Expression analysis of serum microRNAs in idiopathic pulmonary fibrosis

Li, Ping; Li, Juan; Chen, Tengfei; Wang, Huaqi; Chu, Heying; Chang, Jingxia; Zang, Wenqiao; Wang, Yuanyuan; Ma, Yue; Du, Yuwen; Zhao, Guoqiang; Zhang, Guojun

doi:10.3892/ijmm.2014.1712

Cited by 54 publications

(41 citation statements)

References 32 publications

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“…Levels of miR-21 and miR-155 were significantly elevated in IPF patient serum (Li et al, 2013(Li et al, , 2014, consistent with findings from the cell-based mouse and human studies discussed earlier (Pottier et al, 2009;Liu et al, 2010;Yamada et al, 2013). The upregulation of serum miR-21 was confirmed as a predictor of poor prognosis by two other groups Makiguchi et al, 2016).…”

Section: Clinical Use Of Mirnas In Respiratory Disease Mirnas As Biomsupporting

confidence: 86%

The role of microRNAs in chronic respiratory disease: recent insights

Stolzenburg¹,

Harris²

2017

Biological Chemistry

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Chronic respiratory diseases encompass a group of diverse conditions affecting the airways, which all impair lung function over time. They include cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF), chronic obstructive pulmonary disease (COPD) and asthma, which together affect hundreds of millions of people worldwide. MicroRNAs (miRNAs), a class of small non-coding RNAs involved in post-transcriptional gene repression, are now recognized as major regulators in the development and progression of chronic lung disease. Alterations in miRNA abundance occur in lung tissue, inflammatory cells, and freely circulating in blood and are thought to function both as drivers and modifiers of disease. Their importance in lung pathology has prompted the development of miRNA-based therapies and biomarker tools. Here, we review the current literature on miRNA expression and function in chronic respiratory disease and highlight further research that is needed to propel miRNA treatments for lung disorders towards the clinic.

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Section: Clinical Use Of Mirnas In Respiratory Disease Mirnas As Biomsupporting

confidence: 86%

The role of microRNAs in chronic respiratory disease: recent insights

Stolzenburg¹,

Harris²

2017

Biological Chemistry

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“…The detection of specific miRNA alterations in serum samples of IPF patients suggests that miRNA could be used as a noninvasive screening tool for IPF in routine clinical practice, establishing more reliable biomarkers [126,128]. Novel treatment strategies based on epigenetic drugs, such as 5-aza-2′-deoxycytidine and decitabine [129], or exogenous administration of miRNAs, could also prove beneficial for management of IPF patients.…”

Section: Biomarkers In Ipf and Osamentioning

confidence: 99%

Idiopathic pulmonary fibrosis and sleep disorders: no longer strangers in the night

et al. 2015

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The prevalence of obstructive sleep apnoea (OSA) is continuously increasing in patients with idiopathic pulmonary fibrosis (IPF) and, for the first time, the recent IPF guidelines recognise OSA as an important associated comorbidity that can affect patient's survival. Thus, it becomes conceivable that clinicians should refer patients with newly diagnosed IPF to sleep centres for the diagnosis and treatment of OSA as well as for addressing issues regarding the reduced compliance of patients with continuous positive airway pressure therapy. The discovery of biomarkers common to both disorders may help early diagnosis, institution of the most appropriate treatment and follow-up of patients. Better understanding of epigenetic changes may provide useful information about pathogenesis and, possibly, development of new drugs for a dismal disease like IPF. @ERSpublications It is now believed that IPF and sleep disorders can coexist in the same patient

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“…44 MicroRNAs have been implicated in the development of fibrosis via extracellular matrix and proliferative changes especially in liver, kidney and lung fibrotic diseases. 5,45,46 In this study, we aimed to ascertain the role of microRNAs in fibroblast function, using human pterygium as a model. Here we found miR-215, a conserved microRNA previously unreported in pterygium, to regulate cell cycling in pterygium-derived fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-215 Regulates Fibroblast Function: Insights from a Human Fibrotic Disease

Lan

Chen

Tong³

2015

Cell Cycle

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MicroRNAs are implicated in the regulation of gene expression via various mechanisms in health and disease, including fibrotic processes. Pterygium is an ocular surface condition characterized by abnormal fibroblast proliferation and matrix deposition. We aimed to investigate the role of microRNAs in pterygium and understand the relevant cellular and molecular mechanisms. To achieve this objective, a combination of approaches using surgically excised paired human pterygium and conjunctival tissues as well as cultured primary fibroblast cells from tissue explants were evaluated. Fibroblast dysfunction has been shown to play a central role in pterygium pathology. Here we show that miR-215, among a few others, was down-regulated (2-fold) in pterygium compared to control, and this was consistent in microarray, real-time PCR and fluorescent in-situ hybridization. The effects of increased miR-215 were investigated by adding exogenous miR-215 to fibroblasts, and this showed a decrease in cell proliferation but no significant apoptosis compared to control. Further cell cycle analysis showed that miR-215 depressed progression of cells at G1/S as well as G2/M. A few cell cycle related transcripts were downregulated (2.2-4.5-fold) on addition of miR-215: Mcm3, Dicer1, Cdc25A, Ick, Trip13 and Mcm10. Theoretic binding energies were used to predict miR-215 binding targets and luciferase reporter studies confirmed Mcm10 and Cdc25A as direct targets. In summary, mir-215 could play a role in inhibiting fibroblast proliferation in ocular surface conjunctiva. Dampening of this mir-215 could result in increased fibroblast cell cycling and proliferation, with possibly increased fibroblastic production of matrix, inducing pterygium formation.

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Expression analysis of serum microRNAs in idiopathic pulmonary fibrosis

Cited by 54 publications

References 32 publications

The role of microRNAs in chronic respiratory disease: recent insights

The role of microRNAs in chronic respiratory disease: recent insights

Idiopathic pulmonary fibrosis and sleep disorders: no longer strangers in the night

MicroRNA-215 Regulates Fibroblast Function: Insights from a Human Fibrotic Disease

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