Expressing a Target Mimic of miR156fhl-3p Enhances Rice Blast Disease Resistance Without Yield Penalty by Improving SPL14 Expression

Zhang, Lingli; Li, Yan; Zheng, Yuan; Wang, He; Yang, Xue‐Mei; Chen, Jinfeng; Zhou, Shan; Wang, Liang-Fang; Li, Xupu; Ma, Xiaochun; Jun, Zhao; Pu, Mei; Feng, Hui; Fan, Jing; Zhang, Jiwei; Huang, Ying; Wang, Wenming

doi:10.3389/fgene.2020.00327

Cited by 35 publications

(41 citation statements)

References 62 publications

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“…Besides, some sRNAs also coordinate responses to biotic stressors with the regulation of growth and development. For examples, miR156-OsIPA1 module negatively regulates rice resistance to M. oryzae and it also regulates seed dormancy and germination; miR396-OsGRFs modules negatively regulate rice resistance to M. oryzae and they also regulate plant height, leaf morphology and seed size (Guo et al 2016;Tang et al 2018;Zhang et al 2018a;Zhang et al 2020). Therefore, sRNA pathway may act as a part of innate immunity to tightly coordinate rice growth, development, and immunity, thus could be used as a candidate to balance the trade-off between yield and resistance in rice breeding.…”

Section: Discussionmentioning

confidence: 99%

“…More than 70 miRNAs respond to M. oryzae or its elicitors (Li et al 2019c). Among these miRNAs, five have been identified as positive regulators in rice blast resistance, namely miR160a, miR162a, miR166k-h, miR398b, and miR7695, whereas eight have been identified as negative regulators, namely miR156, miR164a, miR167d, miR169a, miR319b, miR396, miR1873, and miR444b.2 (Chandran et al 2018;Li et al 2020a;Li et al 2019b;Li et al 2014;Li et al 2017;Salvador-Guirao et al 2018;Sanchez-Sanuy et al 2019;Wang et al 2018b;Xiao et al 2017;Zhang et al 2018b;Zhang et al 2020;Zhao et al 2020;Zhou et al 2020). Obviously, many miRNAs play a role in regulating rice-M. oryzae interactions.…”

Section: Small Rnas In Rice-m Oryzae Pathological Systemmentioning

confidence: 99%

“…Besides the miRNA regulatory modules mentioned above, quite a few miRNA regulatory modules have been functionally characterized in rice immunity against M. oryzae (Table 1). The negative regulatory modules generally impair the accumulation of H 2 O 2 and the induction of defense-related genes in transgenic lines overexpressing miRNAs or mutants of target genes, such as miR167d-OsARF12, miR396a/c/d/h-OsGRF6/7/8/9, miR169a-OsNF-YAs, miR444b.2-OsMADS, miR1873-Os05g01790, and miR156-OsSPL14 (Chandran et al 2018;Li et al 2017;Xiao et al 2017;Zhang et al 2020;Zhao et al 2020;Zhou et al 2020). Conversely, the positive regulatory modules enhance defense responses, such as miR162a-OsDCL1 and miR166k-OsEIN2.1 modules (Li et al 2020a;Salvador-Guirao et al 2018).…”

Section: Small Rnas In Rice-m Oryzae Pathological Systemmentioning

confidence: 99%

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Contribution of Small RNA Pathway to Interactions of Rice with Pathogens and Insect Pests

Feng

Zhao

2021

Rice

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Small RNAs (sRNAs) are mainly classified into microRNAs (miRNAs) and small interfering RNAs (siRNAs) according to their origin. miRNAs originate from single-stranded RNA precursors, whereas siRNAs originate from double-stranded RNA precursors that are synthesized by RNA-dependent RNA polymerases. Both of single-stranded and double-stranded RNA precursors are processed into sRNAs by Dicer-like proteins. Then, the sRNAs are loaded into ARGONAUTE proteins, forming RNA-induced silencing complexes (RISCs). The RISCs repress the expression of target genes with sequences complementary to the sRNAs through the cleavage of transcripts, the inhibition of translation or DNA methylation. Here, we summarize the recent progress of sRNA pathway in the interactions of rice with various parasitic organisms, including fungi, viruses, bacteria, as well as insects. Besides, we also discuss the hormone signal in sRNA pathway, and the emerging roles of circular RNAs and long non-coding RNAs in rice immunity. Obviously, small RNA pathway may act as a part of rice innate immunity to coordinate with growth and development.

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Section: Discussionmentioning

confidence: 99%

Section: Small Rnas In Rice-m Oryzae Pathological Systemmentioning

confidence: 99%

Section: Small Rnas In Rice-m Oryzae Pathological Systemmentioning

confidence: 99%

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Contribution of Small RNA Pathway to Interactions of Rice with Pathogens and Insect Pests

Feng

Zhao

2021

Rice

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“…Previous studies revealed that a substantial fraction of the miRNAome is regulated during M. oryzae infection, and/or treatment with M. oryzae elicitors in rice plants (Baldrich et al., 2015 ; Campo et al., 2013 ; Li et al., 2016 ). However, only few miRNAs with a function in blast resistance have been reported, which include positive (miR7695, miR160, miR162, miR398 and miR166k‐166h) and negative (miR156, miR164, miR167, miR169, miR319, miR396, miR442 and miR1873) regulators of immune responses (Campo et al., 2013 ; Chandran et al., 2019 ; Li et al., 2014 , 2017 , 2020b ; Salvador‐Guirao et al., 2018b ; Sánchez‐Sanuy et al., 2019 ; Wang et al., 2018 ; Zhang et al., 2018b , 2020 ; Zhao et al., 2020 ; Zhou et al., 2019 ). Clearly, the involvement of miRNAs in rice immunity has only begun to emerge.…”

Section: Introductionmentioning

confidence: 99%

A novel Transposable element‐derived microRNA participates in plant immunity to rice blast disease

Campo

Sánchez-Sanuy

Camargo-Ramírez

et al. 2021

Plant Biotechnology Journal

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Summary MicroRNAs (miRNAs) are small non‐coding RNAs that direct post‐transcriptional gene silencing in plant development and stress responses through cleavage or translational repression of target mRNAs. Here, we report the identification and functional characterization of a new member of the miR812 family in rice (named as miR812w) involved in disease resistance. miR812w is present in cultivated Oryza species, both japonica and indica subspecies, and wild rice species within the Oryza genus, but not in dicotyledonous species. miR812w is a 24nt‐long that requires DCL3 for its biogenesis and is loaded into AGO4 proteins. Whereas overexpression of miR812w increased resistance to infection by the rice blast fungus Magnaporthe oryzae, CRISPR/Cas9‐mediated MIR812w editing enhances disease susceptibility, supporting that miR812w plays a role in blast resistance. We show that miR812w derives from the Stowaway type of rice MITEs (Miniature Inverted‐Repeat Transposable Elements). Moreover, miR812w directs DNA methylation in trans at target genes that have integrated a Stowaway MITE copy into their 3′ or 5′ untranslated region (ACO3, CIPK10, LRR genes), as well as in cis at the MIR812w locus. The target genes of miR812 were found to be hypo‐methylated around the miR812 recognition site, their expression being up‐regulated in transgene‐free CRISPR/Cas9‐edited miR812 plants. These findings further support that, in addition to post‐transcriptional regulation of gene expression, miRNAs can exert their regulatory function at the transcriptional level. This relationship between miR812w and Stowaway MITEs integrated into multiple coding genes might eventually create a network for miR812w‐mediated regulation of gene expression with implications in rice immunity.

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“…Overexpression of miR812w enhances resistance by regulating the methylation of target genes ( Campo et al, 2021 ). In contrast, miR156 ( Zhang et al, 2020 ), miR159 ( Chen et al, 2021 ), miR164a ( Wang Z. Y. et al, 2018 ), miR167 ( Zhao et al, 2019 ), miR168 ( Wang et al, 2021 ), miR169 ( Li et al, 2017 ), miR319b ( Zhang et al, 2018 ), miR396 ( Chandran et al, 2019 ), miR439 ( Lu et al, 2021 ), and miR1873 ( Zhou et al, 2020 ) negatively regulate rice resistance to blast fungus by suppressing the expression of their target genes.…”

Section: Introductionmentioning

confidence: 99%

Blocking miR530 Improves Rice Resistance, Yield, and Maturity

Wang

Bhutto

et al. 2021

Front. Plant Sci.

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MicroRNAs fine-tune plant growth and resistance against multiple biotic and abiotic stresses. The trade-off between biomass and resistance can penalize crop yield. In this study, we have shown that rice miR530 regulates blast disease resistance, yield, and growth period. While the overexpression of miR530 results in compromised blast disease resistance, reduced grain yield, and late maturity, blocking miR530 using a target mimic (MIM530) leads to enhanced resistance, increased grain yield, and early maturity. Further study revealed that the accumulation of miR530 was decreased in both leaves and panicles along with the increase of age. Such expression patterns were accordant with the enhanced resistance from seedlings to adult plants, and the grain development from panicle formation to fully-filled seeds. Divergence analysis of miR530 precursor with upstream 1,000-bp promoter sequence in 11 rice species revealed that miR530 was diverse in Oryza sativa japonica and O. sativa indica group, which was consistent with the different accumulation of miR530 in japonica accessions and indica accessions. Altogether, our results indicate that miR530 coordinates rice resistance, yield, and maturity, thus providing a potential regulatory module for breeding programs aiming to improve yield and disease resistance.

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Expressing a Target Mimic of miR156fhl-3p Enhances Rice Blast Disease Resistance Without Yield Penalty by Improving SPL14 Expression

Abstract: Expressing a Target Mimic of miR156fhl-3p Enhances Rice Blast Disease Resistance Without Yield Penalty by Improving SPL14 Expression. Front. Genet. 11:327.

Cited by 35 publications

References 62 publications

Contribution of Small RNA Pathway to Interactions of Rice with Pathogens and Insect Pests

Contribution of Small RNA Pathway to Interactions of Rice with Pathogens and Insect Pests

A novel Transposable element‐derived microRNA participates in plant immunity to rice blast disease

Blocking miR530 Improves Rice Resistance, Yield, and Maturity

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