Expression analysis of anthocyanin biosynthesis key regulatory genes involved in pomegranate (Punica granatum L.)

Rouholamin, Sepideh; Zahedi, Bahman; Nazarian-Firouzabadi, Farhad; Saei, Ali

doi:10.1016/j.scienta.2015.02.017

Cited by 41 publications

(28 citation statements)

References 33 publications

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“…Guo et al (2015) suggest that IbCHI is a key enzyme in the in the anthocyanin biosynthesis of sweet potato. Rouholamin et al (2015) found that total anthocyanin content measurement showed a positive correlation between the level of DFR gene expression and accumulation of anthocyanins in different genotypes in Punica granatum. Shi et al (2015b) reported that the transcript level of MsANS of Magnolia sprengeri was 26-fold higher in red petals than in white petals.…”

Section: Discussionmentioning

confidence: 89%

“…The accumulation of anthocyanin in plants is closely related to the expression level of genes associated with anthocyanin biosynthesis . There are two types of genes that control anthocyanin biosynthesis in plants: one is the structural gene that encodes the anthocyanin biosynthetic pathway enzymes, and the other is a regulatory gene that regulates spatiotemporal expression of the structural gene (Rouholamin et al, 2015;Zhao et al, 2015). The chalcone synthase gene (CHS) plays an essential role in anthocyanin biosynthesis and regulates the relative amount of specific flavonoids .…”

Section: Introductionmentioning

confidence: 99%

“…Dihydroflavonol 4-reductase (DFR), only expressed in the colored organ responsible for anthocyanidin production, catalyzes flavanonols into leucoanthocyanidin (Xu et al, 2014). The total anthocyanin content has a positive correlation between the level of DFR gene expression and the accumulation of anthocyaninsin in Punica granatum (Rouholamin et al, 2015). Anthocyanidin synthase (ANS) catalyzes the conversion of colorless leucoanthocyanins into colored anthocyanins (Shi et al, 2015a).…”

Section: Introductionmentioning

confidence: 99%

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Red Pigment Content and Expression of Genes Related to Anthocyanin Biosynthesis in Radishes (Raphanus sativus L.) with Different Colored Flesh

Chen¹,

Chen²,

Huo³

et al. 2016

JAS

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Radish with red skin and red flesh (Raphanus sativus) is a unique vegetable containing large amounts of a red pigment, which is widely used in foods, wine, and cosmetics. To investigate the gene or genes that play a key role in anthocyanin biosynthesis in radish with red skin and red flesh, the red pigment content and expression of genes involved in anthocyanin biosynthesis of 16 varieties with different colored flesh were studied. The expression level of the genes RsPAL, RsCHS, RsCHI, RsDFR, RsF3H, RsF3'H, and RsANS in radish with red skin and red flesh are all significantly higher than that of radish with white skin and white flesh, radish with red skin and white flesh, radish with green skin and pinky flesh, and radish with red skin and pinky flesh. Correlation analysis indicated that the gene expression level of RsDFR, RsF3H, RsCHS, RsANS, RsF3'H, RsCHI, and RsPAL showed remarkable positive and significant correlation to red pigment content of radish. Stepwise regression analysis showed that the gene expression level of RsDFR had the highest and significant direct effect (0.8932) on red pigment content of radish. The results indicated that (1) the red pigment content of radish is closely related to the increased expression of a number of structural genes in anthocyanin biosynthesis, (2) the RsDFR gene plays a key role in anthocyanin biosynthesis in red radish with red flesh, and (3) RsDFR might be one of the best targets in genetic engineering for anthocyanin production from radish and other plants.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Red Pigment Content and Expression of Genes Related to Anthocyanin Biosynthesis in Radishes (Raphanus sativus L.) with Different Colored Flesh

Chen¹,

Chen²,

Huo³

et al. 2016

JAS

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“…Later, other proteins related to ROS and secondary metabolism were reported [19][20][21][22][23][24]. Thus far, although the new throughput approaches (mainly iTRAQ and RNA-seq) are contributing to increasing knowledge on the background of pomegranate metabolism [30][31][32][33][34][35], more research is necessary since these technologies provide data on the gene transcript levels and their corresponding translational products, respectively. However, post-translational events indicate that there is no direct correspondence between transcriptomic and proteomic data and the activity displayed by proteins.…”

Section: Discussionmentioning

confidence: 99%

Pomegranate (Punica granatum L.) Fruits: Characterization of the Main Enzymatic Antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System

et al. 2019

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Pomegranate (Punica granatum L.) is a common edible fruit. Its juice can be used as a source of antioxidative compounds, primarily polyphenols and vitamin C, in addition to other vitamins and minerals. Nevertheless, little is still known about how the enzymatic machinery, mainly that related to oxidative metabolism, is influenced by the genotype and the environmental and climate conditions where pomegranate plants grow. In this work, seeds and juices from two pomegranate varieties (Valenciana and Mollar) grown in two different Spanish locations were assayed. Both varieties showed clear differences in their respective polypeptide profiles. The analysis of the isoenzymatic superoxide dismutase (SOD) activity pattern displayed one Mn-SOD and five CuZn-SODs (I–V) whose abundances depended on the variety. Furthermore, by immunoblot assays, at least one additional Fe-SOD with a subunit size of about 23 kDa was also detected in both varieties. Besides this, the presence of the H2O2-scavenging peroxisomal catalase in seeds and juice indicates that an active metabolism of reactive oxygen species (ROS) takes place in this fruit, but the two pomegranate varieties showed opposite activity profiles. The activities of the main NADPH-regenerating enzymes, including glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphlogluconate dehydrogenase (6PGDH), NADP-dependent isocitrate dehydrogenase (NADP-ICDH), and NADP-dependent malic enzyme (NADP-ME), were studied in the same plant materials, and they behaved differently depending on the genotype. Finally, our data demonstrate the presence of two specific enzymes of the peroxisomal glyoxylate cycle, malate synthase (MS) and isocitrate lyase (ICL). These enzymes participate in oilseeds by channeling the lipid catabolism to the carbohydrate synthesis for further use in seed germination and early seedling growth. The results obtained in this work indicate that a similar mechanism to that reported in oilseeds may also operate in pomegranate.

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“…They also play an important role in the commercial and aesthetic value of the fruit. The anthocyanin biosynthesis pathway and the corresponding key enzymes [including chalcone synthase, flavanone-3hydroxylase, anthocyanidin synthase (ANS), dihydroflavonol-4-reductase (DFR), and UDPglucose:flavonoid-3-O-glucosyltransferase (UFGT)] have been investigated extensively in many higher plants, including fruit trees such as grapevine (Sparvoli et al, 1994;Jeong et al, 2004;Ageorges et al, 2006;Figueiredo-González et al, 2012), apple Feng et al, 2013;Meng et al, 2015), pomegranate (Rouholamin et al, 2015), peach (Hassani et al, 2015;Liu et al, 2015), strawberry , kiwifruit (Li et al, 2016), blueberry (Zifkin et al, 2012;Li et al, 2015), blackberry (Chen et al, 2012), pear (Fischer et al, 2007;Pierantoni et al, 2010), nectarines (Ravaglia et al, 2013), and mangosteen (Palapol et al, 2009). Although previous studies have demonstrated the role of these key enzymes in the flavonoid pathway, there is a lack of knowledge regarding the regulation mechanism of these enzymes.…”

Section: Introductionmentioning

confidence: 99%

MYBA2 gene involved in anthocyanin and flavonol biosynthesis pathways in grapevine

Niu¹,

Gao²,

Zhang³

et al. 2016

Genet. Mol. Res.

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MYBA2 transcription factor (Myb-related gene) affects the coloring in grapevine berry and plays an important role in the biosynthesis of anthocyanin. The MYBA2 gene was cloned from Vitis vinifera L. cv. Cabernet Sauvignon and polyclonal antibodies for VvmybA2 were prepared. The VvmybA2 gene expression patterns were observed in seven tissues (the leaf, stem, flower, bud, root, berry, and tendril) and during the berry development stage at transcriptional and translational levels, respectively. The results indicated that the expression of VvmybA2 was approximately 11-fold higher in the berry than that in the other six tissues, and increased rapidly from 60 days after full bloom reaching a maximum on day 80. Furthermore, both the anthocyanin content and UDP-glucose:flavonoid-3-O-glucosyltransferase (UFGT) gene expression levels increased rapidly 60 days after full bloom. Moreover, correlation analysis indicated that the transcriptional and translational expression levels of the VvmybA2 gene were significantly positively correlated with not only UFGT and DFR genes but also with the anthocyanin content during berry development. These results suggested that VvmybA2 could not only regulate the transcription of both UFGT and DFR but also is involved in the expression of the UFGT gene associated with color determination in grape berries.

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Expression analysis of anthocyanin biosynthesis key regulatory genes involved in pomegranate (Punica granatum L.)

Cited by 41 publications

References 33 publications

Red Pigment Content and Expression of Genes Related to Anthocyanin Biosynthesis in Radishes (Raphanus sativus L.) with Different Colored Flesh

Red Pigment Content and Expression of Genes Related to Anthocyanin Biosynthesis in Radishes (Raphanus sativus L.) with Different Colored Flesh

Pomegranate (Punica granatum L.) Fruits: Characterization of the Main Enzymatic Antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System

MYBA2 gene involved in anthocyanin and flavonol biosynthesis pathways in grapevine

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