Expression Analysis of Pre-Harvest Sprouting Tolerant Korean Wheat via Transcriptomic Analysis

Park, Sang Yong; Park, Chul Hyun; Kim, Kyung Hoon; Jung, Woo Joo; Kim, Jae Yoon

doi:10.9787/kjbs.2022.54.2.104

Cited by 2 publications

(2 citation statements)

References 80 publications

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“…On the other hand, in Woori, only 6 of 206 seeds germinated, resulting in a germination rate of 2.91%, confirming that its PHS tolerance was significantly higher than that of Keumgang ( Figure 1 ). The results of this experiment corresponded to the results of various PHS experiments performed previously [ 57 , 58 , 59 ].…”

Section: Resultssupporting

confidence: 87%

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Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (Triticum aestivum)

Park

Jung²,

Bang³

et al. 2022

Plants

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While wheat (Triticum aestivum L.) is a widely grown and enjoyed crop, the diverse and complex global situation and climate are exacerbating the instability of its supply. In particular, pre-harvest sprouting (PHS) is one of the major abiotic stresses that frequently occurs due to irregular climate conditions, causing serious damage to wheat and its quality. In this study, transcriptomic analysis with RNA-seq and proteomic analysis with LC-MS/MS were performed in PHS-treated spikes from two wheat cultivars presenting PHS sensitivity and tolerance, respectively. A total of 13,154 differentially expressed genes (DEGs) and 706 differentially expressed proteins (DEPs) were identified in four comparison groups between the susceptible/tolerant cultivars. Gene function and correlation analysis were performed to determine the co-profiled genes and proteins affected by PHS treatment. In the functional annotation of each comparative group, similar functions were confirmed in each cultivar under PHS treatment; however, in Keumgang PHS+7 (K7) vs. Woori PHS+7 (W7), functional annotations presented clear differences in the ”spliceosome” and ”proteasome” pathways. In addition, our results indicate that alternative splicing and ubiquitin–proteasome support the regulation of germination and seed dormancy. This study provides an advanced understanding of the functions involved in transcription and translation related to PHS mechanisms, thus enabling specific proposals for the further analysis of germination and seed dormancy mechanisms and pathways in wheat.

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Section: Resultssupporting

confidence: 87%

“…Considering their results, we expected that our experiment would be highly reliable. Our modified sand bury method could be applied for PHS experiments, as well as artificial rainfall [ 58 ], the original sand bury method [ 63 ], and intended water loading [ 59 ], which are usually applied for PHS treatment.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (Triticum aestivum)

Park

Jung²,

Bang³

et al. 2022

Plants

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Transcriptome Analysis for Flooding Stress-related Gene Identification inGlycine soja

Kim

Park

Kim

2022

Korean J. Breed. Sci.

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Sybeans have been grown by plant breeding for decades. As soybeans have very limited genetic variation, it is difficult for soybean breeders to find new genetic resources for abiotic stressors. Recently, soybeans have been exposed to flooding stress from intensive summer rainfall owing to climate change. Glycine soja, a wild soybean, is known to have greater genetic variation and greater resistance to a variety of biotic and abiotic stresses than ordinary soybeans. In this study, high-throughput transcriptome analysis was performed using flood-treated Glycine soja. Differentially expressed genes (DEGs) were analyzed using reads mapped to reference sequences, and gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed using selected DEGs. In addition, RT-qPCR analysis was performed to further analyze the expression of specific candidate genes. Several novel genes that could explain various mechanisms related to water stress were identified as related transcripts and adaptation mechanisms through cell wall expansion, alcoholic fermentation under anaerobic conditions, and structural changes. In addition, most of the isoflavonoid daidzein pathway genes exhibited upregulated expression under flooding stress. Interestingly, expression of the DIR (dirigent protein 1-like) gene, which is known to decrease in response to flooding stress in soybeans (Glycine max), was upregulated in Glycine soja. The expression of DIR revealed that DIR may play a key role in conferring flooding stress resistance in Glycine soja. This study provides useful information regarding the genes and comprehensive adaptation mechanisms related to flooding stress tolerance that can be utilized for cultivated soybeans through the Korean wild soybean.

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Expression Analysis of Pre-Harvest Sprouting Tolerant Korean Wheat via Transcriptomic Analysis

Cited by 2 publications

References 80 publications

Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (Triticum aestivum)

Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (Triticum aestivum)

Transcriptome Analysis for Flooding Stress-related Gene Identification inGlycine soja

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