Expressão da E-caderina em carcinoma de células escamosas e no tumor de células basais de cães

João, Carolina Franchi; Tinucci‐Costa, Mirela; Cardilli, Diogo José; Faria, João Bosco; Magalhães, Geórgia Modé; Alessi, Antônio Carlos

doi:10.1590/s0103-84782011000900020

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…Twenty samples of penile squamous cell carcinoma were subjected to immunohistochemical analysis. All immunohistochemical procedures were performed as previously described by João et al (10). SCC slides were incubated for eighteen hours with rabbit polyclonal anti-Caspase-3 antibodies diluted 1:400 (code IM0035, company Rheabiotec, São Paulo, Campinas, Brazil) and anti-E-cadherin diluted 1:100 (code IM0066, company Rheabiotec, São Paulo, Campinas, Brazil), washed and incubated for one hour with SM802 EnVision ™ (code K4065, DakoCytomation, Santa Clara, USA).…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

Immunohistochemical analysis of E-cadherin and Caspase-3 expression in equine penile squamous cell carcinoma

Nascimento,

Pinheiro,

Momo

2024

BJVP

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Neoplasms are an important cause of morbidity and mortality in horses, with squamous cell carcinoma (SCC), also called squamous cell carcinoma, being the most common genital malignant tumor in the species and the most common neoplasm in horses in the State of São Paulo. Neoplasms frequently infiltrate the corpus cavernosum and can cause metastases in regional lymph nodes, generally having a guarded to poor prognosis due to local invasion and recurrence. Given the importance of the disease, the objective was to verify whether there is a difference in the expression pattern of immunomarkers of apoptosis and cell adhesion, according to the degree of cellular differentiation of the neoplasms. Twenty equine penile SCC samples from the Animal Pathology Service of the Veterinary Hospital of the Faculty of Veterinary Medicine and Animal Science of the University of São Paulo were histologically analyzed and classified according to their degree of differentiation. In addition, they were also subjected to the immunohistochemistry technique, with the immunomarkers Caspase-3 and E-cadherin. Data were analyzed using Kendall’s correlation and the Mann-Whitney test. It was found that there is a positive correlation between the expression of immunomarkers and that there is no statistically significant difference in the expression of immunomarkers according to the degree of differentiation.

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Section: Immunohistochemical Analysismentioning

confidence: 99%

Immunohistochemical analysis of E-cadherin and Caspase-3 expression in equine penile squamous cell carcinoma

Nascimento,

Pinheiro,

Momo

2024

BJVP

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“…Down-regulation of CDH1 is linked to increased potential of tumor invasiveness and distant metastasis. The frequencies of CDH1 promoter hypermethylation appear to be correlated with advanced stage of cutaneous SCC in both dog and human (João et al, 2011).…”

Section: General and Useful Markersmentioning

confidence: 96%

“…group (Assawawongkasem et al, 2016;Bardagi et al, 2012;Bongiovanni et al, 2009;Gama et al, 2003;João et al, 2011;Keller et al, 2007;Maiolino et al, 2000;Murakami et al, 2000;Nakaichi et al, 2007;Yan et al, 2011)…”

Section: Molecular Markers Humanunclassified

Involucrin Expression in Three-Dimentional Cell Culture and Prognostic Parameters of Canine Cutaneous Squamous Cell Carcinoma (Scc) in Canine Patients and in Ultraviolet Radiation Induced Cultured Cells

Assawawongkasem

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The objectives of this study are 1) to demonstrate the involucrin (INV) expression and prognostic parameters in normal skin and cutaneous squamous cell carcinoma (CSCC), 2) to develop the artificial epidermis in dog and 3) to study the effect of INV expression on pathogenesis of ultraviolet radiation induced CSCC in dog.The first experiment is to determine the protein expression by Immunofluorescence technic (IFC) and mRNA expression of INV,Cytokeratin 10 (CK10), Ki67 and p53 normal skin(n=6) and CSCC (n=6) in dog.The protein expression pattern was demonstrated that INV and CK10 were positive in intra-cytoplasmic pattern in nucleated epidermis. While, intra-nuclear pattern was positive with Ki67 but was negative for p53. The levels of the mRNA expression of INV, CK10, Ki67, and p53 in the normal skin and CSCC in dog are in correspondence with IFC that INV of normal skin revealed more than CSCC, on the contraryKi67 in normal skin was less than CSCC with statistically difference (p<0.05). The papilloma virus was negative in all samples. Though, p53 has not shown any statistically difference (p>0.05), and the mRNA expression in CSCC tends to be more than in normal skin.The second experiment is the cultured commercial canine keratinocyte cell line (CPEK, C CELLnTEC Advanced Cell Systems, Switzerland) was successfully conducted by producing the 3 dimensional (3D) epidermal skin appearance under air-liquid interface technique. The 3D cell culture showed the epidermal characteristic similar to the epidermis on 14 days cultured. For IFC, the INV, CK10 revealed positive result while ki67 and p53 showed negative result. The mRNA expression, there were no statistical difference of INV, CK10 between 3D cultured CPEK and normal skin (p>0.05). The p53 and ki67 were negative in 3D cell cultured. In the same cultured condition, the primary cell culture both normal skin and CSCC could not be obtained good yields. The third experiment was designed siRNA for INV by online program ((https://us.bioneer.com/sirna/custom-sirna-ex.aspx), the 3 sets; INV-1, INV-2 and INV-3 could be obtained. The treatment of cultured CPEK with INV-1 siRNA for INV knockdown showed the best result among 3 sets in the reduction of mRNA levels. From the titration, 300 pmol demonstrated the highly effective with minimum cytoxicity to cultured cells. The mRNA expression of INV started to decrease with statistical difference (p<0.05) at 0 h after 5 h transfection and slightly increasing at 48 h. The cytologic appearance of cultured CPEK were initially observed after 300 mJ/cm2 of UVB irradiation exposure, at 6 h. The cell showed a number of dead and sloughing cells in the siRNA transfected with UVB irradiation group in comparison to control group. The mRNA expression of wild type p53 of cultured cell was increased after 6 h exposure of 300 mJ/cm2 of UVB and gradually increased its expression when increasing exposure time respectively. After the cells were harvested at 24 h, the results of p53 mRNA expression were statistically difference increased in cultured cell both; with and without siRNA transfection under UVB irradiation groups more than without UVB irradiation groups (p<0.05). There was no statistically difference of INV, CK10, and Ki67 expression between with and without UVB irradiation groups. The obtained results demonstrated that the protein expression by IFC and the level of mRNA expression of INV are difference between normal skin and SCC in dog. The 3D cultured CPEK could be developed as an artificial epidermis in dog. Finally, this is the first report on the design of siRNA transfected in the cultured CPEKfor INV?s knockdown. It is suggested that INV doesn?t effect on the induced in cultured keratinocyte by the UVB irradiation at 300 mJ/cm2, which is the major cause of CSCC in dog.

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Aberrant Expression of E-cadherin/β-catenin During Epidermal Tumourigenesis in Dogs

Ressel

Massone

Barbeito

2020

Journal of Comparative Pathology

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Expressão da E-caderina em carcinoma de células escamosas e no tumor de células basais de cães

Abstract: RESUMO As caderinas compreendem uma classe de moléculas de adesão celular expressa na superfície de todas as camadas epidérmicas. A E-caderina é a principal caderina

Cited by 4 publications

References 20 publications

Immunohistochemical analysis of E-cadherin and Caspase-3 expression in equine penile squamous cell carcinoma

Immunohistochemical analysis of E-cadherin and Caspase-3 expression in equine penile squamous cell carcinoma

Involucrin Expression in Three-Dimentional Cell Culture and Prognostic Parameters of Canine Cutaneous Squamous Cell Carcinoma (Scc) in Canine Patients and in Ultraviolet Radiation Induced Cultured Cells

Aberrant Expression of E-cadherin/β-catenin During Epidermal Tumourigenesis in Dogs

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