Expressed Sequence Tags of Chinese Cabbage Flower Bud cDNA

Lim, Chae Oh; Kim, Ho Yeon; Kim, Min Gab; Lee, Soo In; Chung, Woo Sik; Park, Sung Han; Hwang, IH; Cho, Moo Je

doi:10.1104/pp.111.2.577

Cited by 74 publications

(43 citation statements)

References 25 publications

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“…In addition to the demonstration of homologous sequences in Antirrhinum described here, related proteins have been identified in Betula verrucosa (Engel et al, 1997;Twardosz et al, 1997), Olea europaea (Batanero et al, 1997), Artemisia vulgaris (Engel et al, 1997), Cynodon dactylon (Knox et al, 1992), Phleum pratense, and Lilium longiflorum (Engel et al, 1997;Twardosz et al, 1997), and multiple types were identified in Brassica rapa and B. napus (Toriyama et al, 1995;Lim et al, 1996) during the course of this study. The partial amino acid sequence from the second pollen protein purified in this study, PP-A, indicates that it, too, is a member of this protein family.…”

Section: Discussionsupporting

confidence: 59%

Characterization and Immunolocalization of a Cytosolic Calcium-Binding Protein from Brassica napus and Arabidopsis Pollen1

Rozwadowski

Zhao

Jackman³

et al. 1999

Plant Physiology

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Two low-molecular-weight proteins have been purified from Brassica napus pollen and a gene corresponding to one of them has been isolated. The gene encodes an 8.6-kD protein with two EFhand calcium-binding motifs and is a member of a small gene family in B. napus. The protein is part of a family of pollen allergens recently identified in several evolutionarily distant dicot and monocot plants. Homologs have been detected in Arabidopsis, from which one gene has been cloned in this study, and in snapdragon (Antirrhinum majus), but not in tobacco (Nicotiana tabacum). Expression of the gene in B. napus was limited to male tissues and occurred during the pollen-maturation phase of anther development. Both the B. napus and Arabidopsis proteins interact with calcium, and the potential for a calcium-dependent conformational change was demonstrated. Given this affinity for calcium, the cloned genes were termed BPC1 and APC1 (B. napus and Arabidopsis pollen calcium-binding protein 1, respectively). Immunolocalization studies demonstrated that BPC1 is found in the cytosol of mature pollen. However, upon pollen hydration and germination, there is some apparent leakage of the protein to the pollen wall. BPC1 is also concentrated on or near the surface of the elongating pollen tube. The essential nature of calcium in pollen physiology, combined with the properties of BPC1 and its high evolutionary conservation suggests that this protein plays an important role in pollination by functioning as a calcium-sensitive signal molecule.

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Section: Discussionsupporting

confidence: 59%

Characterization and Immunolocalization of a Cytosolic Calcium-Binding Protein from Brassica napus and Arabidopsis Pollen1

Rozwadowski

Zhao

Jackman³

et al. 1999

Plant Physiology

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“…A full-length cDNA clone encoding a putative type IIB Ca 2 ϩ -ATPase was isolated from a soybean hypocotyl cDNA library by using a partial cDNA clone (F955) that encodes another putative Ca 2 ϩ pump from Brassica (Lim et al, 1996). For this gene, named SCA1 , the cDNA sequence showed an open reading frame of 3042 bp that encodes a polypeptide (SCA1p) with a molecular mass of 110.4 kD (Figure 1).…”

Section: Primary Structure Of Sca1pmentioning

confidence: 99%

“…The F955 clone was a partial cDNA that encoded a putative plasma membrane-type Ca 2ϩ -ATPase isolated from Brassica flower bud cDNA (Lim et al, 1996). Positive plaques from the primary screening were purified by two additional rounds of plaque hybridizations.…”

Section: Isolation Of Ca 2؉ -Atpase Cdna From Soybean Cdna Library Anmentioning

confidence: 99%

Identification of a Calmodulin-Regulated Soybean Ca²⁺-ATPase (SCA1) That Is Located in the Plasma Membrane

et al. 2000

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Ca 2 ؉ -ATPases are key regulators of Ca 2 ؉ ion efflux in all eukaryotes. Animal cells have two distinct families of Ca 2 ؉ pumps, with calmodulin-stimulated pumps (type IIB pumps) found exclusively at the plasma membrane. In plants, no equivalent type IIB pump located at the plasma membrane has been identified at the molecular level, although related isoforms have been identified in non-plasma membrane locations. Here, we identify a plant cDNA, designated SCA1 (for soybean Ca 2 ؉ -ATPase 1), that encodes Ca 2 ؉ -ATPase and is located at the plasma membrane. The plasma membrane localization was determined by sucrose gradient and aqueous two-phase membrane fractionations and was confirmed by the localization of SCA1p tagged with a green fluorescent protein. The Ca 2 ؉ -ATPase activity of the SCA1p was increased approximately sixfold by calmodulin ( K 1/2 ‫ف‬ 10 nM). Two calmodulin binding sequences were identified in the N-terminal domain. An N-terminal truncation mutant that deletes sequence through the two calmodulin binding sites was able to complement a yeast mutant (K616) that was deficient in two endogenous Ca 2 ؉ pumps. Our results indicate that SCA1p is structurally distinct from the plasma membrane-localized Ca 2 ؉ pump in animal cells, belonging instead to a novel family of plant type IIB pumps found in multiple subcellular locations. In plant cells from soybean, expression of this plasma membrane pump was highly and rapidly induced by salt (NaCl) stress and a fungal elicitor but not by osmotic stress. INTRODUCTIONCa 2 ϩ plays a central role as a second messenger in signal transduction of all eukaryotes (Bootman and Berrige, 1995;Clapham, 1995). The transient increase of cytosolic free Ca 2 ϩ concentration, [Ca 2 ϩ ] cyt , is correlated with a variety of external signals such as touch, temperature shift, abscisic acid, auxin, red light, fungal elicitors, salinity/drought, anoxia, gravity, gibberellic acid, cytokinin, oxidative stress, and hypoxia (Bush, 1995; Sanders et al., 1999). In turn, the increased [Ca 2 ϩ ] cyt triggers many signal transduction pathways, including the regulation of enzyme activity, ion channel activity, and gene expression, which results in diverse cellular responses (Bush, 1995). In addition to its role as a second messenger, Ca 2 ϩ also is important in regulating the processing of proteins in secretory pathways (Rudolph et al., 1989). Thus, cells require carefully regulated transport systems to control [Ca 2 ϩ ] in the cytoplasm and endomembrane compartments. Influx of Ca 2 ϩ to the cytosol occurs as a "downhill" transport through Ca 2 ϩ channels (Sanders et al., 1999). In contrast, the efflux of Ca 2 ϩ from the cytosol is mediated by two active Ca 2 ϩ transporters-Ca 2 ϩ pumps and Ca 2 ϩ /H ϩ antiporters-which are powered by ATP hydrolysis and proton motive force, respectively (Bush, 1995). In plants, Ca 2 ϩ -ATPases are believed to be a major Ca 2 ϩ transporter for the endoplasmic reticulum (ER), Golgi apparatus, vacuole, plastid inner membrane, and plasma membrane (Sander...

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“…The concept of using cDNAs as a route to expedite gene discovery was first demonstrated in the early 1980s (Putney et al 1983). Later gene discovery in most plants was done primarily by sample sequencing of expressed sequence tags (ESTs) (Lim et al 1996;Covitz et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling in pearl millet (Pennisetum glaucum L.R. Br.) for identification of differentially expressed drought responsive genes

Choudhary

Jayanand

Padaria

2015

Physiol Mol Biol Plants

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Pearl millet (Pennisetum glaucum) is an important cereal of traditional farming systems that has the natural ability to withstand various abiotic stresses. The present study aims at the identification and validation of major differentially expressed genes in response to drought stress in P. glaucum by Suppression Subtractive Hybridization (SSH) analysis. Twenty-two days old seedlings of P. glaucum cultivar PPMI741 were subjected to drought stress by treatment of 30 % Polyethylene glycol for different time periods 30 min

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Expressed Sequence Tags of Chinese Cabbage Flower Bud cDNA

Cited by 74 publications

References 25 publications

Characterization and Immunolocalization of a Cytosolic Calcium-Binding Protein from Brassica napus and Arabidopsis Pollen1

Characterization and Immunolocalization of a Cytosolic Calcium-Binding Protein from Brassica napus and Arabidopsis Pollen1

Identification of a Calmodulin-Regulated Soybean Ca²⁺-ATPase (SCA1) That Is Located in the Plasma Membrane

Transcriptional profiling in pearl millet (Pennisetum glaucum L.R. Br.) for identification of differentially expressed drought responsive genes

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Expressed Sequence Tags of Chinese Cabbage Flower Bud cDNA

Cited by 74 publications

References 25 publications

Characterization and Immunolocalization of a Cytosolic Calcium-Binding Protein from Brassica napus and Arabidopsis Pollen1

Characterization and Immunolocalization of a Cytosolic Calcium-Binding Protein from Brassica napus and Arabidopsis Pollen1

Identification of a Calmodulin-Regulated Soybean Ca2+-ATPase (SCA1) That Is Located in the Plasma Membrane

Transcriptional profiling in pearl millet (Pennisetum glaucum L.R. Br.) for identification of differentially expressed drought responsive genes

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Identification of a Calmodulin-Regulated Soybean Ca²⁺-ATPase (SCA1) That Is Located in the Plasma Membrane