Expression analysis of mammalian mitochondrial ribosomal protein genes

Cheong, Agnes; Lingutla, Ranjana; Mager, Jesse

doi:10.1016/j.gep.2020.119147

Cited by 50 publications

(40 citation statements)

References 37 publications

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“…Mrps35, as a subunit of mitochondrial 28S ribosomal protein, is usually located in mitochondria and participates in the synthesis of mitochondrial related proteins [16, 21]. Previous reports supported that Mrps35 was involved in fat metabolism [22], cell metabolism and mitochondrial activation [23], cancer occurrence [16] and Alzheimer's disease progression [24].…”

Section: Discussionmentioning

confidence: 99%

Proteomics reveals the potential mechanism of Mrps35 controlling Listeria monocytogenes intracellular proliferation in macrophages

Yuan

et al. 2021

Proteomics

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Macrophages are sentinels in the organism which can resist and destroy various bacteria through direct phagocytosis. Here, we reported that expression level of mitochondrial ribosomal protein S35 (Mrps35) continued to decrease over infection time after Listeria monocytogenes (L. monocytogenes) infected macrophages. Our results indicated that knockdown Mrps35 increased the load of L. monocytogenes in macrophages. This result supported that Mrps35 played the crucial roles in L. monocytogenes infection. Moreover, we performed the comprehensive proteomics to analyze the differentially expressed protein of wild type and Mrps35 Knockdown Raw264.7 cells by L. monocytogenes infection over 6 h. Based on the results of mass spectrometry, we presented a wide variety of hypotheses about the mechanism of Mrps35 controlling the L. monocytogenes intracellular proliferation. Among them, experiments confirmed that Mrps35 and 60S ribosomal protein L22‐like 1 (Rpl22l1) were a functional correlation or potentially a compensatory mechanism during L. monocytogenes infection. This study provided new insights into understanding that L. monocytogenes infection changed the basic synthesis or metabolism‐related proteins of host cells.

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Section: Discussionmentioning

confidence: 99%

Proteomics reveals the potential mechanism of Mrps35 controlling Listeria monocytogenes intracellular proliferation in macrophages

Yuan

et al. 2021

Proteomics

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“…Mitochondrial ribosomal protein (MRP) is a basic component of mitochondrial complex structure and functional integrity. More than 80 MRPs have been identified in mammals, and 79 MRP gene expression patterns were recorded in mice development and adult organization, which found that these genes always express consistent throughout the early embryos, there is almost no stage or organization specificity [10]. Huang et al [17] have compared the designated position of the MRP gene with candidate regions of human diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Amino acid sequences of mitoribosomal proteins and their biochemical properties vary significantly among different species, which makes it difficult for us to distinguish them easily [9]. As a protein encoding gene, MRPS17 encodes a protein (28s) belonging to the ribosomal protein S17P family, which was moderately conserved between human and prokaryotes [10]. MRPS17 has been reported to be involved in the pathogenesis of myxosarcoma and phosphoserine phosphatase deficiency.…”

Section: Ivyspring International Publishermentioning

confidence: 99%

MRPS17 promotes invasion and metastasis through PI3K/AKT signal pathway and could be potential prognostic marker for gastric cancer

Zhou

Ouyang

et al. 2021

J. Cancer

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In this study, the molecular mechanisms through which Mitochondrial Ribosomal Protein S17 (MRPS17) contributes to gastric cancer (GC) and its prognostic significance in GC have been explored. As a protein encoding gene, MRPS17 encodes a 28s proteins belonging the ribosomal protein S17P family. The specific roles and molecular mechanisms of MRPS17 in cancers remain ambiguous. It was revealed by analyzing data from TCGA and GEO that elevated expression of MRPS17 was significantly associated with invasion of GC and poor survival of GC patients. Then through univariate and multivariate Cox regression analyses it was demonstrated that MRPS17 an independent prognostic factor for GC patients (P<0.001). It was demonstrated by differentially expressed gene analysis and functional enrichment analysis that MPRS17 is related to PI3K/AKT pathway and Cell adhesion molecules (CAMs), while its function is mediated by collagen-containing extracellular matrix and receptor ligand/regulator activity. Then it was proven by in-vitro experiments that knocking down of MRPS17 gene in AGS and SGC7901 cells would significantly inhibit proliferation and invasion capability of these cells. Furthermore, it was revealed by cell immunofluorescence assay that as a ribosomalprotein, MRPS17 was mainly distributed in the cytoplasmic surface of cell membrane. Additionally, activation of PI3K/AKT pathway is responsible for malignant progression of glioma that was promoted by MRPS17. In conclusion, it was revealed in the present study that MRPS17 promoted invasion and metastasis of GC and potential molecular mechanisms through which it exerted its influences on GC were explored, suggesting its potential as a novel prognostic biomarker for GC.

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“…In addition to producing ATP, the mitochondria are also related to oxidative stress, since the mitochondrial respiratory chain increases the production of reactive oxygen species. The 28S ribosomal protein S2, mitochondrial (protein accession number: Q9Y399) is a necessary component to maintain the structural and essential function of the mitochondria [23], while the APP gene (protein accession number: H7C0V9) relates to age-associated mitochondrial dysfunction in APP/PS1 transgenic mice [24]. The EIF5A gene (protein accession number: I3L397), as well as polyamines regulates mitochondrial substrate oxidation through hypusination of EIF5A [25].…”

Section: Discussionmentioning

confidence: 99%

Discovery of Diagnostic and Therapeutic Targets For Vascular Injury Induced By Methylglyoxal Using Proteomics

Xie

Liu

Zhang

et al. 2021

Preprint

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Background: Methylglyoxal, a byproduct of diabetes or the consumption of a high-carbohydrate diet, is associated with vascular injury; however, its molecular mechanisms remain unclear. We aimed to systematically characterize molecular profiles and offer unique insights into new disease pathways, thereby contributing to understanding the mechanisms and pathogenesis of vascular injury-related cardiovascular diseases. Methods: Cell survival assays were performed to assess DNA damage; oxidative stress was confirmed by colorimetric assays and quantitative fluorescence, and cyclooxygenase-2 and the mitogen-activated protein kinase pathways were assessed using ELISA. Differentially expressed proteins were quantitated via TMT-based LC-MS/MS and bioinformatics analysis, and confirmed by parallel reaction monitoring. Results: Vascular injury was assessed through colorimetric assays, quantitative fluorescence, ELISA, and survival assays. Of the 4029 proteins identified, 368 were differentially expressed after methylglyoxal treatment, compared with the negative control; 31 were defined as biomarkers or therapeutic targets according to the Gene Ontology Program, Kyoto Encyclopedia of Genes and Genomes, and protein-protein interaction network analyses. Sixteen proteins were significantly (p<0.05) upregulated (>1.5-fold change) and 15 were dramatically downregulated (<0.667-fold change) and confirmed through parallel reaction monitoring.Conclusions: The 31 proteins identified as biomarkers or therapeutic targets may contribute to vascular dysfunction through DNA damage, oxidative stress, inflammation, autophagy, hypertension, endothelial dysfunction, vascular remodeling, and the coagulation cascade. Additionally, new disease pathways involving the Wnt, ErBb, and BMP signaling pathways were identified; all provide scope as potential underlying molecular mechanisms. Therefore, the 31 proteins identified warrant further development as new therapeutic or diagnostic targets for vascular diseases.

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Expression analysis of mammalian mitochondrial ribosomal protein genes

Cited by 50 publications

References 37 publications

Proteomics reveals the potential mechanism of Mrps35 controlling Listeria monocytogenes intracellular proliferation in macrophages

Proteomics reveals the potential mechanism of Mrps35 controlling Listeria monocytogenes intracellular proliferation in macrophages

MRPS17 promotes invasion and metastasis through PI3K/AKT signal pathway and could be potential prognostic marker for gastric cancer

Discovery of Diagnostic and Therapeutic Targets For Vascular Injury Induced By Methylglyoxal Using Proteomics

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