Expression Analysis of Human Salivary Glands by Laser Microdissection: Differences Between Submandibular and Labial Glands

Kouznetsova, Irina; Gerlach, Klaus; Zahl, Christian; Hoffmann, Werner

doi:10.1159/000320561

Cited by 34 publications

(43 citation statements)

References 49 publications

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“…Labial gland secretions from 90 subjects were nearly all reactive to anti-MUC5B antibody, whereas secretions from buccal mucous glands (89) were reactive in only 11 subjects (16). MUC19 transcripts were detected in human submandibular and labial glands (17)(18)(19), and MUC19 glycoproteins immunolocalized to salivary gland mucous cells (19). We now confirm expression of MUC19 transcripts in submandibular glands from multiple human subjects as well as immunolocalization of MUC19 to mucous elements of major and minor salivary glands.…”

Section: Muc19 Protects Against S Mutans Colonization and Developmensupporting

confidence: 62%

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Salivary Mucin 19 Glycoproteins

Culp¹,

Robinson²,

Cash³

et al. 2015

Journal of Biological Chemistry

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Background:We investigated murine salivary Muc19, in vivo and in vitro, to protect against dental infections. Results: Muc19 attenuates dental infections by Streptococcus mutans and promotes its aggregation. Evidence for human salivary MUC19 is presented. Conclusion: Murine Muc19 helps limit colonization via clearance of S. mutans. Significance: A role for Muc19 in clearance expands upon acknowledged innate immune functions of salivary gel-forming mucins.

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Section: Muc19 Protects Against S Mutans Colonization and Developmensupporting

confidence: 62%

“…In humans, MUC5B is well documented as a gel-forming mucin in saliva (16), However, MUC19 transcripts were detected in human submandibular and labial glands (17)(18)(19) and glycoproteins localized to salivary mucous cells (19). Conversely, MUC19 was undetected in human saliva in a recent proteomic study (20).…”

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confidence: 99%

Salivary Mucin 19 Glycoproteins

Culp¹,

Robinson²,

Cash³

et al. 2015

Journal of Biological Chemistry

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“…In the pig, in contrast to human, TFF2 is also abundantly secreted from exocrine pancreatic glands (acinar cells) (14). Furthermore, TFF2 is expressed in salivary glands (21,23).…”

Section: Tff2 a Muc6-binding Lectin Stabilizing The Gastric Mucus Bamentioning

confidence: 99%

TFF2, a MUC6-binding lectin stabilizing the gastric mucus barrier and more (Review)

Hoffmann

2015

International Journal of Oncology

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Abstract. The peptide TFF2 (formerly 'spasmolytic polypeptide'), a member of the trefoil factor family (TFF) containing two TFF domains, is mainly expressed together with the mucin MUC6 in the gastric epithelium and duodenal Brunner's glands. Pathologically, TFF2 expression is observed ectopically during stone diseases, chronic inflammatory conditions and in several metaplastic and neoplastic epithelia; most prominent being the 'spasmolytic polypeptide-expressing metaplasia' (SPEM), which is an established gastric precancerous lesion. TFF2 plays a critical role in maintaining gastric mucosal integrity and appears to restrain tumorigenesis in the stomach. Recently, porcine TFF2 has been shown to interact with the gastric mucin MUC6 and thus stabilize the gastric mucus barrier. On the one hand, TFF2 binds to MUC6 via non-covalent lectin interactions with the glycotope GlcNAcα1→4Galβ1→R. On the other hand, TFF2 is probably also covalently bound to MUC6 via disulfide bridges. Thus, implications for the complex multimeric assembly, cross-linking, and packaging of MUC6 as well as the rheology of gastric mucus are discussed in detail in this review. Furthermore, TFF2 is also expressed in minor amounts in the immune and nervous systems. Thus, similar to galectins, its lectin activity would perfectly enable TFF2 to form multivalent complexes and cross-linked lattices with a plethora of transmembrane glycoproteins and thus modulate different signal transduction processes. This could explain the multiple and diverse biological effects of TFF2 [e.g., motogenic, (anti)apoptotic, and angiogenic effects]. Finally, a function during fertilization is also possible for TFF domains because they occur as shuffled modules in certain zona pellucida proteins.

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“…These observations suggest a distinct TFF secretory pattern in rats compared to humans. In humans, TFF1, TFF2 and TFF3 are present in the mucous acini of SMGs; TFF3 is located also in the serous cells of the SMGs (Kouznetsova et al 2010). TFFs typically are secreted by acinar cells in combination with other bioactive factors; however, they may be secreted separately in rats by different types of ducts prior to their binding to the mucin derived from the acinar cells to form mucous gels.…”

Section: Discussionmentioning

confidence: 99%

Expression and localization of trefoil factor family genes in rat submandibular glands

Zhang

Xue

et al. 2014

Biotechnic & Histochemistry

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The trefoil factor (TFF) family, which comprises TFF1, TFF2 and TFF3, plays an essential role in epithelial regeneration within the gastrointestinal tract. All three TFFs are present in human saliva; TFF3 is the predominant trefoil peptide. Little is known about the expression and tissue distribution of TFFs in rats, which are commonly used as a model system for human studies. We investigated the localization of the TFF genes that encode secretory peptides in rat submandibular glands (SMG). All three TFFs were expressed in rat SMG, although their location varied. Substantial amounts of TFF1 were detected only in the cytoplasm of epithelial cells in the SMG granular convoluted tubules (GCT), while TFF2 and TFF3 were widely distributed in the cytoplasm of epithelial cells of intercalated ducts (ID), striated ducts (SD) and interlobular ducts (ILD). The three TFFs also were detected especially in the lumens of the SD and ILD. Semi-quantitative RT-PCR and in situ hybridization experiments confirmed TFF1, TFF2 and TFF3 mRNA expressions in the SMG. Greater expression of TFF peptides and mRNA was observed in male rats than in females. The broad expression of TFFs in rat SMG cells and lumens suggests that TFFs function in this organ by their secretion into the duct lumens. We also found differences in TFF expression profiles between rat and human SMG; therefore, caution should be exercised when using rats as a model for human TFF studies.

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Expression Analysis of Human Salivary Glands by Laser Microdissection: Differences Between Submandibular and Labial Glands

Cited by 34 publications

References 49 publications

Salivary Mucin 19 Glycoproteins

Salivary Mucin 19 Glycoproteins

TFF2, a MUC6-binding lectin stabilizing the gastric mucus barrier and more (Review)

Expression and localization of trefoil factor family genes in rat submandibular glands

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