Expression analysis of genes encoding translation initiation factor 3 subunit g (TaeIF3g) and vesicle-associated membrane protein-associated protein (TaVAP) in drought tolerant and susceptible cultivars of wheat

Singh, Gurpreet; Jain, Mukesh; Kulshreshtha, Ritu; Khurana, Jitendra P.; Kumar, Sanjay; Singh, Prabhjeet

doi:10.1016/j.plantsci.2007.09.004

Cited by 15 publications

(14 citation statements)

References 56 publications

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“…The overexpression of eIF3g in wheat appears to enhance tolerance to drought and other abiotic stresses (Singh et al, 2007;Singh et al, 2013). Interestingly, the only monoclonal antibody to wheat eIF3 subunits that showed any inhibitory activity in vitro was to eIF3g and it inhibited mRNA binding to 40S ribosomes in vitro (Lauer et al, 1985;Heufler et al, 1988).…”

Section: Eif3 and Initiation/reinitiationmentioning

confidence: 99%

Mechanism of Cytoplasmic mRNA Translation

Browning

Bailey‐Serres

2015

The Arabidopsis Book

190

220

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Protein synthesis is a fundamental process in gene expression that depends upon the abundance and accessibility of the mRNA transcript as well as the activity of many protein and RNA-protein complexes. Here we focus on the intricate mechanics of mRNA translation in the cytoplasm of higher plants. This chapter includes an inventory of the plant translational apparatus and a detailed review of the translational processes of initiation, elongation, and termination. The majority of mechanistic studies of cytoplasmic translation have been carried out in yeast and mammalian systems. The factors and mechanisms of translation are for the most part conserved across eukaryotes; however, some distinctions are known to exist in plants. A comprehensive understanding of the complex translational apparatus and its regulation in plants is warranted, as the modulation of protein production is critical to development, environmental plasticity and biomass yield in diverse ecosystems and agricultural settings.

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Section: Eif3 and Initiation/reinitiationmentioning

confidence: 99%

Mechanism of Cytoplasmic mRNA Translation

Browning

Bailey‐Serres

2015

The Arabidopsis Book

190

220

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“…These findings led us to speculate that there is a direct structural interaction between PRSV NIa-Pro and CpeIF3G that could be irrelevant to NIa-Pro-mediated proteolysis, but this speculation remains to be confirmed because protein binding can occur in the absence of proteolysis. Furthermore, the other results from the SRS assay using CpeIF3G deletion mutants demonstrated that the necessary fragment for interaction with PRSV NIa-Pro was the central domain (residues 59-167), including a C2HC-type zinc finger motif [41]. Previous studies have shown that the Cauliflower mosaic virus (CaMV) transactivator, TAV, controls translation reinitaition for virus RNA translation via Fig.…”

Section: Discussionmentioning

confidence: 99%

“…For example, plant eIF3G, which can be induced under various environmental stress conditions, is positively involved in the stress response by enhancing the translation of resistance-related proteins [31,41]. However, few have investigated the expression patterns of eIF3G during plant pathogen attack.…”

Section: Discussionmentioning

confidence: 99%

NIa-Pro of Papaya ringspot virus interacts with Carica papaya eukaryotic translation initiation factor 3 subunit G (CpeIF3G)

et al. 2014

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The interaction of papaya eukaryotic translation initiation factor 3 subunit G (CpeIF3G) with Papaya ringspot virus (PRSV) NIa-Pro was validated using a bimolecular fluorescence complementation assay in papaya protoplasts based on the previous yeast two-hybrid assay results. The C-terminal (residues 133-239) fragment of PRSV NIa-Pro and the central domain (residues 59-167) of CpeIF3G were required for effective interaction between NIa-Pro and CpeIF3G as shown by a Sos recruitment yeast two-hybrid system with several deletion mutants of NIa-Pro and CpeIF3G. The central domain of CpeIF3G, which contains a C2HC-type zinc finger motif, is required to bind to other eIFs of the translational machinery. In addition, quantitative real-time reverse transcription PCR assay confirmed that PRSV infection leads to a 2- to 4.5-fold up-regulation of CpeIF3G mRNA in papaya. Plant eIF3G is involved in various stress response by enhancing the translation of resistance-related proteins. It is proposed that the NIa-Pro-CpeIF3G interaction may impair translation preinitiation complex assembly of defense proteins and interfere with host defense.

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“…Some potential uses include the ability to search for clones di- LEA proteins HVA1 Higher root and shoot biomass than controls under drought stress and improved recovery after drought [84] Lipid transfer protein TaLTP1 Increasing by water stresses, such as by treatment at several PEG concentrations and NaCl, by hormone treatments [85] Membrane protein associated-protein TaVAP Increasing in response to mild drought stress in the flag leaf [86] rectly or indirectly that are related to major gene differences, mutations, QTLs and for genes showing changes in gene expression during a developmental time-course or in a tissue basis [74,[77][78][79][80]. In grain crops, timecourse studies of seed development, gene expression during meiosis, and responses to specific environmental stimuli will identify expressed genes [13].…”

Section: Functional Genomicsmentioning

confidence: 99%

Prospectives for applying molecular and genetic methodology to improve wheat cultivars in drought environments

Zhao

Guo

Jaleel

et al. 2008

Comptes Rendus. Biologies

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With the advent of molecular biotechnologies, new opportunities are available for plant physiologists to study the relationships between wheat traits and their genetic control. The functional determinations of all genes that participate in drought adaptation or tolerance reactions are expected to provide an integrated understanding of the biochemical and physiological basis of stress responses in wheat. However, despite all the recent technological breakthroughs, the overall contribution of genomics-assisted breeding to the release of drought-resilient wheat cultivars has so far been marginal. This paper critically analyses how biotechnological, genetic and information tools can contribute to accelerating the release of improved, drought-tolerant wheat cultivars. Armed with such information from established models, it will be possible to elucidate the physiological basis of drought tolerance and to select genotypes with an improved yield under water-limited conditions. To cite this article: C.-X. Zhao et al., C. R. Biologies 331 (2008).

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Expression analysis of genes encoding translation initiation factor 3 subunit g (TaeIF3g) and vesicle-associated membrane protein-associated protein (TaVAP) in drought tolerant and susceptible cultivars of wheat

Cited by 15 publications

References 56 publications

Mechanism of Cytoplasmic mRNA Translation

Mechanism of Cytoplasmic mRNA Translation

NIa-Pro of Papaya ringspot virus interacts with Carica papaya eukaryotic translation initiation factor 3 subunit G (CpeIF3G)

Prospectives for applying molecular and genetic methodology to improve wheat cultivars in drought environments

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