Expressing a bacterial mercuric ion binding protein in plant for phytoremediation of heavy metals

Hsieh, Ju Liang; Chen, Ching-Yi; Chiu, Meng Hsuen; Chein, Mei fang; Chang, Jo Shu; Endo, Ginro; Huang, Chieh-Chen

doi:10.1016/j.jhazmat.2008.04.079

Cited by 73 publications

(17 citation statements)

References 33 publications

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“…These transgenic poplars were reported to have a much higher potential for heavy metal and pesticide detoxification as compared to wild type plants in greenhouse experiments. Another approach was based on cloning and expression of metallothionein gene, mt, from mouse (Ruiz et al 2011) and mercuric ion binding protein gene, MerP, from Bacillus megaterium MB1 (Hsieh et al 2009) in plants to enable them for accumulation of Hg. Similarly, tobacco plants engineered to express bacterial mercury transporter gene, MerT, and ppK gene for polyphosphate kinase had improved tolerance and phytoremediation for Hg (Nagata et al 2009).…”

Section: Genetic Engineering Of Plantsmentioning

confidence: 99%

Phytoremediation: recent advances in plant-endophytic synergistic interactions

et al. 2015

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Background Plant-endophyte partnership provides an excellent gateway toward restoration of contaminated ecosystems. The interactions between both partners can be manipulated by introducing novel catabolic/ metabolic genes into host plant or endophytic bacteria genomes. The potential of the engineered organisms to degrade or accumulate contaminants is much wider than their wild relatives. Scope This review covers the recent developments for engineering catabolic/metabolic genes from a wide range of sources into plants or endophytic bacteria for the development of modified plant-endophyte interactions. Genetic alteration of plants promises enhanced catabolism by plant's own enzymatic machinery or greater contaminant uptake/accumulation for subsequent in planta detoxification by complementary endophytes. On the other hand, bacteria may also be engineered to enhance the potential for degradation or alteration of catabolic pathways, either to protect the host plant against phytotoxicity or to improve the overall efficiency of phytoremediation in planta, a situation especially suitable when hydrophilic compounds fail to be degraded by rhizospheric microbes due to the rapid uptake by plants. This is followed by discussion on kinetic parameters controlling phytoremediation. Conclusions It is hypothesized that transgenic approach can result in synergistic and effective plant-endophyte partnerships for wider-range and enhanced capabilities of degrading and/or detoxifying contaminants.

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Section: Genetic Engineering Of Plantsmentioning

confidence: 99%

Phytoremediation: recent advances in plant-endophytic synergistic interactions

et al. 2015

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“…According to Kiyono et al ( 2012 ) when Arabidopsis was introduced with a bacterial merC gene from the Tn 21 -encoded mer operon resulted in more resistant to cadmium than the wild type and accumulated signifi cantly more cadmium. Similarly, transposon TnMERI1 of Bacillus megaterium strain MB1 was used to make the transgenic Arabidpsis for the expression of a specifi c mercuric ion binding protein (MerP) to increase the tolerance and accumulation capacity for mercury, cadmium, and lead (Hsieh et al 2009 ). The root-colonizing bacterium Pseudomonas fl uorescens has been engineered to express XplA gene to degrade explosive chemicals like Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) in the rhizosphere (Lorenz et al 2013 ).…”

Section: Transgenic Technology To Enhance the Phytoremediation Effi Cmentioning

confidence: 99%

Phytoremediation of Heavy Metals: The Use of Green Approaches to Clean the Environment

Singh

Santal

2015

Phytoremediation

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“…Since the interface between microbes and rhizosphere is considered to greatly influence the growth and survival of plants, alternative phytoremediation methods that exploit rhizosphere bacteria to reduce metal and organic pollutant toxicity to plants have been investigated extensively (Zhuang et al 2007;Hsieh et al 2009;Karami and Shamsuddin 2010). Bacterial diversity and the number of culturable heterotrophic bacteria decrease significantly in polyaromatic hydrocarbon-and phenyl urea herbicides-contaminated soils.…”

Section: Microbe-assisted Phytoremediationmentioning

confidence: 99%