Expression, Affinity, and Functional Characterization of the Specific Binding of Two Putative Pheromone-Binding Proteins in the Omnivorous German Cockroach <i>Blattella germanica</i>

Wang, Hong; Ma, Yunfeng; Wang, Meimei; Chen, Guanglei; Dewer, Youssef; He, Ming; Zhang, Fan; Yang, Yaoqing; Liu, Jianfeng; He, Peng

doi:10.1021/acs.jafc.0c02456

Cited by 21 publications

(27 citation statements)

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“…The similar antennae‐biased expression of OBPs was observed in many insect species (Zhang et al ., 2015; Paula et al ., 2016, 2018; Wei et al ., 2017; Yin et al ., 2019; Zhu et al ., 2020). Studies show that some OBPs with male‐biased expression could bind and perceive the female sex pheromones and host plant volatiles for feeding or mating (Ochieng et al ., 2002; Allen and Wanner, 2011; Li et al ., 2021; Wang et al ., 2020a, 2020b). However, the OBPs with female‐biased expression usually responded to host plant volatiles for feeding or oviposition (Leal, 1998; Zhu et al .…”

Section: Discussionmentioning

confidence: 99%

“…So far, some adult‐control strategies, including sex attractant, aggregation pheromones, oviposition lure, plant volatiles and food traps ( Ulmus pumila and Ricinus communis ) in the field, can interrupt the olfactory behaviours of host selection and mate selection (Zhang et al ., 2006; Li et al ., 2013; Rizwangul et al ., 2018; Li et al ., 2020b, c; Nyamwasa et al ., 2020). The host‐plant selection behaviour was reported to be modulated by chemical sensing of scarab beetle olfaction (Wang et al ., 2013, 2020a, 2020b; Ju et al ., 2018; Yin et al ., 2019; Wei et al ., 2020).…”

Section: Introductionmentioning

confidence: 99%

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Two classic OBPs modulate the responses of female Holotrichia oblita to three major ester host plant volatiles

Wei

Qin

Cao

et al. 2021

Insect Molecular Biology

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Insects possess a fairly sophisticated olfactory system in their antennae to detect odorants essential for their survival and reproduction. Among them, insect first perceives odour sources by odorant‐binding proteins (OBPs) to locate host‐plants. Methyl salicylate, (Z)‐3‐hexenyl acetate and dibutyl phthalate are major volatile components of Ulmus pumila and Ricinus communis and elicit strong responses of the scarab beetle Holotrichia oblita adults. However, olfactory perception of the scarab beetle to these odorant compounds is unclear. In the current study, we cloned the OBP6 and OBP7 of H. oblita. The expression pattern shows that the two genes were highly expressed in the antennae of female beetles. Binding assays verified that the HoblOBP6 had a better binding affinity to methyl salicylate, and so did HoblOBP7 to (Z)‐3‐hexenyl acetate and dibutyl phthalate. The effect on the responses of female beetles to the three compounds was decreased significantly after these two genes were silenced by RNA interference. These results indicate that HoblOBP6 and HoblOBP7 are essential for female H. oblita perception of methyl salicylate, (Z)‐3‐hexenyl acetate and dibutyl phthalate. Our study provides important insights into the olfactory mechanism of female H. oblita to ester plant volatiles and could facilitate the development of potential pest control strategies in the field.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Two classic OBPs modulate the responses of female Holotrichia oblita to three major ester host plant volatiles

Wei

Qin

Cao

et al. 2021

Insect Molecular Biology

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“…Plasmids were transformed into E. coli BL21 (DE3) to express these proteins according to the method recently described by us and other researchers (Damberger et al ., 2013; Katti et al ., 2013; Zhu et al ., 2016; Wang et al ., 2020; Zhang et al ., 2020 b , 2020 b ). After the E. coli cells were crushed by ultrasound, the recombinant AlepPBP2 and AlepPBP3 were detected to be insoluble inclusion body by SDS-PAGE analysis.…”

Section: Methodsmentioning

confidence: 99%

AlepPBP2, but not AlepPBP3, may involve in the recognition of sex pheromones and maize volatiles inAthetis lepigone

Yang

Zhang

et al. 2022

Bull. Entomol. Res.

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Athetis lepigone Möschler (Lepidoptera, Noctuidae) is a common maize pest in Europe and Asia. However, there is no long-term effective management strategy is available yet to suppress its population. Adults rely heavily on olfactory cues to locate their optimal host plants and oviposition sites. Pheromone-binding proteins (PBPs) are believed to be responsible for recognizing and transporting different odorant molecules to interact with receptor membrane proteins. In this study, the ligand-binding specificities of two AlepPBPs (AlepPBP2 and AlepPBP3) for sex pheromone components and host plant (maize) volatiles were measured by fluorescence ligand-binding assay. The results demonstrated that AlepPBP2 had a high affinity with two pheromones [(Z)-7-dodecenyl acetate, Ki = 1.11 ± 0.1 μM, (Z)-9-tetradecenyl acetate, Ki = 1.32 ± 0.15 μM] and ten plant volatiles, including (-)-limonene, α-pinene, myrcene, linalool, benzaldehyde, nonanal, 2-hexanone, 3-hexanone, 2-heptanone and 6-methyl-5-hepten-2-one. In contrast, we found that none of these chemicals could bind to AlepPBP3. Our results clearly show no significant differences in the functional characterization of the binding properties between AlepPBP2 and AlepPBP3 to sex pheromones and host plant volatiles. Furthermore, molecular docking was employed for further detail on some crucial amino acid residues involved in the ligand-binding of AlepPBP2. These findings will provide valuable information about the potential protein binding sites necessary for protein-ligand interactions which appear as attractive targets for the development of novel technologies and management strategies for insect pests.

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“…The communication of the insect chemosensory system is characterized by stability, sensitivity and specificity 7–9 . First, hydrophobic odorants in the environment enter the lymph through the olfactory pores of insect cuticular chemosensilla 10, 11 . Second, odorant binding proteins (OBPs) 12, 13 and chemosensory proteins (CSPs) 14, 15 which are soluble proteins containing a hydrophobic pocket bind to hydrophobic odorants and transport them to the surface of olfactory sensory neurons (OSNs).…”

Section: Introductionmentioning

confidence: 99%

Three chemosensory proteins from the sweet potato weevil, Cylas formicarius, are involved in the perception of host plant volatiles

Hua

Zhou

et al. 2021

Pest Management Science

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BACKGROUND Chemosensory proteins (CSPs) play important roles in chemical communication, but their precise physiological functions are still unclear. Cylas formicarius is the most serious pest attacking sweet potato around the world. At present, there is no effective way to control this pest. RESULTS Our results showed that CforCSP1, 5 and 6 genes were highly expressed in the antennae of both sexes of C. formicarius. In addition, results from a fluorescence competitive binding assay showed that the CforCSP1, 5 and 6 proteins had high binding affinities for 17 plant volatiles including eight host plant volatiles. This indicated that the three proteins may be involved in the detection of host plant volatiles. Furthermore, results from four‐arm olfactometer bioassays showed that there was a significant tendency for C. formicarius to be attracted to eucalyptol, β‐carotene, benzaldehyde, vanillin and phenethyl alcohol, while it was repelled by β‐ionone. Finally, the levels of expression of the three CforCSPs in C. formicarius were successfully inhibited by RNA interference (RNAi). Behavioral experiments showed that CforCSP1, 5 and 6‐deficient C. formicarius were partly anosmic to β‐cyclocitral, benzaldehyde, octyl aldehyde, and β‐ionone and exhibited a reduced ability to locate the host plant volatiles β‐carotene and vanillin. CONCLUSION Our data suggest that CforCSP1, 5 and 6 likely are involved in the chemical communication between C. formicarius and host plant volatiles, which may play pivotal roles in oviposition and feeding site preferences. More importantly, these results could provide information for the development of monitoring and push‐pull strategies for the control of C. formicarius. © 2021 Society of Chemical Industry.

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Expression, Affinity, and Functional Characterization of the Specific Binding of Two Putative Pheromone-Binding Proteins in the Omnivorous German Cockroach Blattella germanica

Cited by 21 publications

References 50 publications

Two classic OBPs modulate the responses of female Holotrichia oblita to three major ester host plant volatiles

Two classic OBPs modulate the responses of female Holotrichia oblita to three major ester host plant volatiles

AlepPBP2, but not AlepPBP3, may involve in the recognition of sex pheromones and maize volatiles inAthetis lepigone

Three chemosensory proteins from the sweet potato weevil, Cylas formicarius, are involved in the perception of host plant volatiles

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