Expression and characterization of a new isoform of the 9 kDa allergenic lipid transfer protein from tomato (variety San Marzano)

Volpicella, Mariateresa; Leoni, Claudia; Fanizza, Immacolata; Rinalducci, Sara; Placido, Antonio; Ceci, Luigi R.

doi:10.1016/j.plaphy.2015.07.019

Cited by 8 publications

(11 citation statements)

References 42 publications

(50 reference statements)

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“…Notably a tomato LTP was found to bind L-α-palmitoyl-lysophosphatidylcholine (C16). This complex was stable even after thermal treatments with up to 105°C ( 22 ).…”

Section: Structural Interaction Of Food-derived Lipids With Nsltpsmentioning

confidence: 99%

Interaction of Non-Specific Lipid-Transfer Proteins With Plant-Derived Lipids and Its Impact on Allergic Sensitization

Scheurer

Schülke

2018

Front. Immunol.

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Non-specific lipid-transfer proteins (nsLTPs) represent a family of ubiquitous plant proteins belonging to the prolamin superfamily. nsLTPs are characterized by a globular α-helical structure stabilized by four disulfide bonds and a hydrophobic cavity which acts as ligand-binding site for a broad spectrum of lipids and hydrophobic molecules. nsLTPs are involved in membrane biogenesis and in the adaption of plants to abiotic and biotic stress. They display antimicrobial activity by the ability to permeabilize the cell membrane of phytopathogens. Moreover, in the presence of lipids, nsLTPs are suggested to activate the plant immune system by a receptor-dependent mechanism. Additionally, nsLTPs from pollen and plant-derived food, in particular type 1 nsLTPs (9 kDa), are described as potent allergens. Within the nsLTP family Pru p 3 from peach is the clinically most relevant allergen which can cause genuine food allergy and frequently elicits severe clinical reactions. So far, the allergenic properties of nsLTPs are attributed to both their low molecular mass and their high thermal and proteolytic stability which allow them to reach the immune system in a biological intact form. Recently, the interaction of nsLTPs with lipids has been suggested to increase their allergenic properties and to promote the allergic sensitization to these proteins. This review will summarize the current knowledge on diversity of lipid ligands of plant LTPs, and illustrate recent studies performed with allergenic nsLTPs to investigate the effect of lipid binding on the structural modification and IgE-binding properties of proteins, and finally the potential effect on the innate immune responses.

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“…Notably a tomato LTP was found to bind L-α-palmitoyl-lysophosphatidylcholine (C16). This complex was stable even after thermal treatments with up to 105°C ( 22 ).…”

Section: Structural Interaction Of Food-derived Lipids With Nsltpsmentioning

confidence: 99%

Interaction of Non-Specific Lipid-Transfer Proteins With Plant-Derived Lipids and Its Impact on Allergic Sensitization

Scheurer

Schülke

2018

Front. Immunol.

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“…Three of the seven tomato ( Solanum lycopersicum L.) allergens registered in the “allergen.org” database are nsLTPs: Sola l 3 ( Solyc10g075090 ), Sola l 6 ( Solyc02g086310 ) and Sola l 7 ( Solyc01g090360 ). The allergen Sola l 3 was identified in the flesh and epicarp of tomato fruits, while Sola l 6 and Sola l 7 were detected in the seeds 9,12 . Despite their importance, studies on the thermal stability and structural features of these allergenic nsLTPs are still very limited 9,12 .…”

Section: Introductionmentioning

confidence: 99%

“…The strong interest of the research community towards this protein family is mainly due to the fact that nsLTPs were identified as major human allergens. In particular, these proteins are the most frequent cause of primary food allergy in adults of the Mediterranean area where they induce the largest number of food-dependent anaphylactic reactions 9,10 . Due to their high structural stability, nsLTPs resist to both heat and pepsin digestion and can act as allergens even in cooked and processed foods 9,11 .…”

Section: Introductionmentioning

confidence: 99%

“…In particular, these proteins are the most frequent cause of primary food allergy in adults of the Mediterranean area where they induce the largest number of food-dependent anaphylactic reactions 9,10 . Due to their high structural stability, nsLTPs resist to both heat and pepsin digestion and can act as allergens even in cooked and processed foods 9,11 . Three of the seven tomato ( Solanum lycopersicum L.) allergens registered in the “allergen.org” database are nsLTPs: Sola l 3 ( Solyc10g075090 ), Sola l 6 ( Solyc02g086310 ) and Sola l 7 ( Solyc01g090360 ).…”

Section: Introductionmentioning

confidence: 99%

“…The in vitro production and characterization of tomato allergens may contribute to better understand the allergenic properties of this family. Moreover, having the purified Sola l 3 protein available is a first step towards the production of monoclonal/ployclonal antibodies in order to develop novel immunoassays for tomato allergens 9,20 .…”

Section: Introductionmentioning

confidence: 99%

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Identification of non-specific Lipid Transfer Protein gene family members in Solanum lycopersicum and insights into the features of Sola l 3 protein

D’Agostino¹,

Buonanno

Ayoub

et al. 2019

Sci Rep

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Non-specific lipid transfer proteins (nsLTPs) are characterized by an eight-cysteine motif backbone that is stabilized by four disulphide bonds. The strong interest towards this protein family is mainly due to the fact that nsLTPs are involved in many biological processes and have been identified as major human allergens. Since tomato (Solanum lycopersicum L.) is one of the most consumed and allergenic vegetables, a full characterization of this family is needed. In this study, hidden Markov model profiles were used to identify nsLTPs within the tomato protein complement. Following manual curation, 64 nsLTP genes were classified into six sub-families. Furthermore, nsLTP gene structure, distribution and arrangement along tomato chromosomes were investigated. Available RNA-seq expression profile data and Real-Time PCR analyses were used to derive expression patterns of tomato nsLTPs in different tissues/organs. Non-specific LTP genes with high level of expression in tomato fruits were filtered out since they could play a key role in tomato allergenicity. Among these genes was Solyc10g075090 that encodes the allergen Sola l 3. Finally, cloning, heterologous expression, purification and biochemical characterization of the recombinant protein Sola l 3 was performed.

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The effect of organic and conventional farm management on the allergenic potency and bioactive compounds status of apricots (Prunus armeniaca L.)

et al. 2019

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Expression and characterization of a new isoform of the 9 kDa allergenic lipid transfer protein from tomato (variety San Marzano)

Cited by 8 publications

References 42 publications

Interaction of Non-Specific Lipid-Transfer Proteins With Plant-Derived Lipids and Its Impact on Allergic Sensitization

Interaction of Non-Specific Lipid-Transfer Proteins With Plant-Derived Lipids and Its Impact on Allergic Sensitization

Identification of non-specific Lipid Transfer Protein gene family members in Solanum lycopersicum and insights into the features of Sola l 3 protein

The effect of organic and conventional farm management on the allergenic potency and bioactive compounds status of apricots (Prunus armeniaca L.)

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