Expression analysis of agglutinin-like sequence 1 of Candida albicans

Ibrahim, Ashraf S.; Sheppard, Don; Fu, Yue; Edwards, John E.

doi:10.1016/s1201-9712(02)90277-8

Cited by 1 publication

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“…We hypothesized that HL-60 cells reduced tissue fungal burden by phagocytizing the C. albicans. To confirm their capacity to phagocytize the fungus in vitro, we coincubated activated HL-60 cells labeled with the fluorescent dye Cell-Trace SNARF-1 (Molecular Probes, Eugene, OR) and C. albicans expressing GFP [40]. Confocal microscopy and differential interference contrast (DIC) optics confirmed phagocytosis of the fungus by HL-60 cells in vitro (Fig.…”

Section: Hl-60 Cells Chemotaxed To Sites Of Infection Phagocytized C Albicans In Tissue and Reduced Tissue Fungal Burden In Vivomentioning

confidence: 93%

A phagocytic cell line markedly improves survival of infected neutropenic mice

Spellberg

Collins

French

et al. 2005

Journal of Leukocyte Biology

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Disseminated candidiasis is a frequent infection in neutropenic patients, in whom it causes 50% mortality, despite antifungal therapy. As the duration of neutropenia is the strongest predictor of survival in neutropenic patients with invasive fungal infections, neutrophil transfusions are a logical, therapeutic option. However, significant technical barriers have prevented the clinical use of neutrophil transfusions. To overcome these barriers, we identified a human phagocytic cell line that could be administered to candidemic hosts in lieu of freshly harvested neutrophils. HL-60 cells killed Candida albicans in vitro. Activation of HL-60 cells with dimethyl sulfoxide and retinoic acid abrogated the cells' proliferation and augmented their killing of C. albicans. Administration of activated HL-60 cells to candidemic, neutropenic mice significantly improved survival (53% vs. 0%). Live HL-60 cells chemotaxed to sites of infection, phagocytized C. albicans, and reduced the fungal burden in key target organs. Although unactivated HL-60 cells also reduced tissue fungal burden in vivo, they did not improve survival as a result of their toxicity in infected mice. In contrast, no toxicity as a result of activated HL-60 cells was observed at up to 2 months of follow-up. To our knowledge, this is the first description of a cell line-based immunotherapy for an infectious disease. With further refinements, activated HL-60 cells have the potential to overcome the technical barriers to neutrophil transfusions.

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Section: Hl-60 Cells Chemotaxed To Sites Of Infection Phagocytized C Albicans In Tissue and Reduced Tissue Fungal Burden In Vivomentioning

confidence: 93%