Expression Analysis of MALAT1, GAS5, SRA, and NEAT1 lncRNAs in Breast Cancer Tissues from Young Women and Women over 45 Years of Age

Arshi, Asghar; Sharifi, Fatemeh; Ghahfarokhi, Milad Khorramian; Faghih, Zahra; Doosti, Abbas; Ostovari, Sara; Maymand, Elham Mahmoudi; Seno, Mohammad Mahdi Ghahramani

doi:10.1016/j.omtn.2018.07.014

Cited by 66 publications

(57 citation statements)

References 36 publications

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“…Along with miR-204, the levels of NEAT1 differed between HER2-positive status and the other respective status. A significant upregulation of NEAT1 was observed in tumor tissues of BC patients by several previous studies (Arshi et al, 2018;Choudhry et al, 2015;Li et al, 2017b;Zhang et al, 2017b;Zhao et al, 2017). Similar to our findings, Zhang et al (2017b) observed that high expression of NEAT1 in BC tissues was closely related to lymph node metastasis and stimulated cell proliferation.…”

Section: Discussionsupporting

confidence: 91%

Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer

et al. 2019

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Long noncoding RNAs (lncRNAs) are frequently precursor RNAs of microRNAs (miRNAs) or act as competing endogenous RNAs (ceRNAs) to interact with miRNAs. To better understand the shared impact of lncRNAs and miRNAs in the regulatory post‐transcriptional network, we focused here on the relationships between (a) lncRNA H19 and miR‐675, (b) NEAT1 and miR‐204, and (c) HOTAIR and miR‐331 in plasma of early breast cancer (BC) patients. We quantified each RNA in plasma samples of 63 BC patients and 10 healthy women by quantitative real‐time PCR. In cell culture experiments, the influence of these noncoding RNAs (ncRNAs) on proliferation and apoptosis of BC cell line MCF‐7 was examined. Plasma levels of H19 ( P = 0.030), NEAT1 ( P = 0.030), and miR‐331 ( P = 0.012) were deregulated in BC patients compared with healthy women. In both cohorts, the concentrations of H19 correlated with those of miR‐675 ( P = 0.0001). Higher H19 ( P = 0.001) along with lower miR‐675 ( P = 0.007) levels and higher miR‐204 ( P = 0.017) along with lower NEAT1 ( P = 0.030) levels were detected in plasma of HER2‐positive patients compared with the other BC subgroups. Whereas the expression of HOTAIR was below the detection level, miR‐331 levels correlated with nodal status ( P = 0.002) and recurrence ( P = 0.012). In cell culture experiments, a competitive impact on cell proliferation and apoptosis by these ncRNAs was also documented. Our findings describe a relationship of the plasma levels of H19/miR‐675 and NEAT1/miR‐204 in the different BC subtypes; in addition, they reveal an interplay between these lncRNAs and miRNAs in the regulatory network in MCF‐7 cells, which should also be considered in the search for new diagnostic and therapeutic markers.

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Section: Discussionsupporting

confidence: 91%

Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer

et al. 2019

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“…54 GAS5 levels were significantly decreased in BC tissues than those in adjacent normal breast epithelial tissues. 18,55,56 One study showed low GAS5 expression in triple-negative breast cancer (TNBC) tissues than that of adjacent non-tumor tissues, and was associated with clinical stage, lymph node metastasis (LNM) and OS. 19 Decreased expression of GAS5 was found in BC tissues and was negatively associated with later TNM stage and shorter OS time as well.…”

Section: Clinical Relevancementioning

confidence: 99%

“…45 Intriguingly, BC patients aged less than 45 years old who had much lower GAS5 levels than those of old cases showed the poorer prognosis. 56 Others found that GAS5 levels were decreased frequently in estrogen receptor-negative (ER-) BC tissues and cells, and were related with large tumor size, advanced TNM stage. 57 BC patients with low GAS5 levels in plasma and a high Ki67 proliferation index before surgery were positively associated with LNM after surgery, indicating that plasma GAS5 may have the potential to assess the surgical effects and prognosis for patients with BC.…”

Section: Clinical Relevancementioning

confidence: 99%

<p>Novel Tumor Suppressor lncRNA Growth Arrest-Specific 5 (GAS5) In Human Cancer</p>

Yu¹,

Hann²

2019

OTT

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Long noncoding RNAs (lncRNAs) play crucial regulatory roles in fundamental biological processes, and deregulations of lncRNAs have been linked to numerous human diseases, especially cancers. Of particular interest in this regard is lncRNA GAS5, which is mainly identified as a tumor suppressor in several cancers. GAS5 was significantly low expressed in multiple cancers and was associated with clinic-pathological characteristics and patient survival, indicating a novel potential diagnostic and prognostic biomarker, and a therapeutic target for cancer. Functionally, GAS5 is involved in cell proliferation, metastasis, invasion, apoptosis, epithelial-mesenchymal transition (EMT), and drug resistance, among others, via multiple molecular mechanisms, such as binding to DNA sequences, forming RNA-DNA triplex complex, triggering or suppressing the expression of genes, binding proteins to form chromatin-modifying complex, which activates or represses gene expression, and acting as miRNA sponge to suppress miRNA expression, leading to regulation of miRNA target genes. This review provides an overview of the current state of knowledge and role of GAS5 in clinical relevance, biological functions and molecular mechanisms underlying the dysregulation of expression and function of GAS5 in cancer. Finally, the potential prospective role as diagnostic and prognostic biomarker and therapeutic target in cancer is discussed.

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“…This highlights that the novel lncRNA RP11-48B3.4 may via interacting with some encoding genes by different mechanisms involved in pathogenesis of the IDC. There is evidence that lncRNAs through in uencing expression of other coding and non-coding genes play key roles in development of BC [27]. In this way, one suggested mechanism is competing endogenous RNA (ceRNA) function through which lncRNAs via sponging miRNAs regulate expression of mRNAs in regulatory molecular networks whose roles in cancer development, especially in BC, are emerging with clinical signi cance [28,29].…”

Section: Discussionmentioning

confidence: 99%

Clinical Values of two Novel Estrogen Receptor Signaling Targeted lncRNAs in Invasive ductal breast carcinoma

Ilbeigi

Farahani

Monjezi

et al. 2020

Preprint

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Invasive ductal carcinoma (IDC) is the most frequent type of breast cancer (BC) in women, with a high clinical burden due to its high invasive properties. Despite of new data regarding the molecular heterogeneity of invasive cancers is quickly emerging; far less is known about the molecular patterns among cases of IDC. An expanding body of evidence has demonstrated that dysregulation of long noncoding RNAs (lncRNAs) is involved in heterogeneity feature of the BC. In this study, we analyzed the expression levels of two novel lncRNAs LOC100288637 and RP11-48B3 in 51 IDC tissues in comparison with adjacent non-cancerous tissues (ANCTs). And finally, bioinformatic evaluation has been done.The qPCR results showed that LOC100288637 and RP11-48B3 were significantly overexpressed in tumor tissues compared to normal samples (P= 0.0085 and P= 0.0002, respectively). Also, the two lncRNAs were overexpressed in both MDA-MB-231 and MCF-7 BC cell lines, nevertheless, with a higher expression pattern in MDA-MB-231 than MCF7 cell line. Furthermore, LOC100288637 had an elevated expression level in HER-2 positive tumors compared to HER-2 negative tumors (P= 0.031). Interestingly, the lncRNA RP11-48B3.4 was upregulated in IDC subjects with age at menarche <14 compared to patients with age at menarche ≥14 (P= 0.041). It was observed in another result that lncRNA RP11-48B3.4 is significantly upregulated in tumors with a lower histological grade compared to tumor samples with higher grades (P= 0.047). And finally, using bioinformatic evaluation, we found a predicted interaction between RP11-48B3.4 and mRNA Zinc Finger and BTB Domain Containing 10 (ZBTB10).Altogether, our findings suggest that these lncRNAs with potential oncogenic roles involved in pathogenesis of IDC with clinical significance, and thus, they may serve as novel markers for diagnosis and treatment of IDC.

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Expression Analysis of MALAT1, GAS5, SRA, and NEAT1 lncRNAs in Breast Cancer Tissues from Young Women and Women over 45 Years of Age

Cited by 66 publications

References 36 publications

Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer

Interplay of lncRNA H19/miR‐675 and lncRNA NEAT1/miR‐204 in breast cancer

<p>Novel Tumor Suppressor lncRNA Growth Arrest-Specific 5 (GAS5) In Human Cancer</p>

Clinical Values of two Novel Estrogen Receptor Signaling Targeted lncRNAs in Invasive ductal breast carcinoma

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