Expression analysis of Rho GTPase-related lncRNAs in breast cancer

Nicknam, Amir; Pour, Sahar Khojasteh; Hashemnejad, Mohammad Amin; Hussen, Bashdar Mahmud; Safarzadeh, Arash; Eslami, Solat; Taheri, Mohammad; Ghafouri‐Fard, Soudeh; Jamali, Elena

doi:10.1016/j.prp.2023.154429

Cited by 5 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The RHO GTPases are related to endosomal membrane trafficking in various cell lines [32] and sEV secretion in Oli-neu cells [33], which is responsible for the regulation of cancer invasion and progression [34]. Recently, it was discovered that the RHO GTPase family and its related lncRNAs, including RAD51-AS1 and DANCR, participated in BC carcinogenesis [35]. The Notch signalling pathway is one of the well-known factors in BC progression [36].…”

Section: Discussionmentioning

confidence: 99%

Comparison Study of Small Extracellular Vesicle Isolation Methods for Profiling Protein Biomarkers in Breast Cancer Liquid Biopsies

Lee,

Ni,

Wasinger

et al. 2023

IJMS

View full text Add to dashboard Cite

Small extracellular vesicles (sEVs) are an important intercellular communicator, participating in all stages of cancer metastasis, immunity, and therapeutic resistance. Therefore, protein cargoes within sEVs are considered as a superior source for breast cancer (BC) biomarker discovery. Our study aimed to optimise the approach for sEV isolation and sEV proteomic analysis to identify potential sEV protein biomarkers for BC diagnosis. sEVs derived from BC cell lines, BC patients’ plasma, and non-cancer controls were isolated using ultracentrifugation (UC), a Total Exosome Isolation kit (TEI), and a combined approach named UCT. In BC cell lines, the UC isolates showed a higher sEV purity and marker expression, as well as a higher number of sEV proteins. In BC plasma samples, the UCT isolates showed the highest proportion of sEV-related proteins and the lowest percentage of lipoprotein-related proteins. Our data suggest that the assessment of both the quantity and quality of sEV isolation methods is important in selecting the optimal approach for the specific sEV research purpose, depending on the sample types and downstream analysis.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison Study of Small Extracellular Vesicle Isolation Methods for Profiling Protein Biomarkers in Breast Cancer Liquid Biopsies

Lee,

Ni,

Wasinger

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…In this context, NORAD levels also correlate with RHOA and RAD51 antisense RNA 1 (RAD51-AS1) expression. NORAD is significantly increased in BC tumors compared to adjacent normal tissue, presenting a great specificity value for segregation between BC and non-tumoral tissues [51].…”

Section: Norad-regulated Signaling Pathways Via Ncrna Spongingmentioning

confidence: 99%

NORAD-Regulated Signaling Pathways in Breast Cancer Progression

Capela,

Tavares-Marcos,

Estima-Arede

et al. 2024

Cancers

View full text Add to dashboard Cite

Long non-coding RNA activated by DNA damage (NORAD) has recently been associated with pathologic mechanisms underlying cancer progression. Due to NORAD’s extended range of interacting partners, there has been contradictory data on its oncogenic or tumor suppressor roles in BC. This review will summarize the function of NORAD in different BC subtypes and how NORAD impacts crucial signaling pathways in this pathology. Through the preferential binding to pumilio (PUM) proteins PUM1 and PUM2, NORAD has been shown to be involved in the control of cell cycle, angiogenesis, mitosis, DNA replication and transcription and protein translation. More recently, NORAD has been associated with PUM-independent roles, accomplished by interacting with other ncRNAs, mRNAs and proteins. The intricate network of NORAD-mediated signaling pathways may provide insights into the potential design of novel unexplored strategies to overcome chemotherapy resistance in BC treatment.

show abstract

“…LncRNA NORAD has also been shown to promote lung cancer cell proliferation, invasion, and migration through CXCR4 and RhoA/ROCK signaling pathway ( 125 ). According to latest bioinformatics research, lncRNA NORAD can also promote breast cancer progression through the Rho GTPase pathway ( 126 ). However, the research about lncRNAs and entosis in other tumors is still elusive.…”

Section: Entosismentioning

confidence: 99%

LncRNAs and regulated cell death in tumor cells

Wen

Xia

et al. 2023

Front. Oncol.

View full text Add to dashboard Cite

Regulated Cell Death (RCD) is a mode of cell death that occurs through drug or genetic intervention. The regulation of RCDs is one of the significant reasons for the long survival time of tumor cells and poor prognosis of patients. Long non-coding RNAs (lncRNAs) which are involved in the regulation of tumor biological processes, including RCDs occurring on tumor cells, are closely related to tumor progression. In this review, we describe the mechanisms of eight different RCDs which contain apoptosis, necroptosis, pyroptosis, NETosis, entosis, ferroptosis, autosis and cuproptosis. Meanwhile, their respective roles in the tumor are aggregated. In addition, we outline the literature that is related to the regulatory relationships between lncRNAs and RCDs in tumor cells, which is expected to provide new ideas for tumor diagnosis and treatment.

show abstract

Expression analysis of Rho GTPase-related lncRNAs in breast cancer

Cited by 5 publications

References 21 publications

Comparison Study of Small Extracellular Vesicle Isolation Methods for Profiling Protein Biomarkers in Breast Cancer Liquid Biopsies

Comparison Study of Small Extracellular Vesicle Isolation Methods for Profiling Protein Biomarkers in Breast Cancer Liquid Biopsies

NORAD-Regulated Signaling Pathways in Breast Cancer Progression

LncRNAs and regulated cell death in tumor cells

Contact Info

Product

Resources

About