Expressing a gene encoding wheat oxalate oxidase enhances resistance to Sclerotinia sclerotiorum in oilseed rape (Brassica napus)

Xia, Dong; Ji, Ruiqin; Guo, Xuelan; Foster, Simon J.; Chen, Hong; Dong, Caihua; Liu, Yueying; Hu, Qiong; S, Liu

doi:10.1007/s00425-008-0740-2

Cited by 134 publications

(106 citation statements)

References 30 publications

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“…The crystals are thought to deter herbivore feeding (Korth et al, 2006) and may also serve a role in regulating free calcium levels (Franceschi and Nakata, 2005). Oxalic acid is toxic when applied to plant leaves (Dong et al, 2008), and some fungal pathogens, such as Sclerotinia sclerotiorum, secrete oxalate that acts as an important virulence factor during infection. Evidence indicates that oxalate may stimulate stomatal opening, interfere with cell wall structure, induce low pHactivated pectolytic enzymes and act as an elicitor of programmed cell death (Bateman and Beer, 1965;Lumsden, 1976;Guimarã es and Stotz, 2004;Hegedus and Rimmer, 2005;Dong et al, 2008;Kim et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Oxalic acid is toxic when applied to plant leaves (Dong et al, 2008), and some fungal pathogens, such as Sclerotinia sclerotiorum, secrete oxalate that acts as an important virulence factor during infection. Evidence indicates that oxalate may stimulate stomatal opening, interfere with cell wall structure, induce low pHactivated pectolytic enzymes and act as an elicitor of programmed cell death (Bateman and Beer, 1965;Lumsden, 1976;Guimarã es and Stotz, 2004;Hegedus and Rimmer, 2005;Dong et al, 2008;Kim et al, 2008). S. sclerotiorum mutants deficient in oxalate synthesis have greatly reduced infectivity (Godoy et al, 1990;Guimarã es and Stotz, 2004).…”

Section: Introductionmentioning

confidence: 99%

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A Previously Unknown Oxalyl-CoA Synthetase Is Important for Oxalate Catabolism in Arabidopsis

et al. 2012

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Oxalate is produced by several catabolic pathways in plants. The best characterized pathway for subsequent oxalate degradation is via oxalate oxidase, but some species, such as Arabidopsis thaliana, have no oxalate oxidase activity. Previously, an alternative pathway was proposed in which oxalyl-CoA synthetase (EC 6.2.1.8) catalyzes the first step, but no gene encoding this function has been found. Here, we identify ACYL-ACTIVATING ENZYME3 (AAE3; At3g48990) from Arabidopsis as a gene encoding oxalyl-CoA synthetase. Recombinant AAE3 protein has high activity against oxalate, with K m = 149.0 6 12.7 mM and V max = 11.4 6 1.0 mmol/min/mg protein, but no detectable activity against other organic acids tested. Allelic aae3 mutants lacked oxalyl-CoA synthetase activity and were unable to degrade oxalate into CO 2 . Seeds of mutants accumulated oxalate to levels threefold higher than the wild type, resulting in the formation of oxalate crystals. Crystal formation was associated with seed coat defects and substantially reduced germination of mutant seeds. Leaves of mutants were damaged by exogenous oxalate and more susceptible than the wild type to infection by the fungus Sclerotinia sclerotiorum, which produces oxalate as a phytotoxin to aid infection. Our results demonstrate that, in Arabidopsis, oxalylCoA synthetase encoded by AAE3 is required for oxalate degradation, for normal seed development, and for defense against an oxalate-producing fungal pathogen.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Previously Unknown Oxalyl-CoA Synthetase Is Important for Oxalate Catabolism in Arabidopsis

et al. 2012

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“…The plant suff ers from several diseases which result in the reduction of the yield and quality of production (1). Sclerotinia stem rot caused by Sclerotinia sclerotiorum is the second most damaging disease of the oilseed rape yield worldwide, that infects agro-economically crops such as sunfl ower (Helianthus annuus), soybean (Glycine max) and oilseed (Brassica napus) (2)(3).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-Mediated Transformation of the Oryza sativa Thaumatin-Like Protein to Canola (R Line Hyola308) for Enhancing Resistance to Sclerotinia sclerotiorum

Aghazadeh

Zamani

Motallebi

et al. 2017

Iran. J. Biotechnol.

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Background:Canola is an agro-economically oilseed crop. Yield loss due to fungal disease of stem rot caused by Sclerotinia sclerotiorum is a serious problem in canola cultivation. Thaumatin-like proteins are large groups of the pathogenesis-related proteins which provide resistance to the fungal infection in response to invading pathogens and play a key role in plant defense system. Objectives: Transformation of the rice tlp into canola via Agrobacterium-mediated transformation and evaluation of the antifungal activity of the expressed TLP in the transgenic events on the S. sclerotiorum growth was subject to investigation. Materials and methods:The canola (R line Hyola308) was used for transformation experiment. The vector, pBITLPRA1, was used for the stable transformation. The PCR and southern blotting techniques were used to confi rm transgene's presence in the transgenic canola events. Antifungal activity of transgenic plants was evaluated by the radial diff usion and spore germination assays. T 2 transgenic plants were evaluated by the intact leaf inoculation method in greenhouse assay. Results: In this study, pBITLPRA1 construct containing tlp gene was introduced into canola and the transformed plants were verifi ed by PCR. The glucanase activity of tlp gene in T 0 generation was measured and transgenic plants with high activity were assessed by Southern blot analysis to confi rm the copy number of the gene. Also, antifungal activity of the single copy T 0 transgenic plants against Sclerotinia sclerotiorum was evaluated by radial diff usion and spore germination assays. In greenhouse assay, evaluation of T 2 transgenic plants by the intact leaf inoculation method demonstrated that following the infection with S. sclerotiorum, there was a signifi cant reduction in the lesion's diameter in transgenic lines compared to the non-transgenic ones. Conclusions: These results revealed that expression of TLP has an inhibitory eff ect against fungus compared to nontransgenic plants both in vitro and in vivo (i.e., greenhouse condition). These transgenic lines could be used as the additional sources of disease resistance for canola breeding program.

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“…No caso específico do mofo branco, o controle biológico mais conhecido utilizado é o fungo do gênero Trichoderma (Geraldine et al, 2013) (Kesarwani et al, 2000;Donaldson et al, 2001;Livingstone et al, 2005;Dias et al, 2006;Dong et al, 2008;Walz et al, 2008;. Outra alternativa é o uso da estratégia de silenciamento gênico induzido pelo hospedeiro (explicada detalhadamente mais a frente), a qual tem sido utilizada para alcançar resistência a fungos biotróficos, tais como do Erysiphe blumeria em cevada e trigo , Puccinia striiformis no trigo , Puccinia triticina no trigo , Fusarium oxysporum em banana e Bremia lactucae em alface (Govindarajulu et al, 2014).…”

Section: Mofo Brancounclassified

“…The main strategy used so far consists of the expression of genes encoding enzymes capable of degrading oxalic acid, such as oxalate oxidases and oxalate decarboxylases, which have produced enhanced resistance to S. sclerotiorum (Kesarwani et al, 2000;Dias et al, 2006;Dong et al, 2008;. Another alternative is the use of host-induced gene silencing (HIGS) methods, which exploit the silencing effect of small interfering RNA (siRNA) signals in interacting organisms, and involves host expression of siRNA-generating constructs directed against genes in associated pathogens, parasites or symbionts (Knip et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Silenciamento gênico induzido pelo hospedeiro (HIGS) do gene da quitina sintase em Sclerotinia sclerotiorum

Andrade¹

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A Deus por toda criação e por estar sempre presente em minha vida me guiando e iluminando os caminhos.Aos meus pais por todas as vezes que abdicaram de algo pelos filhos, para que possamos alcançar nossos sonhos. Muito obrigada pelo incentivo, amor e carinho para que eu conseguisse seguir em frente.Ao Francisco Aragão por sua dedicada orientação, disponibilidade, apoio, incentivo e exemplo de profissional. Agradeço todas as conversas em que, mesmo sem saber, me inspirou a me manter firme em meu objetivo.Ao meu irmão Adriano pela presença, mesmo em suas ausências, apoio constante, conselhos precisos e ajuda indispensável.À minha vó Mariana pelos ensinamentos e exemplo de vida. A minha "tia" Kaká que me recebeu tão bem em minha cidade natal quando estava só.Aos colegas de laboratório Aisy, Déia, Citadin, Serginho, Zotta, Mirella, Roberto, Natália, Pedro, Angélica, Fran, Lídia, Otacílio, Rebeca, Tomas, Lorena, Pabline. À Thaís, Maria Laine e Abdul, que me mostraram o real valor de uma amizade e que em um ambiente de trabalho amizades verdadeiras e sinceras nascem e permanecem por toda a vida e, que vale a pena viver ao lado de pessoas assim.A minha amiga Nayhanne que não foi somente uma colega, confidente, irmã, foi meu apoio (nem sempre firme), minha conselheira (nem sempre certa), mas sempre ali, para o que viesse. Uma irmã de alma que tornou esse período de doutorado mais leve e divertido. À Elsa sempre prestativa e disponível a nos ajudar.Aos meus eternos estagiários Alana e Felipe, que sem eles este trabalho não teria sido o mesmo. E com quem aprendi muito mais do que ensinei.À Universidade de Brasília, Embrapa e ao CNPq, pela concessão da bolsa de estudos.A todos os pesquisadores e funcionários da Embrapa Recursos Genéticos e Biotecnologia.Aos colegas da Dow AgroSciences que me incentivaram nesta reta final. As amigas Marcela, Tathi e Clarissa que me receberam em minha nova cidade e foram fundamentais.Às amigas de Brasília, que sempre foram minha família aqui, dos grupos Garotas de Ipanema, Manicômio e Toscanetes, me apoiando nos momentos que mais precisei.A todos meu muito obrigada! "Não importa o quanto a vida se torna cada vez mais urbana, todos nós vivemos de agricultura" (Cardoso, 1994;Vieira, 1994;Menezes, 1995 Estes trabalhos são um exemplo de como HIGS em nematoides pode ser uma boa ferramenta para ser usada no controle e estudo deste parasita tão importante para agricultura mundial. neste processo é a escolha das melhores opções para expressão dos genes nas plantas e daqueles genes vitais para o ciclo celular das células fúngicas. Vários candidatos podem ser apontados, mas resta a pergunta se seria possível HIGS com estes genes, devido à similaridade entre sequências de genes de plantas e fungos. Sumário Fungos Biotróficos, Necrotróficos e Hemibiotróficos HIGS em plantas parasitas Parede celular e QuitinaA parede celular é uma estrutura essencial que mantém a viabilidade da célula fúngica, conferindo sua morfologia típica e protegendo contra injúrias externas. A parede celular é a estrutura dete...

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Expressing a gene encoding wheat oxalate oxidase enhances resistance to Sclerotinia sclerotiorum in oilseed rape (Brassica napus)

Cited by 134 publications

References 30 publications

A Previously Unknown Oxalyl-CoA Synthetase Is Important for Oxalate Catabolism in Arabidopsis

A Previously Unknown Oxalyl-CoA Synthetase Is Important for Oxalate Catabolism in Arabidopsis

Agrobacterium-Mediated Transformation of the Oryza sativa Thaumatin-Like Protein to Canola (R Line Hyola308) for Enhancing Resistance to Sclerotinia sclerotiorum

Silenciamento gênico induzido pelo hospedeiro (HIGS) do gene da quitina sintase em Sclerotinia sclerotiorum

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