Expression Analysis of Genes Related to Rice Resistance Against Brown Planthopper, Nilaparvata lugens

Jannoey, Panatda; Channei, Duangdao; Kotcharerk, Jate; Pongprasert, Weerathep; Nomura, Masatoshi

doi:10.1016/j.rsci.2016.10.001

Cited by 38 publications

(28 citation statements)

References 45 publications

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“…For instance, oligogalacturonides, oligomers of alpha-1,4-linked galacturonosyl residues, are one of the well-studied DAMPs that are released from plant cell walls upon degradation by insect feeding and can elicit plant defense responses [59]. OsLecRK s activation then might induce the downstream OsMPK s via production of intermediate factors or signal molecules [60,61]. The activated MAPK cascade might then, in turn, activate the phytohormonal defense’s regulation and other downstream transcriptional reprogramming to confer resistance against BPH.…”

Section: Discussionmentioning

confidence: 99%

“…SA induces the deposition of callose in phloem cells and the production of a trypsin inhibitor to discourage BPH feeding, whereas ET results in the production of a green volatile that deters BPH feeding [1,46]. The activation of a secondary metabolite biosynthesis pathway, such as phenylpropanoids, results in the production of important compounds, such as lignin and phytoalexins, which help to mitigate BPH pressure [61]. However, when IR56 rice was infested with IR56-BPH, none of the OsLecRK were found to be induced, suggesting that IR56-BPH can suppress the PRR-mediated immune response in rice, possibly by the use of other explicit effectors.…”

Section: Discussionmentioning

confidence: 99%

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Differential Responses of OsMPKs in IR56 Rice to Two BPH Populations of Different Virulence Levels

Nanda

Wan

Yuan

et al. 2018

IJMS

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The conserved mitogen-activated protein kinase (MAPK) cascades play vital roles in plant defense responses against pathogens and insects. In the current study, the expression profiles of 17 OsMPKs were determined in the TN1 and IR56 rice varieties under the infestation of brown planthopper (BPH), one of the most destructive hemimetabolous rice pests. The virulent IR56 BPH population (IR56-BPH) and the avirulent TN1 BPH population (TN-BPH) were used to reveal the roles of OsMPKs in the compatible (IR56-BPH infested on the TN1 and IR56 rice varieties, and TN1-BPH infested on the TN1 rice variety) and the incompatible (TN1-BPH infested on the IR56 rice variety) interaction. The statistical analysis revealed that rice variety, BPH population type, and infestation period have significant effects on the transcription of OsMPKs. Out of these genes, five OsMPKs (OsMPK1, OsMPK3, OsMPK7, OsMPK14, and OsMPK16) were found to exhibit upregulated expression only during incompatible interaction. Six OsMPKs (OsMPK4, OsMPK5, OsMPK8, OsMPK9, OsMPK12, and OsMPK13) were associated with both incompatible and compatible interactions. The transcription analysis of salicylic acid, jasmonic acid, and ethylene phytohormone signaling genes revealed their roles during the rice–BPH interactions. The upregulated expression of OsC4H, OsCHS, and OsCHI in the incompatible interaction implied the potential defense regulatory roles of phenylpropanoids. In both varieties, the elevated transcript accumulations of OsGST and OsSOD, and the increased enzyme activities of POD, SOD, and GST at 1 day post-infestation (dpi), but not at 3 dpi, indicated that reactive oxygen species (ROS) signaling might be an early event in rice–BPH interactions. Furthermore, upregulated transcription of OsLecRK3 and OsLecRK4 was found only during an incompatible interaction, suggesting their involvement in the BPH resistance response in the IR56 rice variety. Lastly, based on the findings of this study, we have proposed a model of interactions of IR56 rice with TN1-BPH and IR56-BPH that depicts the resistance and susceptibility reactions, respectively.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Differential Responses of OsMPKs in IR56 Rice to Two BPH Populations of Different Virulence Levels

Nanda

Wan

Yuan

et al. 2018

IJMS

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“…Lignin accumulation plays an important role in the process of plant resistance to insect pests [ 77 , 78 ]. Rice PAL , C4H and pathogenesis-related 9 ( PR9 ) genes that associated with lignin biosynthesis were significantly up-regulated in brown plant hopper-infested insect-resistant rice varieties, suggesting that these may synergistically participate in lignin biosynthesis which regulate the insect resistance of rice [ 79 , 80 ]. Wang et al [ 81 ] found that an aphid penetration-induced transcription factor CmMYB19 in chrysanthemum enhanced the expression of lignin biosynthesis genes and lignin accumulation, which limited the invasion of aphids and increased chrysanthemum aphid tolerance.…”

Section: Relationship Between Lignin Biosynthesis and Plant Stressmentioning

confidence: 99%

Lignins: Biosynthesis and Biological Functions in Plants

Liu

Luo

Zheng

2018

IJMS

923

530

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Lignin is one of the main components of plant cell wall and it is a natural phenolic polymer with high molecular weight, complex composition and structure. Lignin biosynthesis extensively contributes to plant growth, tissue/organ development, lodging resistance and the responses to a variety of biotic and abiotic stresses. In the present review, we systematically introduce the biosynthesis of lignin and its regulation by genetic modification and summarize the main biological functions of lignin in plants and their applications. We hope this review will give an in-depth understanding of the important roles of lignin biosynthesis in various plants’ biological processes and provide a theoretical basis for the genetic improvement of lignin content and composition in energy plants and crops.

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“…Rice responds to WBPH through the activation of oxidative and phenylpropanoid pathway enzymes, such as chorismate mutase ( CM ), phenylalanine ammonia lyase ( PAL ), chalcone synthase ( CHS ), superoxide dismutase ( SOD ), and catalase ( CAT ) [ 15 ], which enhance lignification in cell walls and increase toughness of the cell wall. Brown planthopper (BPH) infestation stimulates antioxidant-related genes, such as SOD and CAT , in rice [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Over-Expression of Chorismate Mutase Enhances the Accumulation of Salicylic Acid, Lignin, and Antioxidants in Response to the White-Backed Planthopper in Rice Plants

et al. 2021

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The white-backed planthopper (WBPH) is a serious pest of rice crop and causes sever yield loss each year, especially in Asian countries. In this study, we used chorismate mutase (CM) transgenic line to examine the defense mechanism of rice plants against WBPH. The survival rate of WBPHs, infestation rate of plants, lignin biosynthesis, transcriptional regulation of related genes, salicylic acid (SA) accumulation and signaling and antioxidants regulation were investigated. The WBPH population decreased by 67% in OxCM-t, and the plant infestation rate was 3.5-fold higher in wild-type plants compared with transgenic plants. A substantial increase in lignin was found in the transgenic line (742%) and wild-type (417%) plants. Additionally, CM, phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), and chalcone isomerase (CHI) showed significant increases in their relative expression level in the transgenic line. Salicylic acid was significantly enhanced in the transgenic line compared with WBPH infestation. SA can activate pathogenesis related proteins-1 (PR1), PR2, antioxidants, and the expression of their related genes: superoxide dismutase (SOD) and catalase (CAT). WBPH infestation reduced the chlorophyll contents of both transgenic and wild-type plants, but the reduction was great in wild-type than transgenic plants. The sugar content was only significantly increased in the transgenic line, indicating that sugars are not heavily involved in WBPH stress. Phenylalanine, proline, aspartic acid, and total amino acids were increased in the transgenic line and reduced in the wild-type plants. Taken together, all the results suggest that overexpression of CM gene regulates the defense mechanisms and enhances the rice toward WBPH stress.

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Expression Analysis of Genes Related to Rice Resistance Against Brown Planthopper, Nilaparvata lugens

Cited by 38 publications

References 45 publications

Differential Responses of OsMPKs in IR56 Rice to Two BPH Populations of Different Virulence Levels

Differential Responses of OsMPKs in IR56 Rice to Two BPH Populations of Different Virulence Levels

Lignins: Biosynthesis and Biological Functions in Plants

Over-Expression of Chorismate Mutase Enhances the Accumulation of Salicylic Acid, Lignin, and Antioxidants in Response to the White-Backed Planthopper in Rice Plants

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