Expression analysis of transcripts responsive to osmotic stress in Deschampsia antarctica Desv.

Lee, Jung-Eun; Choi, Yongrok; Noh, Eun Kyeung; Park, Soo‐Jin; Park, Hyun; Kim, Ji Hyun; Kim, Il-Chan; Yim, Joung Han

doi:10.1007/s13258-013-0166-5

Cited by 4 publications

(4 citation statements)

References 34 publications

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“…Interestingly, we noticed that the induction of higher P5CS expression preceded the accumulation of proline under drought stress. Similarly, P5CS gene upregulation under drought stress has also been observed in wheat [34][35][36][37]. It is clear that plants overexpressing P5CS accumulate more proline than the control plants and are tolerant to osmotic stress [38].…”

Section: Drought Affects Gene Expressionmentioning

confidence: 72%

Comparative Molecular and Metabolic Profiling of Two Contrasting Wheat Cultivars under Drought Stress

Fadoul

Rivas

Neumann

et al. 2021

IJMS

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Drought is one of the most important threats to plants and agriculture; therefore, understanding of the mechanisms of drought tolerance is crucial for breeding of new tolerant varieties. Here, we assessed the effects of a long-term water deficit stress simulated on a precision phenotyping system on some morphological criteria and metabolite traits, as well as the expression of drought associated transcriptional factors of two contrasting drought-responsive African wheat cultivars, Condor and Wadielniel. The current study showed that under drought stress Wadielniel exhibits significant higher tillering and height compared to Condor. Further, we used gas chromatography and ultra-high performance liquid chromatography mass-spectrometry to identify compounds that change between the two cultivars upon drought. Partial least square discriminant analysis (PLS-DA) revealed that 50 metabolites with a possible role in drought stress regulation were significantly changed in both cultivars under water deficit stress. These metabolites included several amino acids, most notably proline, some organic acids, and lipid classes PC 36:3 and TAG 56:9, which were significantly altered under drought stress. Here, the results discussed in the context of understanding the mechanisms involved in the drought response of wheat cultivars, as the phenotype parameters, metabolite content and expression of drought associated transcriptional factors could also be used for potential crop improvement under drought stress.

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Section: Drought Affects Gene Expressionmentioning

confidence: 72%

Comparative Molecular and Metabolic Profiling of Two Contrasting Wheat Cultivars under Drought Stress

Fadoul

Rivas

Neumann

et al. 2021

IJMS

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“…We observed increased proline levels with increasing salinity. Other studies have reported increases in proline concentrations in D. antarctica in response to cold stress and changes in day length (Bravo et al 2001), and under osmotic stress conditions (Lee et al 2014). This suggests that free proline could contribute to the osmotic adjustment of D. antarctica and hence its survival in the environmental conditions of the Antarctic region.…”

Section: Discussionmentioning

confidence: 86%

“…2001), and under osmotic stress conditions (Lee et al . 2014). This suggests that free proline could contribute to the osmotic adjustment of D. antarctica and hence its survival in the environmental conditions of the Antarctic region.…”

Section: Discussionmentioning

confidence: 99%

Salt tolerance traits in Deschampsia antarctica Desv.

et al. 2016

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Deschampsia antarctica Desv. (Poaceae) grows in coastal habitats in the Maritime Antarctic where it is often exposed to sea spray. Salt crystals have been observed on the surface of leaves in plants treated with high NaCl. We investigated if D. antarctica is a salt tolerant species that allows sodium ions to diffuse into the root where a salt overly sensitive (SOS) system extrudes Na+ from root cells and facilitates its movement through the xylem up to the leaves. Leaf epidermis, physiological parameters and sodium transporters in D. antarctica plants exposed to NaCl were studied over 21 days. Epidermal scanning electron microscopy showed trichome induction in the leaves of salt treated plants. In addition, salt treated plants showed increased sodium and proline levels with a concomitant increased expression of SOS genes (1 and 3). These results indicate that Na+ is taken up by the roots of D. antarctica and transported to the leaves. The sodium flux may be controlled by SOS1 activity. Up-regulation of the SOS1 gene may be involved in the increased sodium levels observed in the leaves of salt treated plants. Trichomes may also be involved in sodium exudation through the leaves under saline conditions.

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“…D. antarctica exhibits specialized morphological properties, such as distinct leaf anatomy and chloroplast ultra-structure, that influence its photosynthetic efficiency in extreme environments ( Giełwanowska et al, 2005 ; Sáez et al, 2019 ), and its tolerance to ultraviolet B-induced oxidative stress is enhanced by a combination of phenolic molecules synthesis and the activation of both enzymatic and non-enzymatic antioxidant systems ( Köhler et al, 2017 ). The transcriptome responses of D. antarctica have been examined under various abiotic stress conditions ( Lee et al, 2013 , 2014 ). Increases in ice recrystallization inhibition protein ( DaIRIP ) expression and recrystallization inhibition activity in response to freezing stress may contribute to the cryotolerance of D. antarctica ( John et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice

et al. 2021

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The Antarctic flowering plant Deschampsia antarctica is highly sensitive to climate change and has shown rapid population increases during regional warming of the Antarctic Peninsula. Several studies have examined the physiological and biochemical changes related to environmental stress tolerance that allow D. antarctica to colonize harsh Antarctic environments; however, the molecular mechanisms of its responses to environmental changes remain poorly understood. To elucidate the survival strategies of D. antarctica in Antarctic environments, we investigated the functions of actin depolymerizing factor (ADF) in this species. We identified eight ADF genes in the transcriptome that were clustered into five subgroups by phylogenetic analysis. DaADF3, which belongs to a monocot-specific clade together with cold-responsive ADF in wheat, showed significant transcriptional induction in response to dehydration and cold, as well as under Antarctic field conditions. Multiple drought and low-temperature responsive elements were identified as possible binding sites of C-repeat-binding factors in the promoter region of DaADF3, indicating a close relationship between DaADF3 transcription control and abiotic stress responses. To investigate the functions of DaADF3 related to abiotic stresses in vivo, we generated transgenic rice plants overexpressing DaADF3. These transgenic plants showed greater tolerance to low-temperature stress than the wild-type in terms of survival rate, leaf chlorophyll content, and electrolyte leakage, accompanied by changes in actin filament organization in the root tips. Together, our results imply that DaADF3 played an important role in the enhancement of cold tolerance in transgenic rice plants and in the adaptation of D. antarctica to its extreme environment.

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Expression analysis of transcripts responsive to osmotic stress in Deschampsia antarctica Desv.

Cited by 4 publications

References 34 publications

Comparative Molecular and Metabolic Profiling of Two Contrasting Wheat Cultivars under Drought Stress

Comparative Molecular and Metabolic Profiling of Two Contrasting Wheat Cultivars under Drought Stress

Salt tolerance traits in Deschampsia antarctica Desv.

Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice

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