Expression and antimicrobial activity analysis of dermaseptin B1 recombinant peptides in tobacco transgenic plants

Khademi, Mitra; Nazarian-Firouzabadi, Farhad

doi:10.29252/pgr.6.1.139

Cited by 3 publications

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“…) . Khademi et al, 2019 .) source 3%) and combation of incoulation time with bacteria strain (b1: A4 strain with 5 min incoulation, b2: A4 strain with 10 min incoulation, b3: ATTC strain with 5 min incoulation and b4: ATTC strain with 10 min incoulation…”

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confidence: 98%

Induction of DrsB1-CBDAvr4 Recombinant Protein in Hairy and Adventitious Roots of T1 Transgenic Plants

Khademi

Varasteh-Shams

Nazarian-Firouzabadi

2022

pgr

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Hairy and adventitious roots are efficient systems for expressing recombinant proteins. In the present study, the amount of DrsB1-CBDAvr4 recombinant protein in hairy and adventitious root systems was compared. To this end, the effect of different factors on the optimization of culture conditions to obtain adventitious and hairy roots was evaluated in three separate experiments by assessment of biomass production in T1 transgenic plants expressing DrsB1-CBDAvr4 recombinant protein. The efficacy of Agrobacterium rhizogenesis in producing hairy roots was ensured using rolC gene specific primers. The insertion of DrsB1-CBDAvr4 recombinant peptide transgene in the genome of hairy and adventitious roots was confirmed by PCR analysis. Also, the level of DrsB1-CBDAvr4 protein was measured in hairy and adventitious roots by ELISA analysis. Analysis of the variance of data showed that the highest number of roots and the longest roots were obtained in MS media supplemented with 1 mg/L NAA and 0.5 mg/L IBA. The results of adventitious root biomass showed that liquid MS medium containing 1 mg/L of NAA hormone had a significant effect (P <0.01) on biomass production. More biomass was obtained in MS medium supplemented with 1mg/L NAA, whereas a lower fresh and dry weight was obtained in a 1/4 MS medium with no NAA. The results also showed that ATCC15834 strain with MS media supplemented with 3% sucrose, 10 minutes inoculation time was high efficiency to induce hairy roots in tobacco plants. The results of ELISA analysis showed that clones obtained from both roots showed a significant difference in terms of total protein content. The amount of recombinant protein from hairy roots was much higher than that of adventitious roots.

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confidence: 98%

Induction of DrsB1-CBDAvr4 Recombinant Protein in Hairy and Adventitious Roots of T1 Transgenic Plants

Khademi

Varasteh-Shams

Nazarian-Firouzabadi

2022

pgr

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Preserving Quality of Button Mushrooms (Agaricus bisporus) Using Nano-chitosan-Aloe Vera Coating with Tomato Seed Protein Hydrolyzate

Falahati,

Ariaii,

Khoshkhoo

et al. 2024

Preprint

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Button mushroom (Agaricus bisporus) is a widely consumed edible mushroom, but its quality deteriorates rapidly after harvest. Therefore, the use of edible coatings with natural preservative compounds is essential to delay and reduce microbial growth and maintain mushroom quality. This study examined the effects of a nano-chitosan (NC)-aloe vera (AV) edible coating combined with tomato seed protein hydrolyzate (TPH) as a natural preservative on the chemical, microbial, and organoleptic properties of button mushrooms. TPH was initially prepared using the enzyme Alcalase. Five edible films containing NC, NC-AV, and varying concentrations of TPH (0%, 0.5%, 1%, 1.5%) were then produced. The shelf lives of coated mushrooms were evaluated during 16 days of refrigerated storage (4 ± 1°C). Results showed that the TPH had high levels of protein (90.16%), hydrophobic amino acids (31.78%), and aromatic amino acids (11.74%). The produced films exhibited high antioxidant and antimicrobial activities, with improved results observed at increased concentrations of TPH. Compared to uncoated mushrooms, the nanocomposite coatings significantly reduced physicochemical changes, quality degradation, and microbial spoilage. Increased concentrations of TPH further enhanced browning inhibition, free radical scavenging, and microbial spoilage reduction (p < 0.05). Sensory evaluation indicated that the sample containing 1.5% TPH had the highest overall acceptance. The use of a NC-AV composite coating containing TPH can be an effective method for extending the shelf life of white button mushrooms.

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Identification, Isolation and Expression Analysis of Hevein gene Family in Barley (Hordeum vulgar)

Derikvand

Sohrabi

et al. 2022

pgr

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Expression and antimicrobial activity analysis of dermaseptin B1 recombinant peptides in tobacco transgenic plants

Cited by 3 publications

References 0 publications

Induction of DrsB1-CBDAvr4 Recombinant Protein in Hairy and Adventitious Roots of T1 Transgenic Plants

Induction of DrsB1-CBDAvr4 Recombinant Protein in Hairy and Adventitious Roots of T1 Transgenic Plants

Preserving Quality of Button Mushrooms (Agaricus bisporus) Using Nano-chitosan-Aloe Vera Coating with Tomato Seed Protein Hydrolyzate

Identification, Isolation and Expression Analysis of Hevein gene Family in Barley (Hordeum vulgar)

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