Expression analysis of the PMP22 gene in glioma and osteogenic sarcoma cell lines

Abstract

Previously we reported the amplification of the peripheral myelin protein 22 (PMP22) gene in cell lines of human osteogenic and glioma tumors. PMP22 normally is expressed at high levels in Schwann cells of the peripheral nervous system and is suggested to function as a structural protein of the myelin sheath. One of the most common inherited peripheral neuropathies, Charcot-Marie-Tooth Type 1A (CMT1A), is associated with a duplication of a 1.5-Mb DNA region on chromosome 17p11.2 - p12 containing PMP22. On the other hand, PMP22 is identical to gas3, whose expression is induced in growth-arresting NIH3T3-fibroblasts and is thought to play a role in cell proliferation. The precise role of gas3/PMP22 remains to be determined. Here we show that in the tumor cell lines RH30 and SF763 the amplified region including PMP22 comprises the whole 1.5-Mb CMT1A region. We could prove expression of PMP22 by reverse transcriptase-polymerase chain reaction (RT-PCR) and discovered an unusual PMP22 transcript in these tumor cell lines. Western blot analyses resulted in detection of a 22-kDa protein by the PMP22-specific antibody 558/2 and in exclusion of myelin protein zero (MPZ) expression in these cell lines.

MiR-139-5p negatively regulates PMP22 to repress cell proliferation by targeting the NF-κB signaling pathway in gastric cancer

“…Most research on PMP22 proteins has focused on the nervous system. However, recent several studies show that PMP22 regulates tumor development, metastasis, and invasion in different cancers [8][9][10][11][12][13][14][15][16][17][18][19]21]. At present, there is no clear evidence of PMP22 acting as an oncogene or as a tumor suppressor gene.…”

“…International Publisher that PMP22 is a potential tumor suppressor [8][9][10][11][12][13], whereas some observations suggest a potential oncogenic function of PMP22 [14][15][16][17][18][19][20][21][22]. Few studies have examined the function and regulation of PMP22 in gastric cancer.…”

PMP22 Regulates Self-Renewal and Chemoresistance of Gastric Cancer Cells

“…PMP22 participates in the oncogenesis, progression, and metastasis of osteosarcoma (23)(24)(25)(26). Furthermore, high-level PMP22 amplification was observed in high-grade glioma (27,28). In the current study, we revealed for the first time the role of PMP22 in gastric cancer in potentially mediating selfrenewal and chemoresistance in gastric cancer cells.…”

“…The precise expression and function of PMP22 in tumors remain to be clarified. Several studies indicate that PMP22 is a potential tumor suppressor (21,22), whereas some observations suggest a potential oncogenic function of PMP22 in tumors (23)(24)(25)(26)(27)(28). Studies on the role of PMP22 in regulating gastric cancer have not been reported, and we describe here that PMP22 is required for both self-renewal and chemoresistance in gastric cancer.…”

Charcot-Marie-Tooth 1A concurrent with anaplastic ependymoma in a toddler: when an acute event unmasks a chronic condition

“…Amplification of the PMP22 expression has been identified in glioma and osteosarcoma cell lines. 12,13 Nevertheless, no preclinical or clinical data about the role of the PMP22 in ependymal cells exists. Therefore, no link between ependymoma and PMP22 gene can be drawn or speculated.…”