Expression analysis of the peroxiredoxin gene family during early development in Xenopus laevis

Shafer, Maxwell E.R.; Willson, Jessica A.; Damjanovski, Sashko

doi:10.1016/j.gep.2011.08.007

Cited by 12 publications

(7 citation statements)

References 42 publications

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“…). Similar to a previous report in Xenopus laevis , we also observed that silkworm Prx4 was expressed in neural regions including the brain and somites and in the olfactory organ (Shafer et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…Thus, we performed immunohistochemical analyses to localize BmPrx4 in head tissues of silkworm larvae. Prx has been detected in the olfactory system of some species, such as Xenopus laevis and the mouse (Godoy et al ., ; Shafer et al ., ). Our results also showed that BmPrx4 is distributed in the olfactory system of the silkworm, suggesting that the olfactory system might require BmPrx4 to protect itself from oxidative damage caused by harmful chemicals.…”

Section: Discussionmentioning

confidence: 97%

“…Mutated forms of Prx enzymes have been observed in the substantia nigra of Parkinson's disease in animal models as well as human subjects, and expression of several peroxiredoxins was also observed in the olfactory placode. These results demonstrate a neuroprotective role of Prxs (Fang et al ., ; Qu et al ., ; Lee et al ., ; Shafer et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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Molecular cloning and characterization of peroxiredoxin 4 involved in protection against oxidative stress in the silkworm Bombyx mori

Shi

et al. 2012

Insect Molecular Biology

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Peroxiredoxins (Prxs) are a ubiquitous family of proteins that play important roles in insects in protection against oxidative stress through the detoxification of cellular peroxides. Here, we describe the cloning and characterization of a Prx4 cDNA of the silkworm Bombyx mori (BmPrx4). The BmPrx4 gene has an open reading frame of 744 bp encoding 248 amino acids and a conserved motif, VCP, involved in its presumed redox functions. The heterologously expressed proteins of the gene in Escherichia coli showed antioxidant activity, removed hydrogen peroxide and protected DnA. Western blotting analysis showed the presence of BmPrx4 in the haemolymph, suggesting that the protein is secretable. Moreover, BmPrx4 was expressed at all developmental stages. The expression level of BmPrx4 was relatively low during the feeding stage but high at the wandering stage. BmPrx4 was induced by quercetin or temperature stress. Immunohistochemical analysis revealed that BmPrx4 is present in the brain, neurones and olfactory organ of the head in silkworms. Overall, our results indicate that the expression profile of BmPrx4 correlates well with protection from oxidative damage. Our data provide clues for the development of control technology for agricultural and forestry pests as the silkworm is a representative of lepidopteran pests.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

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Molecular cloning and characterization of peroxiredoxin 4 involved in protection against oxidative stress in the silkworm Bombyx mori

Shi

et al. 2012

Insect Molecular Biology

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“…The consumption of yolk stores during a specific period of development involves governing regulatory signals and controlling ROS by-products (Shafer et al 2011). Most antioxidant enzymes such as catalase and glutathione peroxidase are expressed at high levels during yolk consumption.…”

Section: Discussionmentioning

confidence: 99%

Peroxiredoxin 6 from cuttlefish (Sepiella maindroni): molecular characterisation during development and the response to thermal stress

Song

Wang

2017

Int Aquat Res

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The aim of this work was to investigate the function of the peroxiredoxin family member Peroxiredoxin 6 in Sepiella maindroni (Smprx6) during embryonic development and the response to thermal stress. The 1064 bp full-length coding sequence of Smprx6 includes an open reading frame of 660 bp encoding a polypeptide of 219 amino acids with a predicted molecular mass of 24.4 kDa. The deduced protein sequence shares significant homology with other reported Prx6 enzymes. Phylogenetic analysis classified SmPrx6 into the 1-Cys-Prx cluster, and quantitative real-time PCR (qPCR) demonstrated constitutive mRNA expression in all tested tissues and during five different embryonic developmental stages, as previously documented in other cephalopods. Thermal stress induced Smprx6 expression, which peaked at 6 h then returned to control levels. Recombinant SmPrx6 was active at different concentrations and was thiol-dependant. The results suggest that SmPrx6 plays a major role in the development of Sepiella maindroni and its response to oxidative and thermal stress.

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“…Additional specificities are revealed by in situ hybridization. Specifically, prx1 is expressed in anterior structures, and prx4, 5 and 6 expression is preferentially detected in somites [119]. This pattern of expression is not completely conserved between Xenopus and other vertebrates.…”

Section: Embryonic Developmentmentioning

confidence: 93%

Hydrogen Peroxide and Redox Regulation of Development

Rampon¹,

Volovitch²,

Joliot³

et al. 2018

Preprint

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Reactive oxygen species (ROS), which were originally classified as exclusively deleterious compounds, have gained increasing interest in the recent years given their action as bona fide signalling molecules. The main target of ROS action is the reversible oxidation of cysteines, leading to the formation of disulfide bonds, which modulate protein conformation and activity. ROS endowed with signalling properties are mainly produced by NADPH oxidases at the plasma membrane, but their action also involves a complex machinery of multiple redox-sensitive protein families that differs in their subcellular localization and their activity. Given that the levels and distribution of ROS are highly dynamic in part due to their limited stability, the development of various fluorescent ROS sensors, some of which are quantitative (ratiometric), represents a clear breakthrough in the field and have been adapted to both ex vivo and in vivo applications. The physiological implication of ROS signalling will be presented mainly in the frame of morphogenetics processes, embryogenesis, regeneration, and stem cell differentiation. Gain and loss of function as well as pharmacological strategies have demonstrated the wide but specific requirement of ROS signalling at multiple stages of these processes and its intricate relationship with other well-known signalling pathways.

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Expression analysis of the peroxiredoxin gene family during early development in Xenopus laevis

Cited by 12 publications

References 42 publications

Molecular cloning and characterization of peroxiredoxin 4 involved in protection against oxidative stress in the silkworm Bombyx mori

Molecular cloning and characterization of peroxiredoxin 4 involved in protection against oxidative stress in the silkworm Bombyx mori

Peroxiredoxin 6 from cuttlefish (Sepiella maindroni): molecular characterisation during development and the response to thermal stress

Hydrogen Peroxide and Redox Regulation of Development

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