genes by means of Reverse Transcriptase-PCR showed that eng1, eglA, eglB, eglC, exo, and xynB genes (coding for endoglucanases, a cellobiohydrolase, and a xylanase, respectively) are differentially expressed in biofilm fermentation either time-related or through the production of more than a transcript as compared to A. niger grown in SCF. Likewise, the regulatory genes xlnR (cellulase activator) and creA (cellulase repressor) showed time-related expression patterns that were different in both fermentation systems, indicating that a different regulatory system may act in biofilms [97]. Therefore, we have measured transcript synthesis for A. niger endoglucanase A (eglA) and cellobiohydrolase B (cbhB) genes as well as cellulase regulatory genes xlnR and creA at 72 h growth as biofilms on polyester cloth and lactose as carbon source, and found 3-to 4-fold higher expression of both cellulolytic enzyme genes than in submerged cultures while lower expression levels of both regulatory genes were attained in biofilms as compared to submerged cultures as showed in Fig.…”

Section: Transcriptomicsmentioning

confidence: 99%

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Recent Advances on Filamentous Fungal Biofilms for Industrial Uses

Gutiérrez-Correa

Ludeña

Ramage

et al. 2012

Appl Biochem Biotechnol

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Industrial enzymes are produced by submerged fermentation (SF) and by solid-state fermentation (SSF) to a lesser extent. Although SSF has several advantages, its scale-up is difficult. The role of physiological and genetic properties of microorganisms growing attached to surfaces could explain the advantages of SSF. Filamentous fungi are naturally adapted to growth on surfaces and in these conditions they show a particular physiological behavior which is different from that in SF; thus, they also form biofilms. Fermentation by filamentous fungal biofilms (FFB) is a homogeneous production system within a liquid environment based on the infrastructure of the SF process with the productive efficiency of the SSF. Enzyme production levels of FFB are much higher than those obtained in SF and they are also amenable of mixed fungal cultivation. Transcriptomic and proteomic tools are used to uncover the fundamental biological issues behind FFB. Several genes encoding cellulolytic enzymes are either differentially expressed or overexpressed in FFB. Moreover, our proteomic studies of Aspergillus niger biofilms compared to SF indicate that many intracellular proteins are either differentially expressed or overexpressed. Clinically important fungi like A. fumigatus also form biofilms when they infect lungs and recent studies demonstrate same gene expression features. These results support our hypothesis of cell adhesion and its role in the new schemes for improved fermentative production of industrial enzymes.

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Section: Transcriptomicsmentioning

confidence: 82%

Section: Transcriptomicsmentioning

confidence: 99%