Expression and clinical significance of circular RNA hsa_circ_0079787 in the peripheral blood of patients with axial spondyloarthritis

Luo, Qing; Fu, Biqi; Zhang, Lu; Guo, Yang; Li, Junming

doi:10.3892/mmr.2020.11520

Cited by 7 publications

(5 citation statements)

References 35 publications

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“…Luo et al demonstrated that low expression of hsa_circ_0079787 in peripheral blood of AS patients was negatively correlated with BASDAI, which was in concert with our data. 28 Collectively, the expression level of circRNA_0003307 was associated with disease activity. 29 CircRNA_0003307 activated the PI3K/AKT signaling pathway and affected the expression of the downstream inflammatory factors -TNF-α and TNFAIP2.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of circular RNA hsa_circ_0003307 inhibits synovial inflammation in ankylosing spondylitis by regulating the PI3K/AKT pathway

Fang¹,

Liu²,

Long³

et al. 2022

Adv Clin Exp Med

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Background. Ankylosing spondylitis (AS) has a high disability rate, and an early diagnosis is difficult. Objectives.To explore the possible functions and underlying mechanism of circular RNAs Homo sapiens (hsa)_circ_0003307 in ankylosing spondylitis. Materials and methods.The hsa_circ_0003307 expression levels were investigated in the peripheral blood mononuclear cells (PBMCs) of 30 AS patients and 30 healthy controls (HC) using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. Primary fibroblast-like synoviocytes (FLS) were separated from synovial tissues, established as cell lines and cultured for subsequent cell experiments involving transfection with different vectors. The qRT-PCR analysis was used for evaluating the levels of hsa_circ_0003307 in AS-FLS. Phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway-related protein levels were measured using western blotting and immunofluorescence. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of inflammatory cytokines. Spearman's correlation analysis was used to assess the correlation between hsa_circ_0003307 and clinical characteristics.Results. The expression level of hsa_circ_0003307 was significantly high in AS patients and was positively associated with erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), and Bath Ankylosing Spondylitis Functional Index (BASFI). We found that hsa_circ_0003307 overexpression could promote the activation of the PI3K/AKT pathway and expression of inflammatory cytokines -tumor necrosis factor alpha (TNF-α) and TNF-α-induced protein 2 (TNFAIP2). However, hsa_circ_0003307 knockdown reduced the expression of TNF-α and TNFAIP2.Conclusions. The expression level of hsa_circ_0003307 was associated with inflammatory response, and it was revealed that hsa_circ_0003307 knockdown could reduce the inflammatory response of AS by regulating the PI3K/AKT pathway.

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Section: Discussionmentioning

confidence: 99%

Knockdown of circular RNA hsa_circ_0003307 inhibits synovial inflammation in ankylosing spondylitis by regulating the PI3K/AKT pathway

Fang¹,

Liu²,

Long³

et al. 2022

Adv Clin Exp Med

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Section: Introductionmentioning

confidence: 99%

“…CircRNAs have been studied as potential diagnostic markers and therapeutic molecules in these diseases. In particular, ceRNA regulation has been found to play an important role in ankylosing spondylitis (AS), which is a subtype of JSpA [4]. Several studies have investigated the regulatory role of circRNAs in AS, highlighting the potential of these molecules as therapeutic targets for the disease [5][6][7][8][9].Integrative ceRNA regulatory networks, which incorporate data on circRNA, miRNA, and mRNA, can provide valuable insights into complex gene interactions and help identify potential biomarkers for the diagnosis and treatment of diseases such as JSpA.…”

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confidence: 99%

Identifying circRNA-associated-ceRNA networks in juvenile spondyloarthropathies patients.

Wei

2023

Preprint

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Background Juvenile spondyloarthropathies (JSpA) are defined as a heterogeneous group of diseases that start before the age of 16. The study aimed to identify key genes and pathways that are influenced by circRNAs and to screen potential therapeutic agents for JSpA. The study likely involved the analysis of circRNA expression profiles, identification of circRNA-miRNA-mRNA regulatory networks, and functional annotation of differentially expressed genes. The results of the study may have provided insights into the molecular mechanisms underlying JSpA and potential therapeutic targets for this disease.Methods In this study, sequencing data of circRNA, miRNA, and mRNA were obtained from the GEO datasets. The data were then analyzed to identify candidates for constructing a circRNA-miRNA-mRNA network based on circRNA-miRNA interactions and miRNA-mRNA interactions. Functional enrichments of genes were performed using the DAVID database. A PPI network was constructed using the STRING database and visualized using Cytoscape software. The MCODE plugin app was used to explore hub genes in the PPI network. The expression changes in immune cells were assessed using the online CIBERSORT algorithm to obtain the proportion of various types of immune cells. Finally, the Connectivity Map L1000 platform was used to identify potential agents for JSpA treatment. Overall, this study aimed to provide a comprehensive understanding of the molecular mechanisms underlying JSpA and to identify potential therapeutic agents for this disease.Results A total of 225 differentially expressed circRNAs (DEcircRNAs), 23 differentially expressed miRNAs (DEmiRNAs) and 1324 differentially expressed mRNAs (DEmRNAs) were identified. We integrated 5 overlapped circRNAs, 7 miRNAs and 299 target mRNAs into a circRNA–miRNA–mRNA network. We next identified 10 hub genes based on the PPI network. KEGG pathway analysis revealed that the DEGs were mainly associated with JAK-STAT signal pathway. We found that neutrophils accounted for the majority of all infiltrating cells. In addition, we discovered several chemicals as potential treatment options for JSpA.Conclusions Through this bioinformatics analysis, we suggest a regulatory role for circRNAs in the pathogenesis and treatment of JSpA from the view of a competitive endogenous RNA (ceRNA) network.

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“…CircSIAE (hsa_circ_0000367) is a circRNA formed by reverse splitting of linear SIAE gene at the position chr11:124517260-124518071 on chromosome, consisting of 244 nucleotides. It is derived from a spline of SIAE mRNA associated with immune diseases and reported as a novel potential biomarker for emerging rheumatoid arthritis ( Luo et al., 2020 ; Zhou et al., 2020 ). In this study, we used cultured cell systems to study the effect of CVB3 infection on expression levels of circRNAs and to investigate potential downstream mechanisms of their involvement in viral processes.…”

Section: Introductionmentioning

confidence: 99%

The circRNA circSIAE Inhibits Replication of Coxsackie Virus B3 by Targeting miR-331-3p and Thousand and One Amino-Acid Kinase 2

Yang

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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Coxsackie virus B3 (CVB3), an enterovirus, is the main pathogen causing viral myocarditis, pericarditis, hepatitis and other inflammation-related diseases. Non-coding RNAs with a closed loop molecular structure, called circular RNAs (circRNAs), have been shown to be involved in multiple virus-related processes, but roles and mechanisms in CVB3 infection have not been systematically studied. In this study, when HeLa cells were infected with CVB3, the expression of hsa_circ_0000367 (circSIAE) was significantly decreased as demonstrated by real-time quantitative PCR assays. We found that circSIAE downregulated the expression of miR-331-3p through direct binding and inhibited the replication of CVB3 in HeLa and 293T cells. The analysis of signals downstream of miR-331-3p suggested that miR-331-3p promotes CVB3 replication, viral plaque formation and fluorescent virus cell production through interactions with the gene coding for thousand and one amino-acid kinase 2 (TAOK2). In conclusion, this study found that circSIAE can target TAOK2 through sponge adsorption of miR-331-3p to inhibit the replication and proliferation of CVB3 virus, providing an early molecular target for the diagnosis of CVB3 infection.

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Expression and clinical significance of circular RNA hsa_circ_0079787 in the peripheral blood of patients with axial spondyloarthritis

Cited by 7 publications

References 35 publications

Knockdown of circular RNA hsa_circ_0003307 inhibits synovial inflammation in ankylosing spondylitis by regulating the PI3K/AKT pathway

Knockdown of circular RNA hsa_circ_0003307 inhibits synovial inflammation in ankylosing spondylitis by regulating the PI3K/AKT pathway

Identifying circRNA-associated-ceRNA networks in juvenile spondyloarthropathies patients.

The circRNA circSIAE Inhibits Replication of Coxsackie Virus B3 by Targeting miR-331-3p and Thousand and One Amino-Acid Kinase 2

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