Express bioindication of environmental pollution with mercury using yeast fungi

Abstract

The issues of external influence on biological objects have interested mankind since ancient times. It can significantly affect vital activity of yeast cells. Their use for bioindication and quantitative determination of the rate of gas emission depending on composition of culture liquid makes it possible to use this process for bioindication of environment. In the article, on the basis of a developed machine for measuring the rate of carbon dioxide emission by yeast fungi, which makes it possible to use it in laboratory and field conditions, the values of the effect of mercury on the process of fermentation of culture medium by yeast are given. A method for express bioindication by yeast fungi of environmental pollution with mercury has been worked out. It serves as the basis for development of guidelines for identifying and controlling sources of chemical hazards during emergency response. The experiments have shown that the maximum effect on bioactivity of yeast is exerted by the HgCl2 salt with different concentrations, which leads to a decrease in the rate of CO2 emission and deterioration of yeast vital functions.