Express ELISA for Detection of Mortalin

Abstract

Mortalin is a widely studied stress chaperone that plays a significant role in diseases such as cancer, diabetes mellitus, liver cirrhosis, neurodegeneration and generalized aging. Based on these, the level of mortalin expression has been predicted to be an important and valuable diagnostic and prognostic marker. Conventional methods of protein analyses, such as Western blotting, immunohistochemistry or ELISA with antibodies provide specific, sensitive and useful outcomes. However, they are limited by lengthy and time-consuming protocols. Here, we present an upgrade to the existing ELISA techniques. We have prepared a conjugate of anti-mortalin antibody and luciferase enzyme that can be recruited for rapid (∼3 h) and quantitative detection of mortalin expression in a given biological sample.

Advances on Hormones in Cosmetics: Illegal Addition Status, Sample Preparation, and Detection Technology

“…Although the above methods have high sensitivity and accuracy, they are high cost, require professional operators and complicated pretreatment technologies, and cannot meet the demand for on-site and rapid detection of hormones in cosmetics. In this regard, many efforts have been carried out to explore rapid and reliable immunoassays, such as ELISA [ 88 , 89 ] and LFIA technologies [ 90 ], which could act as important supplements for the current analytical means.…”