Expression and 7-day time course of circulating microRNAs in septic patients treated with nephrotoxic antibiotic agents

Petejová, Naděžda; Martínek, Arnošt; Zadrazil, J; Klementa, Viktor; Přibylová, Lenka; Briš, Radim; Káňová, Marcela; Šigutová, Radka; Kacířová, Ivana; Švagera, Zdeněk; Bace, E.; Stejskal, David

doi:10.1186/s12882-022-02726-6

Cited by 7 publications

(4 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR-15a-5p at day 7 of gentamicin treatment provides good discrimination between AKI and non-AKI. miR-155-5p and miR-192-5p positively correlate with creatinine and neutrophil gelatinase-associated lipokalin in patients receiving vancomycin (210). These data suggest that miRNAs expression might be modulated by antimicrobials, and may serve as diagnostic markers in sepsis patients receiving nephrotoxic antibiotics.…”

Section: Other Mirnasmentioning

confidence: 74%

Modes of action and diagnostic value of miRNAs in sepsis

et al. 2022

View full text Add to dashboard Cite

Sepsis is a clinical syndrome defined as a dysregulated host response to infection resulting in life-threatening organ dysfunction. Sepsis is a major public health concern associated with one in five deaths worldwide. Sepsis is characterized by unbalanced inflammation and profound and sustained immunosuppression, increasing patient susceptibility to secondary infections and mortality. microRNAs (miRNAs) play a central role in the control of many biological processes, and deregulation of their expression has been linked to the development of oncological, cardiovascular, neurodegenerative and metabolic diseases. In this review, we discuss the role of miRNAs in sepsis pathophysiology. Overall, miRNAs are seen as promising biomarkers, and it has been proposed to develop miRNA-based therapies for sepsis. Yet, the picture is not so straightforward because of the versatile and dynamic features of miRNAs. Clearly, more research is needed to clarify the expression and role of miRNAs in sepsis, and to promote the use of miRNAs for sepsis management.

show abstract

Section: Other Mirnasmentioning

confidence: 74%

Modes of action and diagnostic value of miRNAs in sepsis

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Previous data indicate that miR-155-5p exerts a signi cant in uence on multiple pathways associated with sepsis and renal damage, and a direct relationship exists between the activation of miR-155-5p and increased expression of pro-in ammatory IL-6 cytokine and chemoattractant IL-8 cytokine [23,24]. Earlier studies showed that up-regulation of miR-155 has cardioprotective properties [25].…”

Section: Discussionmentioning

confidence: 99%

Postmortem analyses of myocardial microRNA expression in sepsis

Lehto,

Saukko,

Säkkinen

et al. 2024

Preprint

View full text Add to dashboard Cite

Background Sepsis can lead to myocardial depression, playing a significant role in sepsis pathophysiology, clinical care, and outcome. To gain more insight into the pathophysiology of the myocardial response in sepsis, we investigated the expression of microRNA in myocardial autopsy specimens in critically ill deceased with sepsis and non-septic controls. Materials and methods In this retrospective observational study, we obtained myocardial tissue samples collected during autopsy from adult patients deceased with sepsis (n = 15) for routine histological examination. We obtained control myocardial tissue specimens (n = 15) from medicolegal autopsies of cadavers whose cause of death was injury or who were found dead at home and the cause of death was coronary artery disease with sudden cardiac arrest. RNA was isolated from formalin-fixed paraffin- embedded (FFPE) cardiac samples using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Invitrogen). Differentially expressed miRNAs were identified using edgeR v3.32. MicroRNA was considered up- or down-regulated if the false discovery rate was < 0.05 and logarithmic fold change (log2FC) ≥ 1 for up-regulated or log2FC ≤ -1 for down-regulated miRNAs. The mean difference and 95% confidence interval (CI) was calculated for normalized read counts. Predicted miRNA targets were retrieved using Ingenuity Pathway Analysis (IPA) software, and pathway enrichment and classification were performed using PantherDB. Results Differential expression analysis identified a total of 32 miRNAs in the myocardial specimens. Eight miRNAs had a significant change in the mean difference based on the 95% CI, with the largest increase in mean counts in septic samples with hsa-miR-12136 and the highest fold change with hsa-miR-146b-5p. The threshold for down-regulated miRNAs in sepsis compared to controls was obtained with hsa-miR-144-5p and hsa-miR-451a, with the latter having the largest decrease in mean counts and fold decrease. Conclusions Several regulatory miRNAs were up- or down-regulated in the myocardial tissue of patients deceased with sepsis compared to non-septic subjects. The predicted target genes of miRNAs are associated with biological functions related to cardiovascular functions, cell viability, cell adhesion, and regulation of inflammatory and immune response.

show abstract

“…Multiple studies have demonstrated the potential value of microRNA as a diagnostic tool for septic AKI. Some -15a-5p, microR-22-3p, and microR-452 (Liu et al 2020b, Petejova et al 2022. The expression of microR-574-5p can differentiate the AKI occurrence from septic patients, indicating the predictive potential of microRNAs in the clinic (Liu et al 2021).…”

Section: Discussionmentioning

confidence: 99%