Expression and Clinical Significance of ACTA2 in Osteosarcoma Tissue

Tang, Lina; Hu, Haiyan; Zhou, Yan; Huang, Yujing; Wang, Yonggang; Zhang, Yawen; Liang, Jinrong

doi:10.32604/oncologie.2022.026296

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…Osteoporosis screening should be considered in hypertensive patients to enable early detection and treatment. At the same time, medical staff should pay extra attention to the screening of osteoporosis, carry out knowledge education, and tell about harms and prevention measures to patients with cardiovascular and cerebrovascular diseases, which is of great significance for early prevention and control of osteoporosis ( Tang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Association between osteoporosis and cardiovascular disease in elderly people: evidence from a retrospective study

Hu,

Ma,

Chen

et al. 2023

PeerJ

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Objective This study aimed to investigate the associations between osteoporosis, biochemical indexes, bone mineral density (BMD), and cardiovascular disease. Methods A cross-sectional study design was used to examine the relationships between these parameters. Logistic regression and correlation analyses were conducted to assess the associations between elevated levels of triglyceride, total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL), homocysteine, and the presence of osteoporosis. Additionally, correlations between BMD and biochemical indexes were analyzed. The incidence of cardiovascular disease and its correlation with BMD were evaluated. Receiver operating characteristic (ROC) analysis was performed to determine the utility of BMD in identifying cardiovascular disease. Results The results revealed that elevated triglyceride, total cholesterol, and LDL levels were positively associated with osteoporosis, while higher HDL levels and homocysteine were negatively associated. Correlation analysis demonstrated negative correlations between triglyceride levels and BMD, and positive correlations between total cholesterol and HDL levels with BMD. LDL levels showed a weak negative correlation, and homocysteine levels exhibited a strong negative correlation with BMD. The osteoporosis group had lower BMD and a higher incidence of cardiovascular disease compared to the non-osteoporosis group. Logistic regression analysis confirmed the correlation between lower BMD and increased risk of cardiovascular disease. Conclusion This study provides evidence supporting the associations between osteoporosis, biochemical indexes, BMD, and cardiovascular disease. Aberrations in lipid profiles and homocysteine levels may contribute to osteoporosis development. Lower BMD, particularly in individuals with osteoporosis, appears to increase the risk of cardiovascular disease. BMD shows promise as a diagnostic tool for identifying individuals at risk of cardiovascular disease. Further research is needed to elucidate the underlying mechanisms and establish the clinical implications of these relationships. Future longitudinal studies are necessary to determine causality and long-term prognostic implications.

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Section: Discussionmentioning

confidence: 99%

Association between osteoporosis and cardiovascular disease in elderly people: evidence from a retrospective study

Hu,

Ma,

Chen

et al. 2023

PeerJ

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“…After normalizing gene expression profiles, 2,000 highly variable genes (HVGs) were selected. For identifying marker genes in each sample, we used the "FindAllMarkers" function with adjusted p − value<0:05 and |log2 fold change| > 0.25 as cutoffs [13].…”

Section: Single-cellmentioning

confidence: 99%

Unveiling Cellular Traits of Osteosarcoma Cancer Stem Cells via Bulk and Single-Cell RNA-Sequencing Analysis

Zhao,

Niu,

et al. 2023

Stem Cells International

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Osteosarcoma (OS) is a prototypical sarcoma, predominantly affecting adolescents. The hallmark of cancer stem cell (CSC) behavior pervades OS, invariably signifying an unfavorable prognosis. However, the intricacies underlying OS metastasis remain incompletely comprehended. As the frontiers of the scientific investigation push forward, encompassing both bulk sequencing and single-cell sequencing (scRNA), the domain of bioinformatics finds multifaceted utility. In this study, we combined scRNA and bulk sequencing with the clinical metadata to excavate the latent molecular substrates governing metastatic propensities within OS. Our scRNA analysis indicated that cell-stemness-related pathways might play vital roles in OS metastasis. Subsequently, an autonomous reservoir of bulk sequencing data set was subjected to weighted gene co-expression network analysis (WGCNA) to identify 10 gene clusters. After analyzing the clinical data, we were able to identify two hub genes, HDAC2 and HSPA4, which are strongly linked to the cancer cell stemness. Moreover, we performed transwell assays to validate the regulation of metastatic behaviors by miR-1-3p, HDAC2, and HSPA4 in OS cells. Significantly, our study was fortified by immunohistochemistry (IHC) analyses performed on tumor tissues acquired from OS patients, thereby accentuating the clinical import of our experimental endeavor. Notably, we unveiled the suppressive influence of miR-1-3p on both HDAC2 and HSPA4, with miRTarBase substantiating lower expression in tumor tissue relative to the normative cohort. Insights gleaned from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) program (https://ocg.cancer.gov/programs/target) further augmented the clinical significance of the identified hub genes. In conclusion, our findings highlight the significant role of cell junctions in governing OS stemness and metastasis, underscored by the integration of single-cell sequencing and bulk-sequencing analyses. Moreover, our foundational experiments identified three hub molecules closely associated with the metastatic behaviors. Our findings provide novel insights into the clinical treatment and fundamental understanding of OS.

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“…Another incidence peak is in the older patients aging more than 65 [ 3 ]. Paget disease and radiation are two major causes of osteosarcoma [ 4 , 5 ]. Osteosarcoma as the most frequent primary tumor type in bone tumor, which largely affects in the sites of distal femur and proximal tibia [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Immunogenic cell death related mRNAs associated signature to predict immunotherapeutic response in osteosarcoma

Han,

Wang,

Shen

et al. 2024

Heliyon

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Expression and Clinical Significance of ACTA2 in Osteosarcoma Tissue

Cited by 8 publications

References 22 publications

Association between osteoporosis and cardiovascular disease in elderly people: evidence from a retrospective study

Association between osteoporosis and cardiovascular disease in elderly people: evidence from a retrospective study

Unveiling Cellular Traits of Osteosarcoma Cancer Stem Cells via Bulk and Single-Cell RNA-Sequencing Analysis

Immunogenic cell death related mRNAs associated signature to predict immunotherapeutic response in osteosarcoma

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