Expression analysis of MusaNAC68 transcription factor and its functional analysis by overexpression in transgenic banana plants

Negi, Sanjana; Tak, Himanshu; Ganapathi, T. R.

doi:10.1007/s11240-015-0929-6

Cited by 41 publications

(20 citation statements)

References 58 publications

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“…Bradi1g04150 SNAC1-like / NAC transcription factor 67 -NAC transcription factors mediate abiotic stress responses. Osmotic stress increases the expression of SNAC1 in Oryza sativa [43], NAC68 in Musa × paradisiaca [41,42] and NAC67 Triticum aestivum [44].…”

Section: S↗mentioning

confidence: 99%

“…Nine of these conserved cold response genes are involved in response to abiotic stresses in other plants, such as osmotic stress and drought. The SNAC1-like / NAC transcription factor 67 is one example, with homologs induced by osmotic stress in the three monocts Musa × paradisiaca [41,42], Oryza sativa [43] and Triticum aestivum [44]. Co-option of such genes into a cold-responsive pathway might have been the key to acquire cold tolerance.…”

Section: An Adaptive Potential In the Pooideae Ancestormentioning

confidence: 99%

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Comparative transcriptomics provides insight into the evolution of cold response in Pooideae

Grønvold

Schubert

Sandve

et al. 2017

Preprint

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Background: Understanding how complex traits evolve through adaptive changes in gene regulation remains a major challenge in evolutionary biology. Over the last ~50 million years, Earth has experienced climate cooling and ancestrally tropical plants have adapted to expanding temperate environments. The grass subfamily Pooideae dominates the grass flora of the temperate regions, but conserved cold-response genes that might have played a role in the cold adaptation to temperate climate remain unidentified.

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Section: S↗mentioning

confidence: 99%

Section: An Adaptive Potential In the Pooideae Ancestormentioning

confidence: 99%

Comparative transcriptomics provides insight into the evolution of cold response in Pooideae

Grønvold

Schubert

Sandve

et al. 2017

Preprint

View full text Add to dashboard Cite

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“…For salinity stress, 20 ml of 250 mM NaCl was irrigated to the plants. For drought treatment, plants were dried on a blotting paper in a laminar air flow hood (Negi et al 2015c). Total RNA isolated from leaves using Concert plant RNA reagent (Invitrogen, Waltham, MA, USA) was subsequently cleaned using RNeasy spin column supplied with the RNeasy plant mini kit (Qiagen, Hilden, Germany) following the manufacturer instructions.…”

Section: Stress Treatments and Expression Analysismentioning

confidence: 99%

“…Total chlorophyll content was expressed as microgram per milligram of fresh weight. Thiobarbituric-acid method was used for analyzing the MDA content as described earlier (Negi et al 2015c). Briefly, leaf tissue was homogenized and incubated at 95°C for 15 min with 0.5 % TBA in 20 % TCA.…”

Section: Confirmation Of Transgenic Linesmentioning

confidence: 99%

“…MaNAC5 in cooperation with WRKY transcription factor is involved in pathogen resistance and PRgenes regulation (Shan et al 2015). Another banana NAC transcription factor, MusaNAC68, is induced by multiple stress conditions and positively regulates drought and salinity tolerance in banana (Negi et al 2015c). However, exact role of NAC transcription factors in imparting tolerance to abiotic and biotic stresses needs to be elucidated in banana.…”

Section: Introductionmentioning

confidence: 99%

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Banana NAC transcription factor MusaNAC042 is positively associated with drought and salinity tolerance

2016

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Banana is an important fruit crop and its yield is hampered by multiple abiotic stress conditions encountered during its growth. The NAC (NAM, ATAF, and CUC) transcription factors are involved in plant response to biotic and abiotic stresses. In the present study, we studied the induction of banana NAC042 transcription factor in drought and high salinity conditions and its overexpression in transgenic banana to improve drought and salinity tolerance. MusaNAC042 expression was positively associated with stress conditions like salinity and drought and it encoded a nuclear localized protein. Transgenic lines of banana cultivar Rasthali overexpressing MusaNAC042 were generated by Agrobacterium-mediated transformation of banana embryogenic cells and T-DNA insertion was confirmed by PCR and Southern blot analysis. Our results using leaf disc assay indicated that transgenic banana lines were able to tolerate drought and high salinity stress better than the control plants and retained higher level of total chlorophyll and lower level of MDA content (malondialdehyde). Transgenic lines analyzed for salinity (250 mM NaCl) and drought (Soil gravimetric water content 0.15) tolerance showed higher proline content, better Fv/Fm ratio, and lower levels of MDA content than control suggesting that MusaNAC042 may be involved in responses to higher salinity and drought stresses in banana. Expression of several abiotic stress-related genes like those coding for CBF/DREB, LEA, and WRKY factors was altered in transgenic lines indicating that MusaNAC042 is an efficient modulator of abiotic stress response in banana.

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Complementation of Bananas Conventional Breeding Programs Through Biotechnological Genetic Improvement

López

Santos-Ordóñez

González

2020

Agricultural, Forestry and Bioindustry Biotechnology and Biodiscovery

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Expression analysis of MusaNAC68 transcription factor and its functional analysis by overexpression in transgenic banana plants

Cited by 41 publications

References 58 publications

Comparative transcriptomics provides insight into the evolution of cold response in Pooideae

Comparative transcriptomics provides insight into the evolution of cold response in Pooideae

Banana NAC transcription factor MusaNAC042 is positively associated with drought and salinity tolerance

Complementation of Bananas Conventional Breeding Programs Through Biotechnological Genetic Improvement

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